Immobilized aculeacin A acylase from Actinoplanes utahensis: Characterization of a novel biocatalyst

Bioresource Technology

Volumn 101, Issue 12, 2010, Pages 4261-4268

  Hormigo, Daniel ^a   de la Mata, Isabel ^a   Acebal, Carmen ^a   Arroyo, Miguel ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

Author keywords

Actinoplanes utahensis; Aculeacin A acylase; Confocal scanning microscopy; Immobilization; Sepabeads

Indexed keywords

6-AMINOPENICILLANIC ACID (6-APA); ACTIVITY PROFILE; ACYL MOIETY; AMIDOHYDROLASE; ANTI-FUNGAL; BATCH REACTIONS; CATALYTIC ACTIVITY; CONFOCAL SCANNING MICROSCOPY; ECHINOCANDIN; ENZYMATIC ACTIVITIES; EPOXY-ACTIVATED SUPPORT; IMMOBILIZED BIOCATALYSTS; IMMOBILIZED ENZYME; INDUSTRIAL PREPARATION; PENICILLIN V; RECOMBINANT ENZYMES; SEPABEADS; SUBSTRATE SPECIFICITY;

ANTIBIOTICS; ENZYMES; HYDROLYSIS; SCANNING;

SEWAGE TREATMENT PLANTS;

ACULEACIN A ACYLASE; AMIDASE; PENICILLIN K; PENICILLIN V; UNCLASSIFIED DRUG;

ANTIBIOTICS; BIOREACTOR; CATALYST; ENZYME; HYDROLYSIS; IMMOBILIZATION;

ACTINOPLANES UTAHENSIS; ARTICLE; BATCH REACTOR; BIOCATALYST; CONFOCAL LASER MICROSCOPY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME IMMOBILIZATION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SUBSTRATE; GRAM POSITIVE BACTERIUM; HYDROLYSIS; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; TEMPERATURE;

AMIDOHYDROLASES; BIOCATALYSIS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; ENZYME STABILITY; ENZYMES, IMMOBILIZED; HYDROGEN-ION CONCENTRATION; MICROMONOSPORACEAE; MICROSCOPY, CONFOCAL; MICROSPHERES; RECOMBINANT PROTEINS; SOLUBILITY; SUBSTRATE SPECIFICITY; TEMPERATURE;

ACTINOPLANES UTAHENSIS;

EID: 77649337919     PISSN: 09608524     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biortech.2010.01.117     Document Type: Article

References (30)

