β2-adrenergic receptor redistribution in heart failure changes cAMP compartmentation

Science

Volumn 327, Issue 5973, 2010, Pages 1653-1657

  Nikolaev, Viacheslav O ^a,b   Moshkov, Alexey ^a   Lyon, Alexander R ^a   Miragoli, Michele ^a   Novak, Pavel ^a,c   Paur, Helen ^a   Lohse, Martin J ^b   Korchev, Yuri E ^c   Harding, Sian E ^a   Gorelik, Julia ^a  

^a NATIONAL HEART AND LUNG INSTITUTE   (United Kingdom)

^b UNIVERSITY OF WÜRZBURG   (Germany)

^c IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BETA 2 ADRENERGIC RECEPTOR; CYCLIC AMP; BETA 1 ADRENERGIC RECEPTOR; CYCLIC AMP DEPENDENT PROTEIN KINASE;

CARDIOVASCULAR DISEASE; HEALTH RISK; ION; MICROSCOPY; PROTEIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL SURFACE; CONTROLLED STUDY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HEART FAILURE; HEART MUSCLE CELL; ION CONDUCTANCE; MOUSE; NANOANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; RAT; SIGNAL TRANSDUCTION; ANIMAL; CELL COMPARTMENTALIZATION; CELL MEMBRANE; CHRONIC DISEASE; CYTOSOL; GENETICS; MALE; METABOLISM; METHODOLOGY; MICROSCOPY; MOUSE MUTANT; PATHOLOGY; SARCOLEMMA; SPRAGUE DAWLEY RAT; TRANSGENIC MOUSE; ULTRASTRUCTURE;

MUS; RATTUS;

ANIMALS; CELL COMPARTMENTATION; CELL MEMBRANE; CHRONIC DISEASE; CYCLIC AMP; CYCLIC AMP-DEPENDENT PROTEIN KINASES; CYTOSOL; FLUORESCENCE RESONANCE ENERGY TRANSFER; HEART FAILURE; MALE; MICE; MICE, KNOCKOUT; MICE, TRANSGENIC; MICROSCOPY; MYOCYTES, CARDIAC; RATS; RATS, SPRAGUE-DAWLEY; RECEPTORS, ADRENERGIC, BETA-1; RECEPTORS, ADRENERGIC, BETA-2; SARCOLEMMA; SIGNAL TRANSDUCTION;

EID: 77950286813     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1185988     Document Type: Article

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31. We thank P. O'Gara for cardiomyocyte isolation; C. Dees for receptor binding assays; and L. Jaffe, F. Klauschen, and S. Gambaryan for critical reading of the manuscript. This study was supported by the Wellcome Trust (WTN 084064 to J.G.), Action Medical Research Grant (to J.G.), a U.K. Biotechnology and Biological Sciences Research Council grant (to Y.E.K.), a U.K. Medical Research Council grant (to J.G. and Y.E.K.) and a grant from Leducq Foundation (to S.E.H. and M.J.L.). Y.E.K. is a shareholder of, and P.N. has a consultancy agreement with, lonscope Ltd., a company that sells scanning ion conductance microscopes. The cAMP sensor Epac2-camps used in this study is covered by a patent belonging to the University of Würzburg. It is being provided at no cost, but with a material transfer agreement, to nonprofit research institutes and universities.