Microplate spectrophotometry for high-throughput screening of cytotoxic molecules

Cell Proliferation

Volumn 43, Issue 2, 2010, Pages 130-138

  Tomelleri, C ^a   Dalla Pellegrina, C ^a   Chignola, R ^a,b  

^a UNIVERSITY OF VERONA   (Italy)

^b SEZIONE DI TRIESTE   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

COLCHICINE; IDARUBICIN; PACLITAXEL; TRYPAN BLUE;

ARTICLE; CELL COUNT; CELL POPULATION; CELL PROLIFERATION; CONTROLLED STUDY; DRUG CYTOTOXICITY; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; KINETICS; OPTICAL DENSITY; SPECTROPHOTOMETRY;

ANIMALS; B-LYMPHOCYTES; CELL ADHESION; CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; CELL PROLIFERATION; COLCHICINE; CULTURE MEDIA; CYTOTOXINS; EPITHELIAL CELLS; HELA CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; IDARUBICIN; KINETICS; LEUKEMIA, BIPHENOTYPIC, ACUTE; PACLITAXEL; SENSITIVITY AND SPECIFICITY; SPECTROPHOTOMETRY; T-LYMPHOCYTES; TIME FACTORS;

ANIMALIA;

EID: 77649203114     PISSN: 09607722     EISSN: 13652184     Source Type: Journal    
DOI: 10.1111/j.1365-2184.2009.00665.x     Document Type: Article

References (21)

1. Pereira DM, Williams JA (2007) Origin and evolution of high throughput screening. Br. J. Pharmacol. 152, 53 61.
2. Inglese J, Auld DS, Jadhav A, Johnson RL, Simeonov A, Yasgar A et al. (2006) Quantitative high-throughput screening: a titration-based approach that efficiently identifies biological activities in large chemical libraries. Proc. Natl. Acad. Sci. USA 103, 11473 11478.
3. Freshney IR (2000) Culture of Animal Cells: A Manual of Basic Techniques, 4th edn. New York, USA : Wiley-Liss. pp. 577
4. Slater K (2001) Cytotoxicity tests for high-throughput drug discovery. Curr. Opin. Biotechnol. 12, 70 74.
5. King M (2000) Detection of dead cells and measurement of cell killing by flow cytometry. J. Immunol. Methods 243, 155 166.
6. Chignola R, Pasti M, Candiani C, Franceschi A, Anselmi C, Tridente G et al. (1995) Escape mechanisms of human leukemic cells to long-term immunotoxin treatment in an in vitro experimental model. Int. J. Cancer 61, 535 541.
7. Crouch S, Kozlowski R, Slater K, Fletcher J (1993) The use of ATP bioluminescence as a measure of proliferation and cytotoxicity. J. Immunol. Methods 160, 81 88.
8. Xia M, Huang R, Witt KL, Southall N, Fostel J, Cho MH et al. (2008) Compound cytotoxicity profiling using quantitative high-throughput screening. Environ. Health Perspect. 116, 284 291.
9. O'Brien J, Wilson I, Orton T, Pognan F (2000) Investigation of the alamar blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur. J. Biochem. 267, 5421 5426.
10. Naciri M, Kuystermans D, Al-Rubeai M (2008) Monitoring pH and dissolved oxygen in mammalian cell culture using optical sensors. Cytotechnology 75, 245 250.
11. Strianese M, Zauner G, Tepper AW, Bubacco L, Breukink E, Aartsma TJ et al. (2009) A protein-based oxygen biosensor for high-throughput monitoring of cell growth and cell viability. Anal. Biochem. 385, 242 248.
12. Wodnicka M, Guarino R, Hemperly J, Timmins M, Stitt D, Bruce Pitner J (2000) Novel fluorescent technology platform for high throughput cytotoxicity and proliferation assays. J. Biomol. Screen. 5, 141 152.
13. Tomelleri C, Milotti E, Dalla Pellegrina C, Perbellini O, Del Fabbro A, Scupoli MT et al. (2008) A quantitative study of growth variability of tumour cell clones in vitro. Cell Prolif. 41, 177 191.
14. Parsons JD (2007) A high-throughput method for fitting dose-response curves using Microsoft Excel. Anal. Biochem. 360, 309 311.
15. Borchmann P, Hübel K, Schnell R, Engert A (1997) Idarubicin: a brief review on pharmacology and clinical use. Int. J. Clin. Pharmacol. Ther. 35, 80 83.
16. Hajek R, Vorliceck J, Slavik M (1996) Paclitaxel (taxol): a review of its antitumour activity in clinical studies. Neoplasma 43, 141 154.
17. Molad Y (2002) Update on colchicine and its mechanism of action. Curr. Rheumatol. Rep. 4, 252 256.
18. He L, Huang Yang CP, Horwitz SB (2001) Mutations in β-tubulin map to domains involved in regulation of microtubule stability in epothilone-resistant cell lines. Mol. Cancer Ther. 1, 3 10.
19. Toffoli G, Corona G, Simone F, Gigante M, De Angeli S, Boiocchi M (1996) Cellular pharmacology of idarubicinol in multidrug-resistant LoVo cells. Int. J. Cancer 67, 129 137.
20. Vial JP, Belloc F, Dumain P, Besnard S, Lacombe F, Boisseau MR et al. (1997) Study of the apoptosis induced in vitro by antitumoral drugs on leukaemic cells. Leuk. Res. 21, 163 172.
21. Holmes DT, Buhr KA (2007) Error propagation in calculated ratios. Clin. Biochem. 40, 728 734.