1. Arroyo M., de la Mata I., Acebal C., and Castillón M.P. Biotechnological applications of penicillin acylases: state-of-the-art. Appl. Microbiol. Biotechnol. 60 (2003) 507-514
2. Bastida A., Sabuquillo P., Armisen P., Fernández-Lafuente R., Huguet J., and Guisán J.M. A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports. Biotechnol. Bioeng. 58 (1998) 486-493
3. Boek L.D., Fukuda D.S., Abbot B.J., and Debono M. Deacylation of echinocandin B by Actinoplanes utahensis. J. Antibiot. 42 (1989) 382-388
4. Boller T., Meier C., and Menzler S. Eupergit oxirane acrylic beads: how to make enzymes fit for biocatalysis. Org. Process Res. Dev. 6 (2002) 509-519
5. Bradford M.M. A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-252
6. Chauhan S., Nichkawade A., Lyengar M.R., and Chattoo B.B. Chainia penicillin V acylase: strain characteristics, enzyme immobilization, and kinetic studies. Curr. Microbiol. 37 (1998) 186-190
7. Debono M., Abbott B.J., Fukuda D.S., Barnhart M., Willard K.E., Molloy R.M., Michel K.H., Turner J.R., Butler T.F., and Hunt A.H. Synthesis of new analogues of echinocandin B by enzymatic deacylation and chemical reacylation of the echinocandin B peptide: synthesis of the antifungal agent cyclofungin (LY121019). J. Antibiot. 42 (1989) 389-397
8. Fernández-Lafuente R., Hernández-Justiz O., Mateo C., Terreni M., Fernández-Lorente G., Moreno M.A., Alonso J., García-López J.L., and Guisán J.M. Biotransformations catalyzed by multimeric enzymes: stabilization of tetrameric ampicillin acylase permits the optimization of ampicillin synthesis under dissociation conditions. Biomacromolecules 2 (2001) 95-104
9. Ghazi I., De Segura A.G., Fernández-Arrojo L., Alcalde M., Yates M., Rojas-Cervantes M.L., Plou F.J., and Ballesteros A. Immobilisation of fructosyltransferase from Aspergillus aculeatus on epoxy-activated Sepabeads EC for the synthesis of fructo-oligosaccharides. J. Mol. Catal. B: Enzym. 35 (2005) 19-27
10. Gómez de Segura A., Alcalde M., Yates M., Rojas-Cervantes M.L., López-Cortes N., Ballesteros A., and Plou F.J. Immobilization of dextransucrase from Leuconostoc mesenteroides NRRL B-512F on Eupergit C supports. Biotechnol. Prog. 20 (2004) 1414-1420
11. Henley J.P., and Sadana A. Categorization of enzyme deactivations using a series-type mechanism. Enzyme Microb. Technol. 7 (1985) 50-60
12. Hernaiz M.J., and Crout D.H.G. Immobilization/stabilization on Eupergit C of the β-galactosidase from Bacillus circulans and an α-galactosidase from Aspergillus oryzae. Enzyme Microb. Technol. 27 (2000) 26-32
13. Hormigo D., de la Mata I., Castillón M.P., Acebal C., and Arroyo M. Kinetic and microstructural characterization of immobilized penicillin acylase from Streptomyces lavendulae on Sepabeads EC-EP. Biocatal. Biotransform. 27 (2009) 271-281
14. Karboune S., Neufeld R., and Kermasha S. Immobilization and biocatalysis of chlorophyllase in selected organic solvent systems. J. Biotechnol. 120 (2005) 273-283
15. Katchalski-Katzir E. Immobilized enzymes: learning from past successes and failures. Trends Biotechnol. 11 (1993) 471-478
16. Katchalski-Katzir E., and Kraemer D.M. Eupergit C, a carrier for immobilization of enzymes of industrial potential. J. Mol. Catal. B: Enzym. 10 (2000) 157-176
17. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
18. Mateo C., Abian O., Fernández-Lafuente R., and Guisán J.M. Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment. Enzyme Microb. Technol. 26 (2000) 509-515
19. Mateo C., Abian O., Fernández-Lorente G., Pedroche J., Fernández-Lafuente R., and Guisán J.M. Epoxy Sepabeads: a novel epoxy support for stabilization of industrial enzymes via very intense multipoint covalent attachment. Biotechnol. Prog. 18 (2002) 629-634
20. Mizuno K., Yagi A., Satoi S., Takada M., and Hayashi M. Studies on aculeacin. I. Isolation and characterization of aculeacin A. J. Antibiot. 30 (1977) 297-302
21. Sánchez-Puelles J.M., Sanz J.M., García J.L., and García E. Immobilization and single-step purification of fusion proteins using DEAE-cellulose. Eur. J. Biochem. 198 (1992) 153-159
22. Shewale J.G., Kumar K.K., and Ambedkar G.R. Evaluation of determination of 6-amino penicillanic acid by p-dimethylamino benzaldehyde. Biotechnol. Tech. 1 (1987) 69-72
23. Singh D., Goel R., and Johri B.N. Deacylation of penicillins by the immobilized mycelia of the thermophile Malbranchea. J. Gen. Appl. Microbiol. 34 (1988) 333-339
24. Sudhakaran V.K., and Shewale J.G. Enzymatic splitting of penicillin V for the production of 6-APA using immobilized penicillin V acylase. World J. Microbiol. Biotechnol. 9 (1993) 630-634
25. Takeshima H., Inokoshi J., Takada Y., Tanaka H., and Omura S. A deacylation enzyme for aculeacin A, a neutral lipopeptide antibiotic, from Actinoplanes utahensis: purification and characterization. J. Biochem. 105 (1989) 606-610
26. Torres R., Ramón F., de la Mata I., Acebal C., and Castillón M.P. Enhanced production of penicillin V acylase from Streptomyces lavendulae. Appl. Microbiol. Biotechnol. 53 (1999) 81-84
27. Torres-Bacete J., Arroyo M., Torres-Guzmán R., de la Mata I., Castillón M.P., and Acebal C. Covalent immobilization of penicillin acylase from Streptomyces lavendulae. Biotechnol. Lett. 22 (2000) 1011-1014
28. Torres-Bacete J., Arroyo M., Torres-Guzmán R., de la Mata I., Castillón M.P., and Acebal C. Optimization of 6-aminopenicillanic acid (6-APA) production by using a new immobilized penicillin acylase. Biotechnol. Appl. Biochem. 32 (2000) 173-177
29. Torres-Bacete J., Hormigo D., Stuart M., Arroyo M., Torres P., Castillón M.P., Acebal C., García J.L., and de la Mata I. Newly discovered penicillin acylase activity of aculeacin A acylase from Actinoplanes utahensis. Appl. Environ. Microbiol. 73 (2007) 5378-5381
30. Torres-Guzmán R., de la Mata I., Torres-Bacete J., Arroyo M., Castillón M.P., and Acebal C. Substrate specificity of penicillin acylase from Streptomyces lavendulae. Biochem. Biophys. Res. Commun. 291 (2002) 593-597