A dual estrogen receptor TR-FRET assay for simultaneous measurement of steroid site binding and coactivator recruitment

Journal of Biomolecular Screening

Volumn 15, Issue 3, 2010, Pages 268-278

  Hilal, Tarek ^a   Puetter, Vera ^b   Otto, Christiane ^a   Parczyk, Karsten ^a   Bader, Benjamin ^a  

^a BAYER PHARMA AG   (Germany)

^b Lead Generation and Optimization   (Germany)

Author keywords

Coactivator recruitment; Estrogen receptor; Multiplex; Terbium; TR FRET

Indexed keywords

4 HYDROXYTAMOXIFEN; BAZEDOXIFENE; DIETHYLSTILBESTROL; ESTRADIOL; ESTROGEN; ESTROGEN RECEPTOR; ESTROGEN RECEPTOR ALPHA; ESTROGEN RECEPTOR BETA; ESTRONE; ETHINYLESTRADIOL; FLUORESCEIN; FULVESTRANT; GLUTATHIONE TRANSFERASE; LIGAND; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA COACTIVATOR 1ALPHA; RALOXIFENE; RECOMBINANT PROTEIN; STEROID; SYNTHETIC PEPTIDE; TERBIUM;

ARTICLE; ASSAY; BINDING SITE; DOSE RESPONSE; DRUG TARGETING; FLUORESCENCE RESONANCE ENERGY TRANSFER; MEASUREMENT; PRIORITY JOURNAL; TECHNOLOGY;

BINDING SITES; BIOLOGICAL ASSAY; ESTROGEN RECEPTOR ALPHA; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; PEPTIDES; PROTEIN BINDING; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; STEROIDS; TERBIUM; TRANSCRIPTION FACTORS;

EID: 77949409085     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057109359196     Document Type: Article

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