Coupling cytotoxicity biomarkers with DNA damage assessment in TK6 human lymphoblast cells

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 696, Issue 2, 2010, Pages 167-178

  Shi, Jing ^a   Springer, Sandra ^a   Escobar, Patricia ^a  

^a BioReliance Corporation   (United States)

Author keywords

Apoptosis; Biomarkers; Comet assay; Cytotoxicity; DNA damage; Genotoxicity testing

Indexed keywords

2,4 DICHLOROPHENOL; 4 NITROQUINOLINE 1 OXIDE; ADENOSINE TRIPHOSPHATE; ALCOHOL; BIOLOGICAL MARKER; CASPASE 3; CASPASE 7; ETOPOSIDE; GLUCOSE; MESYLIC ACID ETHYL ESTER; MESYLIC ACID METHYL ESTER; TRYPAN BLUE; TUNICAMYCIN;

ARTICLE; CELL COUNT; COMET ASSAY; CONTROLLED STUDY; CYTOTOXICITY; DNA DAMAGE; ENZYME ACTIVATION; GENOTOXICITY; HUMAN; HUMAN CELL; IN VITRO STUDY; LYMPHOBLAST; PRIORITY JOURNAL;

ACUTE TOXICITY TESTS; ADENOSINE TRIPHOSPHATE; APOPTOSIS; BIOLOGICAL MARKERS; CASPASE 3; CASPASE 7; CELL LINE; COMET ASSAY; DNA DAMAGE; HUMANS; LYMPHOCYTES; MUTAGENS;

EID: 77249135763     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2010.01.008     Document Type: Article

References (35)

