Analysis of Wnt pathway genes during ex vivo expansion and neutrophil differentiation of umbilical cord-blood derived CD34+ cells

Vox Sanguinis

Volumn 98, Issue 3 A, 2010, Pages

  Gallagher, Ronald C J ^a   Tura Ceide, O ^a   Turner, M ^a   Barclay, R ^a  

^a SCOTTISH NATIONAL BLOOD TRANSFUSION SERVICE   (United Kingdom)

Author keywords

CD34+ stem cell; Differentiation; Neutrophil; QPCR; WNT genes

Indexed keywords

WNT PROTEIN;

ARTICLE; CD34 SELECTION; CELL DIFFERENTIATION; CELL EXPANSION; CORD BLOOD STEM CELL TRANSPLANTATION; DOWN REGULATION; EX VIVO STUDY; GENE EXPRESSION; GENETIC ANALYSIS; HUMAN; HUMAN CELL; MALE; NEUTROPHIL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; UMBILICAL CORD BLOOD; UPREGULATION;

ANTIGENS, CD34; CELL DIFFERENTIATION; CELLS, CULTURED; DNA, COMPLEMENTARY; FETAL BLOOD; FRIZZLED RECEPTORS; GENE EXPRESSION PROFILING; HEMATOPOIETIC CELL GROWTH FACTORS; HEMATOPOIETIC STEM CELLS; HUMANS; INFANT, NEWBORN; NEUTROPHILS; POLYMERASE CHAIN REACTION; RNA, MESSENGER; TRANSCRIPTION, GENETIC; WNT PROTEINS;

EID: 77449135592     PISSN: 00429007     EISSN: 14230410     Source Type: Journal    
DOI: 10.1111/j.1423-0410.2009.01299.x     Document Type: Article

References (18)

1. Roose J, Clevers H : TCF transcription factors: molecular switches in carcinogenesis. Biochim Biophys Acta 1999 1424 : M23 M37
2. van Es JH, Barker N, Clevers H : You Wnt some, you lose some: oncogenes in the Wnt signaling pathway. Curr Opin Genet Dev 2003 13 : 28 33
3. Walston T, Tuskey C, Edgar L, Hawkins N, Ellis G, Bowerman B, Wood W, Hardin J : Multiple Wnt signaling pathways converge to orient the mitotic spindle in early C. elegans embryos. Dev Cell 2004 7 : 831 841
4. Austin TW, Solar GP, Ziegler FC, Liem L, Matthews W : A role for the Wnt gene family in hematopoiesis: expansion of multilineage progenitor cells. Blood 1997 89 : 3624 3635
5. Murdoch B, Chadwick K, Martin M, Shojaei F, Shah KV, Gallacher L, Moon RT, Bhatia M : Wnt-5A augments repopulating capacity and primitive hematopoietic development of human blood stem cells in vivo. Proc Natl Acad Sci U S A 2003 100 : 3422 3427
6. Reya T, Duncan AW, Ailles L, Domen J, Scherer DC, Willert K, Hintz L, Nusse R, Weissman IL : A role for Wnt signalling in self-renewal of haematopoietic stem cells. Nature 2003 423 : 409 414
7. Kirstetter P, Anderson K, Porse BT, Jacobsen SE, Nerlov C : Activation of the canonical Wnt pathway leads to loss of hematopoietic stem cell repopulation and multilineage differentiation block. Nat Immunol 2006 7 : 1048 1056
8. Scheller M, Huelsken J, Rosenbauer F, Taketo MM, Birchmeier W, Tenen DG, Leutz A : Hematopoietic stem cell and multilineage defects generated by constitutive beta-catenin activation. Nat Immunol 2006 7 : 1037 1047
9. Tura O, Barclay GR, Roddie H, Davies J, Turner ML : Optimal ex vivo expansion of neutrophils from PBSC CD34+ cells by a combination of SCF, Flt3-L and G-CSF and its inhibition by further addition of TPO. J Transl Med 2007 5 : 53
10. Gupta R, Hong D, Iborra F, Sarno S, Enver T : NOV (CCN3) functions as a regulator of human hematopoietic stem or progenitor cells. Science 2007 316 : 590 593
11. Linzmeier R, Michaelson D, Liu L, Ganz T : The structure of neutrophil defensin genes. FEBS Lett 1993 326 : 299 300
12. Skubitz KM, Campbell KD, Ahmed K, Skubitz AP : CD66 family members are associated with tyrosine kinase activity in human neutrophils. J Immunol 1995 155 : 5382 5390
13. Chambers SM, Boles NC, Lin KY, Tierney MP, Bowman TV, Bradfute SB, Chen AJ, Merchant AA, Sirin O, Weksberg DC, Merchant MG, Fisk CJ, Shaw CA, Goodell MA : Hematopoietic fingerprints: an expression database of stem cells and their progeny. Cell Stem Cell 2007 1 : 578 591
14. Wong CK, Luo W, Deng Y, Zou H, Ye Z, Lin SC : The DIX domain protein coiled-coil-DIX1 inhibits c-Jun N-terminal kinase activation by Axin and dishevelled through distinct mechanisms. J Biol Chem 2004 279 : 39366 39373
15. Friedman AD : Transcriptional regulation of granulocyte and monocyte development. Oncogene 2002 21 : 3377 3390
16. Gallagher RC, Hay T, Meniel V, Naughton C, Anderson TJ, Shibata H, Ito M, Clevers H, Noda T, Sansom OJ, Mason JO, Clarke AR : Inactivation of Apc perturbs mammary development, but only directly results in acanthoma in the context of Tcf-1 deficiency. Oncogene 2002 21 : 6446 6457
17. Baik I, Devito WJ, Ballen K, Becker PS, Okulicz W, Liu Q, Delpapa E, Lagiou P, Sturgeon S, Trichopoulos D, Quesenberry PJ, Hsieh CC : Association of fetal hormone levels with stem cell potential: evidence for early life roots of human cancer. Cancer Res 2005 65 : 358 363
18. Li Q, Cai H, Liu Q, Tan WS : Differential gene expression of human CD34+ hematopoietic stem and progenitor cells before and after culture. Biotechnol Lett 2006 28 : 389 394