Structural basis for inverting the enantioselectivity of arylmalonate decarboxylase revealed by the structural analysis of the Gly74Cys/Cysl88Ser mutant in the liganded form

Biochemistry

Volumn 49, Issue 9, 2010, Pages 1963-1969

  Obata, Rika ^a   Nakasako, Masayoshi ^a,b  

^a KEIO UNIVERSITY   (Japan)

^b RIKEN HARIMA INSTITUTE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; CARBON ATOMS; DECARBOXYLASES; ENANTIOSELECTIVE; HYDROPHOBIC CLUSTERS; INVERSION IN ENANTIOSELECTIVITY; LIGAND BINDING; LIGAND MOLECULES; MIRROR SYMMETRY; OXYANION HOLE; PHENYL ACETATE; PROTONATED; RATIONAL DESIGN; REACTION INTERMEDIATE; STRUCTURAL BASIS; TWO DOMAINS; WILD TYPES; WILD-TYPE ENZYMES;

CARBOXYLATION; CONFORMATIONS; ELECTRON TRANSITIONS; ENANTIOSELECTIVITY; ENZYMES; LIGANDS; REACTION INTERMEDIATES; STRUCTURAL ANALYSIS;

CRYSTAL STRUCTURE;

ARYLMALONATE DECARBOXYLASE; BENZYLPHOSPHONATE; CARBOXYLYASE; CYSTEINE; GLYCINE; PHENYLACETIC ACID; PHOSPHONIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; ATOM; CRYSTAL STRUCTURE; DECARBOXYLATION; ENANTIOSELECTIVITY; LIGAND BINDING; MUTATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTON TRANSPORT; STRUCTURE ANALYSIS;

AMINO ACID SUBSTITUTION; BORDETELLA BRONCHISEPTICA; CARBOXY-LYASES; CATALYTIC DOMAIN; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; CYSTEINE; GLYCINE; LIGANDS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTONS; SERINE; STEREOISOMERISM;

EID: 77749259029     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi9015605     Document Type: Article

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