1. ICH. Topic S2A. Genotoxicity: guidance on specific aspects of regulatory genotoxicity tests for pharmaceuticals. Proceedings of the International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (1995), Geneva
2. OECD. Guideline for the Testing of Chemicals. No. 473. In Vitro Mammalian Chromosome Aberration Test (1997), Organisation for Economic Cooperation and Development, Paris
3. OECD. Guideline for the Testing of Chemicals. No. 476. In Vitro Mammalian Cell Gene Mutation Test (1997), Organisation for Economic Cooperation and Development, Paris
4. Fellows M.D., and O'Donovan M.R. Cytotoxicity in cultured mammalian cells is a function of the method used to estimate it. Mutagenesis 22 (2007) 275-280
5. Crouch S., Kozlowski R., Slater K., and Fletcher J. The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity. J. Immunol. Methods 160 (1993) 81-88
6. Kricka L.J. Clinical and biochemical applications of luciferases and luciferins. Anal. Biochem. 175 (1988) 14-21
7. Riss T., Moravec R., and Niles A. Selecting cell-based assays for drug discovery screening. Cell Notes 13 (2005) 16-21
8. Huang R., Southall N., Cho M.H., Xia M., Inglese J., and Austin C.P. Characterization of diversity in toxicity mechanism using in vitro cytotoxicity assays in quantitative high throughput screening. Chem. Res. Toxicol. 21 (2008) 659-667
9. Uckun F.M., Dibirdik I., Qazi S., Vassilev A., Ma H., Mao C., Benyumov A., and Emami K.H. Anti-breast cancer activity of LFM-A13, a potent inhibitor of Polo-like kinase (PLK). Bioorg. Med. Chem. 15 (2007) 800-814
10. Xia M., Huang R., Witt K.L., Southall N., Fostel J., Cho M.-H., Jadhav A., Smith C.S., Inglese J., Portier C.J., Tice R.R., and Austin C.P. Compound cytotoxicity profiling using quantitative high-throughput screening. Environ. Health Perspect. 116 (2008) 284-291
11. Armstrong M.J., Bean C.L., and Gallow S.M. A quantitative assessment of the cytotoxicity associated with chromosomal aberration detection in Chinese hamster ovary cells. Mutat. Res. 265 (1992) 45-60
12. Elia M.C., Storer R.D., Harmon L.S., Kraynak A.R., McKelvey T.W., Hertzog P.R., Keenan K.P., DeLuca J.G., and Nichols W.W. Cytotoxicity as measured by trypan blue as a potentially confounding variable in the in vitro alkaline elution/rat hepatocyte assay. Mutat. Res. 291 (1993) 193-205
13. Elia M.C., Storer R.D., McKelvey T.W., Kraynak A.R., Barnum J.E., Harmon L.S., DeLuca J.G., and Nichols W.W. Rapid DNA degradation in primary rat hepatocytes treated with diverse cytotoxic chemicals: analysis by pulsed field gel electrophoresis and implications for alkaline elution assays. Environ. Mol. Mutagen. 24 (1994) 181-191
14. Storer R.D., KcKelvey T.W., Kraynak A.R., Elia M.C., Barnum J.E., Harmon L.S., Nichols W.W., and DeLuca J.G. Revalidation of the in vitro alkaline elution/rat hepatocyte assay for DNA damage: improved criteria for assessment of cytotoxicity and genotoxicity and results for 81 compounds. Mutat. Res. 368 (1996) 59-101
15. Thornberry N.A. Caspases: key mediators of apoptosis. Chem. Biol. 5 (1998) 97-103
16. Bayascas J.R., Yuste V.J., Benito E., Garcia-Fernàndez J., and Comella J.X. Isolation of AmphiCASP-3/7, an ancestral caspase from amphioxus (Branchiostoma floridae). Evolutionary considerations for vertebrate caspases. Cell Death Differ. 9 (2002) 1078-1089
17. Le D.A., Wu Y., Huang Z., Matsushita K., Plesnila N., Augustinack J.C., Hyman B.T., Yuan J., Kuida K., Flavell R.A., and Moskowitz M.A. Caspase activation and neuroprotection in caspase-3-deficient mice after in vivo cerebral ischemia and in vitro oxygen glucose deprivation. Proc. Natl. Acad. Sci. U.S.A. 99 (2002) 15188-15193
18. Liu D., Li C., Chen Y., Burnett C., Liu X.Y., Downs S., Collins R.D., and Hawiger J. Nuclear import of proinflammatory transcription factors is required for massive liver apoptosis induced by bacterial lipopolysaccharide. J. Biol. Chem. 279 (2004) 48434-48442
19. Ren Y.G., Wagner K.W., Knee D.A., Aza-Blanc P., Nasoff M., and Deveraux Q.L. Differential regulation of the TRAIL death receptors DR4 and DR5 by the signal recognition particle. Mol. Biol. Cell. 15 (2004) 5064-5074
20. Singh N.P., McCoy M.T., Tice R.R., and Schneider E.L. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 175 (1998) 184-191
21. Tice R.R., Agurell E., Anderson D., Burlinson B., Hartmann A., Kobayashi H., Miyamae Y., Rojas E., Ryu J.-C., and Sasaki Y. Single cell gel/Comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen. 35 (2000) 206-221
22. Henderson L., Wolfreys A., Fedyk J., Bourner C., and Windebank S. The ability of the Comet assay to discriminate between genotoxins and cytotoxins. Mutagenesis 13 (1998) 89-94
23. Escobar P.A., Olivero O.A., Wade N.A., Abrams E.J., Nesel C.J., Ness R.B., Day R.D., Day B.W., Meng Q., O'Neill J.P., Walker D.M., Poirier M.C., Walker V.E., and Bigbee W.L. Genotoxicity assessed by the comet and GPA assays following in vitro exposure of human lymphoblastoid cells (H9) or perinatal exposure of mother-child pairs to AZT or AZT-3TC. Environ. Mol. Mutagen. 48 (2007) 330-343
24. Shi J., Bezabhie R., and Szkudlinska A. Further evaluation of a flow cytometric in vitro micronucleus assay in CHO-K1 cells: a reliable platform that detects micronuclei and discriminates apoptotic bodies. Mutagenesis 25 (2010) 33-40
25. Fritsch R.M., Schneider G., Saur D., Scheibel M., and Schmid R.M. Translational repression of MCL-1 couples stress-induced eIF2α phosphorylation to mitochondrial apoptosis initiation. J. Biol. Chem. 282 (2007) 22551-22562
26. Hastwell P.W., Chai L.-L., Roberts K.J., Webster T.W., Harvey J.S., Rees R.W., and Walmsley R.M. High-specificity and high-sensitivity genotoxicity assessment in a human cell line: validation of the GreenScreen HC GADD45a-GFP genotoxicity assay. Mutat. Res. 697 (2006) 160-175
27. Lorge E., Hayashi M., Albertini S., and Kirkland D. Comparison of different methods for an accurate assessment of cytotoxicity in the in vitro micronucleus test. I. Theoretical aspects. Mutat. Res. 655 (2008) 1-3
28. Fellows M.D., O'Donovan M.R., Lorge E., and Kirkland D. Comparison of different methods for an accurate assessment of cytotoxicity in the in vitro micronucleus test. II. Practical aspects with toxic agents. Mutat. Res. 655 (2008) 4-21
29. Dahan-Grobgeld E., Livneh Z., Maretzek A.F., Polak-Charcon S., Eichenbaum Z., and Degani H. Reversible induction of ATP synthesis by DNA damage and repair in Escherichia coli in vivo NMR studies. J. Biol. Chem. 273 (1998) 30232-30238
30. 13C-NMR studies. Biochim. Biophys. Acta 1052 (1990) 255-263
31. 31P NMR spectroscopy. Biochem. Biophys. Res. Commun. 183 (1992) 114-120
32. Kwok J.B.J., and Tattersall M.H.N. DNA fragmentation, dATP pool elevation and potentiation of antifolate cytotoxicity in L1210 cells by hypoxanthine. Br. J. Cancer 65 (1992) 503-508
33. Nobel C., Stefan I., Burgess D.H., Zhivotovsky B., Burkitt M.J., Orrenius S., and Andrew F.G. Mechanism of dithiocarbamate inhibition of apoptosis: thiol oxidation by dithiocarbamate disulfides directly inhibits processing of the caspase-3 proenzyme. Chem. Res. Toxicol. 10 (1997) 636-643
34. Hartman A., and Speit G. The contribution of cytotoxicity to DNA-effects in the single cell gel test (Comet assay). Toxicol. Lett. 90 (1997) 183-188
35. Collins A.R., Oscoz A.A., Brunborg G., Gaivao I., Giovannelli L., Kruszewski M., Smith C.C., and Stetina R. The comet assay: topical issues. Mutagenesis 23 (2008) 143-151