NHE3 function and phosphorylation are regulated by a calyculin A-sensitive phosphatase

American Journal of Physiology - Renal Physiology

Volumn 298, Issue 3, 2010, Pages

  Dynia, Diane W ^a   Steinmetz, Amy G ^a   Kocinsky, Hetal S ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Protein phosphatase 1; Protein phosphatase 2A

Indexed keywords

CALYCULIN A; OKADAIC ACID; PHOSPHOPROTEIN PHOSPHATASE 1; PHOSPHOPROTEIN PHOSPHATASE 2A; SODIUM PROTON EXCHANGE PROTEIN 3;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRUSH BORDER VESICLE; CELL MEMBRANE TRANSPORT; CELL STRAIN COS7; CONTROLLED STUDY; DEPHOSPHORYLATION; ENZYME ACTIVE SITE; GEL MOBILITY SHIFT ASSAY; KIDNEY CORTEX; KIDNEY TUBULE EPITHELIUM; MALE; NONHUMAN; OPOSSUM; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; RAT; TRANSPORT KINETICS;

EID: 77649169726     PISSN: 1931857X     EISSN: 15221466     Source Type: Journal    
DOI: 10.1152/ajprenal.00182.2009     Document Type: Article

References (47)

1. + gradient the sugar transport. J Membr Biol 42: 81-98, 1978.
2. Becchetti A, Malik B, Yue G, Duchatelle P, Al-Khalili O, Kleyman TR, Eaton DC. Phosphatase inhibitors increase the open probability of ENaC in A6 cells. Am J Physiol Renal Physiol 283: F1030-F1045, 2002.
3. Becker W, Kentrup H, Klumpp S, Schultz JE, Joost HG. Molecular cloning of a protein serine/threonine phosphatase containing a putative regulatory tetratricopeptide repeat domain. J Biol Chem 269: 22586-22592, 1994.
4. Biemesderfer D, Rutherford PA, Nagy T, Pizzonia JH, Abu-Alfa AK, Aronson PS. Monoclonal antibodies for high-resolution localization of NHE3 in adult and neonatal rat kidney. Am J Physiol Renal Physiol 273: F289-F299, 1997.
5. Ceulemans H, Bollen M. Functional diversity of protein phosphatase-1, a cellular economizer and reset button. Physiol Rev 84: 1-39, 2004.
6. Ceulemans H, Stalmans W, Bollen M. Regulator-driven functional diversification of protein phosphatase-1 in eukaryotic evolution. Bioessays 24: 371-381, 2002.
7. Chen W, Possemato R, Campbell KT, Plattner CA, Pallas DC, Hahn WC. Identification of specific PP2A complexes involved in human cell transformation. Cancer Cell 5: 127-136, 2004.
8. Darman RB, Flemmer A, Forbush B. Modulation of ion transport by direct targeting of protein phosphatase type 1 to the Na-K-Cl cotransporter. J Biol Chem 276: 34359-34362, 2001.
9. Donowitz M, Li X. Regulatory binding partners and complexes of NHE3. Physiol Rev 87: 825-872, 2007.
10. Flemmer AW, Gimenez I, Dowd BF, Darman RB, Forbush B. Activation of the Na-K-Cl cotransporter NKCC1 detected with a phosphospecific antibody. J Biol Chem 277: 37551-37558, 2002.
11. Gergs U, Boknik P, Buchwalow I, Fabritz L, Matus M, Justus I, Hanske G, Schmitz W, Neumann J. Overexpression of the catalytic subunit of protein phosphatase 2A impairs cardiac function. J Biol Chem 279: 40827-40834, 2004.
12. Gupta V, Ogawa AK, Du X, Houk KN, Armstrong RW. A model for binding of structurally diverse natural product inhibitors of protein phosphatases PP1 and PP2A. J Med Chem 40: 3199-3206, 1997.
13. Hinds TD Jr, Sanchez ER. Protein phosphatase 5. Int J Biochem Cell Biol 40: 2358-2362, 2008.
14. Huang HS, Pozarowski P, Gao Y, Darzynkiewicz Z, Lee EY. Protein phosphatase-1 inhibitor-3 is co-localized to the nucleoli and centrosomes with PP1gamma1 and PP1alpha, respectively. Arch Biochem Biophys 443: 33-44, 2005.
15. Huang X, Honkanen RE. Molecular cloning, expression, and characterization of a novel human serine/threonine protein phosphatase, PP7, that is homologous to Drosophila retinal degeneration C gene product (rdgC). J Biol Chem 273: 1462-1468, 1998.
16. i cotransporter is a phosphoprotein. Kidney Blood Press Res 24: 1-4, 2001.
17. Janssens V, Goris J. Protein phosphatase 2A: a highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling. Biochem J 353: 417-439, 2001.
18. Khew-Goodall Y, Hemmings BA. Tissue-specific expression of mRNAs encoding alpha- and beta-catalytic subunits of protein phosphatase 2A. FEBS Lett 238: 265-268, 1988.
19. C with alpha4 protein. Protein Expr Purif 31: 19-33, 2003.
20. Kocinsky HS, Dynia DW, Wang T, Aronson PS. NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity. Am J Physiol Renal Physiol 293: F212-F218, 2007.
