Flow cytometry-based methods for assessing soluble scFv activities and detecting antigens in solution

Biotechnology and Bioengineering

Volumn 105, Issue 5, 2010, Pages 973-981

  Gray, Sean A ^a   Weigel, Kris M ^a   Miller, Keith D ^b   Ndung'U, Joseph ^c   Büscher, Philippe ^d   Tran, Thao ^d,e   Baird, Cheryl ^b   Cangelosi, Gerard A ^a  

^a SEATTLE BIOMEDICAL RESEARCH INSTITUTE   (United States)

^b PACIFIC NORTHWEST NATIONAL LABORATORY   (United States)

^c FOUNDATION FOR INNOVATIVE NEW DIAGNOSTICS   (Switzerland)

^d INSTITUTE OF TROPICAL MEDICINE   (Belgium)

^e VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

Author keywords

African trypanosomiasis; Antibodies; Competitive inhibition; Flow cytometry; Invariant surface glycoproteins; ISG; S. cerevisiae; scFv; Trypanosoma; Yeast display

Indexed keywords

COMPETITIVE INHIBITION; INVARIANT SURFACES; ISG; S.CEREVISIAE; TRYPANOSOMA;

ANTIBODIES; ANTIGENS; ASSAYS; FLOW CYTOMETRY; GLYCOPROTEINS; LIBRARIES; SULFUR COMPOUNDS;

YEAST;

EPITOPE; GLYCOPROTEIN;

ANTIGEN BINDING; ANTIGEN DETECTION; ANTIGEN EXPRESSION; ANTIGEN PURIFICATION; ARTICLE; BIOTINYLATION; CONTROLLED STUDY; FLOW CYTOMETRY; IMMUNOASSAY; NONHUMAN; TRYPANOSOMA BRUCEI;

ANIMALS; ANTIGENS, PROTOZOAN; FLOW CYTOMETRY; IMMUNOASSAY; IMMUNOGLOBULIN VARIABLE REGION; PROTEIN BINDING; SOLUTIONS; TRYPANOSOMA BRUCEI BRUCEI; YEASTS;

TRYPANOSOMA; TRYPANOSOMA BRUCEI;

EID: 77949359825     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22607     Document Type: Article

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