Multiplexing fluorescence polarization assays to increase information content per screen: Applications for screening steroid hormone receptors

Journal of Biomolecular Screening

Volumn 9, Issue 4, 2004, Pages 294-302

  Blommel, Paul ^a,b   Hanson, George T ^a   Vogel, Kurt W ^a  

^a INVITROGEN CORPORATION   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Estrogen receptor ; Fluorescence polarization; Multiplexed assays; Nuclear receptors; Progesterone receptor

Indexed keywords

1,3,5 TRIS(4 HYDROXYPHENYL) 4 PROPYL 1H PYRAZOLE; 2 METHOXYESTRADIOL; 2,3,4,5 TETRAHYDRO 7,8 DIHYDROXY 1 PHENYL 1H 3 BENZAZEPINE; 7,7 DIMETHYLEICOSADIENOIC ACID; BUDESONIDE; CHRYSENE DERIVATIVE; CORTICOSTERONE; CYPROTERONE ACETATE; DIETHYLTETRAHYDRO 2,8 CHRYSENEDIOL; ESTRADIOL; ESTROGEN RECEPTOR ALPHA; ESTRONE; FLUORESCEIN; HORMONE RECEPTOR BLOCKING AGENT; HYDROXYPROGESTERONE; LIGAND; LUTEOLIN; MIFEPRISTONE; PRASTERONE; PROGESTERONE; PROGESTERONE RECEPTOR; PYRAZOLE DERIVATIVE; RALOXIFENE; REAGENT; ROTTLERIN; SPIRONOLACTONE; STEROID RECEPTOR; TAMOXIFEN CITRATE; UNCLASSIFIED DRUG; 5 CARBOXYTETRAMETHYLRHODAMINE SUCCINIMIDYL ESTER; 5-CARBOXYTETRAMETHYLRHODAMINE SUCCINIMIDYL ESTER; FLUORESCENT DYE; RECOMBINANT PROTEIN; RHODAMINE;

ANALYTIC METHOD; ARTICLE; COST CONTROL; DRUG RECEPTOR BINDING; DRUG SELECTIVITY; FLUORESCENCE POLARIZATION; FOLLOW UP; HIGH THROUGHPUT SCREENING; LIQUID; PRIORITY JOURNAL; DRUG EFFECT; DRUG SCREENING; HUMAN; IN VITRO STUDY; METABOLISM; METHODOLOGY;

DRUG EVALUATION, PRECLINICAL; ESTROGEN RECEPTOR ALPHA; FLUORESCEIN; FLUORESCENCE POLARIZATION; FLUORESCENT DYES; HUMANS; LIGANDS; RECEPTORS, PROGESTERONE; RECEPTORS, STEROID; RECOMBINANT PROTEINS; RHODAMINES;

EID: 16544373673     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1177/1087057104264420     Document Type: Article

References (19)

1. Schriemer DC, Hindsgaul O: Deconvolution approaches in screening compound mixtures. Comb Chem High Throughput Screen 1998;1(4):155-170.
2. Snider M: Screening of Compound Libraries ... Consommé or Gumbo? J Biomol Screen 1998;3:169-170.
3. Chung TDY: Screen compounds singly: why muck it up? J Biomol Screen 1998;3:171-173.
4. Mendoza LG, McQuary P, Mongan A, Gangadharan R, Brignac S, Eggers M: High-throughput microarray-based enzyme-linked immunosorbent assay (ELISA). Biotechniques 1999;27(4):778-788.
5. Xu YY, Pettersson K, Blomberg K, Hemmila I, Mikola H, Lovgren T: Simultaneous quadruple-label fluorometric immunoassay of thyroid-stimulating hormone. 17 alpha-hydroxyprogesterone, immunoreactive trypsin, and creatine kinase MM isoenzyme in dried blood spots. Clin Chem 1992;38(10):2038-2043.
6. Swartzman EE, Miraglia SJ, Mellentin-Michelotti J, Evangelista L, Yuan PM: A homogeneous and multiplexed immunoassay for high-throughput screening using fluorometric microvolume assay technology. Anal Biochem 1999;271(2):143-151.
7. Tannous BA, Verhaegen M, Christopoulos TK, Kourakli A: Combined flash- and glow-type chemiluminescent reactions for high-throughput genotyping of biallelic polymorphisms. Anal Biochem 2003;320(2):266-272.
8. Fulton RJ, McDade RL, Smith PL, Kienker LJ, Kettman JR Jr: Advanced multiplexed analysis with the FlowMeirix system. Clin Chem 1997;43(9):1749-1756.
9. Lynch BA, Minor C, Loiacono KA, van Schravendijk MR, Ram MK, Sundaramoorthi R, et al: Simultaneous assay of Src SH3 and SH2 domain binding using different wavelength fluorescence polarization probes. Anal Biochem 1999;275:62-73.
10. Chen X, Levine L, Kwok P-Y: Fluorescence polarization in homogenous nucleic acid analysis. Genome Res 1999;(9):492-498.
11. Grover GS, Turner BA, Parker CN, Meier J, Lala DS, Lee PH: Multiplexing nuclear receptors for agonist identification in a cell-based reporter gene high-throughput screen. J Biomol Screen 2003;8(3):239-246.
12. Parker GJ, Law TL, Lenoch FJ, Bolger RE: Development of high throughput screening assays using fluorescence polarization: nuclear receptor ligand binding and kinase/phosphatase assays. J Biomol Screen 2000;5:77-88.
13. Turconi S, Shea K, Ashman S, Fantom K, Earnshaw DL, Bingham RP et al: Real experiences of uHTS: a prototypic 1536-well fluorescence anisotropy-based uHTS screen and application of well-level quality control procedures. J Biomol Screen 2001;6:275-290.
14. Motlusky H: Analyzing Data With GraphPad Prism. San Diego, CA: GraphPad Software, 1999. Available at www.graphpad.com
15. Zhang JH, Chung TD, Oldenburg KR: A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 1999;4:67-73.
16. Sibley DR, Left SE, Creese I: Interactions of novel dopaminergic ligands with D-1 and D-2 dopamine receptors. Life Sci 1982;31:637-645.
17. Donze O, Picard D: Ligand-independent activation of the estrogen receptor: activation by epidermal growth factor and dopamine. Ernst Schering Res Found Workshop 1998;24:109-120.
18. 5 dopamine receptors mediate estrogen-induced stimulation of hypothalamic atrial natriuretic factor neurons. Mol Endocrinol 1999;13(2):344-352.
19. Tanenbaum DM, Wang Y, Williams SP, Sigler PB: Crystallographic comparison of the estrogen and progesterone receptor's ligand binding domains. Proc Natl Acad Sci U S A 1998;95(11):5998-6003.