21. + exchanger NHE3 at PKA consensus sites. Am J Physiol Renal Physiol 289: F249-F258, 2005.
22. + channel in cultured opossum proximal tubule cells by protein phosphatases 1 and 2A. Jpn J Physiol 50: 249-256, 2000.
23. + exchanger NHE3 isoform is regulated by the phosphatidylinositol 3-kinase pathway. J Biol Chem 273: 20828-20836, 1998.
24. + exchanger NHE3 activity by cAMP-dependent protein kinase phosphorylation-dependent and -independent mechanisms. J Biol Chem 272: 28672-28679, 1997.
25. + exchanger but not in mice lacking NHE2. Am J Physiol Renal Physiol 281: F718-F727, 2001.
26. + exchanger isoform NHE3 are involved in stimulation and inhibition by protein kinases/growth factors. J Biol Chem 270: 13716-13725, 1995.
27. +-ATPase. Am J Physiol Renal Fluid Electrolyte Physiol 269: F673-F680, 1995.
28. Lorenz JN, Schultheis PJ, Traynor T, Shull GE, Schnermann J. Micropuncture analysis of single-nephron function in NHE3-deficient mice. Am J Physiol Renal Physiol 277: F447-F453, 1999.
29. Marin P, Nastiuk KL, Daniel N, Girault JA, Czernik AJ, Glowinski J, Nairn AC, Premont J. Glutamate-dependent phosphorylation of elongation factor-2 and inhibition of protein synthesis in neurons. J Neurosci 17: 3445-3454, 1997.
30. Moe OW. Acute regulation of proximal tubule apical membrane Na/H exchanger NHE-3: role of phosphorylation, protein trafficking, and regulatory factors. J Am Soc Nephrol 10: 2412-2425, 1999.
31. Moe OW, Amemiya M, Yamaji Y. Activation of protein kinase A acutely inhibits and phosphorylates Na/H exchanger NHE-3. J Clin Invest 96: 2187-2194, 1995.
32. r 100,000 substrate for calmodulin-dependent protein kinase III in mammalian cells as elongation factor-2. J Biol Chem 262: 17299-17303, 1987.
33. Oliver CJ, Shenolikar S. Physiologic importance of protein phosphatase inhibitors. Front Biosci 3: D961-D972, 1998.
34. Prickett TD, Brautigan DL. The alpha4 regulatory subunit exerts opposing allosteric effects on protein phosphatases PP6 and PP2A. J Biol Chem 281: 30503-30511, 2006.
35. Redpath NT, Proud CG. Activity of protein phosphatases against initiation factor-2 and elongation factor-2. Biochem J 272: 175-180, 1990.
36. Redpath NT, Proud CG. Differing effects of the protein phosphatase inhibitors okadaic acid and microcystin on translation in reticulocyte lysates. Biochim Biophys Acta 1093: 36-41, 1991.
37. + exchanger 3 (NHE3) and stimulates NHE3 basal activity by phosphorylating a separate site in NHE3. Mol Biol Cell 19: 3859-3870, 2008.
38. + exchanger. Nat Genet 19: 282-285, 1998.
39. Strack S, Kini S, Ebner FF, Wadzinski BE, Colbran RJ. Differential cellular and subcellular localization of protein phosphatase 1 isoforms in brain. J Comp Neurol 413: 373-384, 1999.
40. Swingle M, Ni L, Honkanen RE. Small-molecule inhibitors of Ser/Thr protein phosphatases: specificity, use and common forms of abuse. Methods Mol Biol 365: 23-38, 2007.
41. Valenti G, Procino G, Carmosino M, Frigeri A, Mannucci R, Nicoletti I, Svelto M. The phosphatase inhibitor okadaic acid induces AQP2 translocation independently from AQP2 phosphorylation in renal collecting duct cells. J Cell Sci 113: 1985-1992, 2000.
42. Wang D, Sun H, Lang F, Yun CC. Activation of NHE3 by dexamethasone requires phosphorylation of NHE3 at Ser663 by SGK1. Am J Physiol Cell Physiol 289: C802-C810, 2005.
43. Wang T, Yang CL, Abbiati T, Schultheis PJ, Shull GE, Giebisch G, Aronson PS. Mechanism of proximal tubule bicarbonate absorption in NHE3 null mice. Am J Physiol Renal Physiol 277: F298-F302, 1999.
44. Wiederkehr MR, Zhao H, Moe OW. Acute regulation of Na/H exchanger NHE3 activity by protein kinase C: role of NHE3 phosphorylation. Am J Physiol Cell Physiol 276: C1205-C1217, 1999.
45. Yu P, Asico LD, Eisner GM, Hopfer U, Felder RA, Jose PA. Renal protein phosphatase 2A activity and spontaneous hypertension in rats. Hypertension 36: 1053-1058, 2000.
46. Zhang Y, Lin DH, Wang ZJ, Jin Y, Yang B, Wang WH. K restriction inhibits protein phosphatase 2B (PP2B) and suppression of PP2B decreases ROMK channel activity in the CCD. Am J Physiol Cell Physiol 294: C765-C773, 2008.
47. Zhao H, Wiederkehr MR, Fan L, Collazo RL, Crowder LA, Moe OW. Acute inhibition of Na/H exchanger NHE-3 by cAMP. Role of protein kinase a and NHE-3 phosphoserines 552 and 605. J Biol Chem 274: 3978-3987, 1999.