Detection by whole genome microarrays of a spontaneous 126-gene deletion during construction of a ytfE mutant: confirmation that a ytfE mutation results in loss of repair of iron-sulfur centres in proteins damaged by oxidative or nitrosative stress

Journal of Microbiological Methods

Volumn 81, Issue 1, 2010, Pages 77-79

  Vine, Claire E ^a   Justino, Marta C ^b   Saraiva, Lígia M ^b   Cole, Jeffrey ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^b INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

Author keywords

Genomic hybridization; Nitrosative stress; Oxidative stress; RIC protein; Spontaneous deletion

Indexed keywords

BACTERIAL PROTEIN; HYDROGEN PEROXIDE; IRON SULFUR PROTEIN; UNCLASSIFIED DRUG; YTFE PROTEIN;

ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI K 12; GENE DELETION; GENE MUTATION; GENOMIC HYBRIDIZATION; GROWTH INHIBITION; HYBRIDIZATION; MICROARRAY ANALYSIS; NITROSATIVE STRESS; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL;

ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HYDROGEN PEROXIDE; IRON; MUTATION; NUCLEIC ACID HYBRIDIZATION; SEQUENCE DELETION; SULFUR;

ESCHERICHIA COLI;

EID: 77649341609     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2010.01.023     Document Type: Article

References (17)

1. Constantinidou C.C., Hobman J.L., Patel M.D., Penn C.W., Cole J.A., and Overton T.W. A reassessment of the fumarate and nitrate reduction regulon and transcriptomic analysis of the effects of nitrate, nitrite, NarXL and NarQP as Escherichia coli adapts from aerobic to anaerobic growth. Journal of Biological Chemistry 281 (2006) 4802-4808
2. Datsenko K.A., and Wanner B.L. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proceedings of the National Academy of Sciences of the USA 97 (2000) 6640-6645
3. Filenko N., Spiro S., Browning D., Squire D., Overton T., Cole J., and Constantinidou C. The NsrR regulon of Escherichia coli K-12 includes genes encoding the hybrid cluster protein and the periplasmic, respiratory nitrite reductase. Journal of Bacteriology 189 (2007) 4410-4417
4. Flatley J., Barrett J., Pullan S.T., Hughes M.N., Green J., and Poole R.K. Transcriptional responses of Escherichia coli to S-nitrosoglutathione under defined chemostat conditions reveal major changes in methionine biosynthesis. Journal of Biological Chemistry 280 (2005) 10065-10072
5. Hobman J.L., Patel M.D., Hidalgo-Arroyo G.A., Cariss S.J.L., Avison M.B., Penn C.W., and Constantinidou C. Comparative genomic hybridisation detects secondary chromosomal deletions in Escherichia coli K-12 MG1655 mutants and highlights instability in the flhDC region. Journal of Bacteriology 189 (2007) 8786-8792
6. Justino M.C., Vicente J.B., Texiera M., and Saraiva L.M. New genes implicated in the protection of anaerobically grown Escherichia coli against nitric oxide. Journal of Biological Chemistry 280 (2005) 2636-2643
7. Justino M.C., Almeida C.C., Goncalves V.L., Teixeira M., and Saraiva L.M. Escherichia coli YtfE is a di-iron protein with an important function in assembly of iron sulfur clusters. FEMS Microbiology Letters 257 (2006) 278-284
8. Justino M.C., Almeida C.C., Teixeira M., and Saraiva L.M. Escherichia coli di-iron YtfE protein is necessary for the repair of stress-damaged iron-sulfur clusters. Journal of Biological Chemistry 282 (2007) 10352-10359
9. Mukhopadhyay P., Zheng M., Bedzyk L.A., LaRossa R.A., and Storz G. Prominent roles of the NorR and Fur regulators in the Escherichia coli transcriptional response to reactive nitrogen species. Proceedings of the National Academy of Sciences of the USA 101 (2004) 745-750
10. Outten F.W., Djaman O., and Storz G. A suf operon requirement for Fe-S cluster assembly during iron starvation in Escherichia coli. Molecular Microbiology 52 (2004) 861-872
11. Overton T.W., Justino M.C., Li Y., Baptista J.M., Melo A., Cole J.A., and Saraiva L.M. Widespread distribution in pathogenic bacteria of di-iron proteins that repair oxidative and nitrosative damage to iron-sulfur centers. Journal of Bacteriology 190 (2008) 2004-2013
12. Pope N.R., and Cole J.A. Pyruvate and ethanol as electron donors for nitrite reduction by Escherichia coli K12. Journal of General Microbiology 130 (1984) 1279-1284
13. Poteete A.R., Fenton A.C., and Nadkarni A. Chromosomal duplications and cointegrates generated by the bacteriophage lambda red system in Escherichia coli K-12. BMC Molecular Biology 5 (2004) 22
14. Poteete A.R., Wang H.R., and Foster P.L. Phage λ red-mediated adaptive mutation. Journal of Bacteriology 184 (2002) 3753-3755
15. Pullan S.T., Gidley M.D., Jones R.A., Barrett J., Stevanin T.A., Read R.C., Green J., and Poole R.K. Nitric oxide in chemostat-cultured Escherichia coli is sensed by Fnr and other global regulators: unaltered methionine biosynthesis indicates lack of S nitrosation. Journal of Bacteriology 189 (2007) 1845-1855
16. Pullan S., Monk T.C.E., Lee L., and Poole R.K. Microbial responses to nitric oxide and nitrosative stress: growth, "omic", and physiological methods. Methods in Enzymology 437 (2008) 499-519
17. Woods S.A., Schwartzbach S.D., and Guest J.R. Two biochemically distinct classes of fumarase in Escherichia coli. Biochimica et Biophysica Acta 954 (1988) 14-26