Contribution of cell culture, RNA extraction, and reverse transcription to the measurement error in quantitative reverse transcription polymerase chain reaction-based gene expression quantification

Analytical Biochemistry

Volumn 393, Issue 1, 2009, Pages 29-35

  Combes, Jean Damien ^a,b,c,d,e   Grelier, Gaël ^b,c,f   Laversanne, Matthieu ^a,b,c,d   Voirin, Nicolas ^b,c,d,e,g   Chabaud, Sylvie ^e   Ecochard, René ^a,b,c,d   Lasset, Christine ^b,c,d,e   Moyret Lalle, Caroline ^b,c,f  

^a HOSPICES CIVILS DE LYON   (France)

^b UNIVERSITÉ DE LYON   (France)

^c UNIVERSITÉ LYON 1   (France)

^d LABORATOIRE DE BIOMÉTRIE ET BIOLOGIE EVOLUTIVE   (France)

^e CENTRE LÉON BÉRARD   (France)

^f INSERM   (France)

^g EDOUARD HERRIOT HOSPITAL   (France)

Author keywords

Accuracy; Breast cancer; Gene expression; Measurement error; Reproducibility; RT qPCR; Variability

Indexed keywords

CELL CULTURE; DISEASES; EXTRACTION; GENE EXPRESSION; MEASUREMENT ERRORS; RNA; TRANSCRIPTION;

ACCURACY; BREAST CANCER; REPRODUCIBILITIES; RT-QPCR; VARIABILITY;

POLYMERASE CHAIN REACTION;

ANAPHASE PROMOTING COMPLEX; PROTEIN APC 11; RNA; UNCLASSIFIED DRUG;

ARTICLE; BREAST CANCER; CANCER CELL CULTURE; CATALYSIS; CELL DIVISION; CONTROLLED STUDY; ENZYME SUBUNIT; GENE EXPRESSION PROFILING; HUMAN; HUMAN CELL; MEASUREMENT ERROR; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION;

EID: 67651113763     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.06.010     Document Type: Article

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