Heterologous expression, purification and characterisation of the extracellular domain of trypanosome invariant surface glycoprotein ISG75

Journal of Biotechnology

Volumn 135, Issue 3, 2008, Pages 247-254

  Tran, Thao ^a,b   Büscher, Philippe ^c   Vandenbussche, Guy ^d   Wyns, Lode ^a,b   Messens, Joris ^a,b   De Greve, Henri ^a,b  

^a DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

^b VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

^c INSTITUTE OF TROPICAL MEDICINE   (Belgium)

^d UNIVERSITÉ LIBRE DE BRUXELLES   (Belgium)

Author keywords

Heterologous expression; Recombinant ISG75; Sera; Stabilisation agents; Trypanosoma brucei

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; CHEMICALS; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; CIRCULAR DICHROISM SPECTROSCOPY; DICHROISM; ENZYME ACTIVITY; ESCHERICHIA COLI; GLYCOPROTEINS; HEALTH; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; LIQUID PHASE EPITAXY; MASS SPECTROMETRY; OPTICAL PROPERTIES; PRESSURE; PURIFICATION; SPECTRUM ANALYSIS; SURFACE PROPERTIES; SURFACES;

(1 1 0) SURFACE; BUFFER SOLUTIONS; CDNA FRAGMENT; CHARACTERISATION; CIRCULAR DICHROISM (DC); DIAGNOSTIC TESTING; ELSEVIER (CO); EXTRACELLULAR DOMAINS; FOLDED CONFORMATION; GLUTATHIONE (GSH); HETEROLOGOUS EXPRESSIONS; HIGH-PRESSURE LIQUID; HISTIDINE (HIS); L ARGININE; L GLUTAMIC ACID (PGA); N TERMINALS; OXIDOREDUCTASE; RABBIT ANTISERUM; SOLUBLE PROTEINS; THIOREDOXIN REDUCTASE (TRXR); TRANSMEMBRANE (TM); TRYPANOSOMA BRUCEI;

HIGH PRESSURE LIQUID CHROMATOGRAPHY;

ARGININE; COMPLEMENTARY DNA; DNA FRAGMENT; GLUTAMIC ACID; GLUTATHIONE REDUCTASE; GLYCOPROTEIN; HISTIDINE; ISG75 GLYCOPROTEIN; MESSENGER RNA; STREPTAVIDIN; THIOREDOXIN REDUCTASE;

AFFINITY CHROMATOGRAPHY; ARTICLE; BACTERIAL STRAIN; CIRCULAR DICHROISM; CYTOPLASM; ESCHERICHIA COLI; MASS SPECTROMETRY; NONHUMAN; PARASITOSIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; REVERSED PHASE LIQUID CHROMATOGRAPHY; RNA TRANSLATION; TRYPANOSOMA; TRYPANOSOMOSIS;

AMINO ACID SEQUENCE; ANIMALS; CHROMATOGRAPHY, AFFINITY; CIRCULAR DICHROISM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GOATS; MASS SPECTROMETRY; MEMBRANE GLYCOPROTEINS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PEPTIDES; PROTEIN STRUCTURE, TERTIARY; PROTOZOAN PROTEINS; TRYPANOSOMA BRUCEI BRUCEI;

ANIMALIA; ESCHERICHIA COLI; ORYCTOLAGUS CUNICULUS; TRYPANOSOMA BRUCEI; TRYPANOSOMA BRUCEI GAMBIENSE; TRYPANOZOON;

EID: 45449094364     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2008.04.012     Document Type: Article

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