A CE-based assay for human protein kinase CK2 activity measurement and inhibitor screening

Electrophoresis

Volumn 31, Issue 4, 2010, Pages 634-640

  Gratz, Andreas ^a   Götz, Claudia ^b   Jose, Joachim ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b SAARLAND UNIVERSITY   (Germany)

Author keywords

CE; CK2; Kinase inhibitor; Mobility shift; Protein kinase

Indexed keywords

ELECTROLYTES; ENZYMES; NAPHTHALENE; PEPTIDES;

ACTIVITY MEASUREMENTS; CE; HUMAN PROTEINS; INHIBITOR SCREENINGS; KINASE INHIBITORS; MOBILITY SHIFT; PHOSPHORYLATED SUBSTRATES; PROTEIN KINASE; PROTEIN KINASE CK2; SUBSTRATE PEPTIDES;

PHOSPHORYLATION;

4,5,6,7 TETRABROMOBENZOTRIAZOLE; 5 [(2 AMINOETHYL)AMINO]NAPHTHALENE 1 SULFONIC ACID; BENZOTRIAZOLE DERIVATIVE; CYCLIN DEPENDENT KINASE 2; CYCLIN DEPENDENT KINASE 2 INHIBITOR; EMODIN; HOLOENZYME; NAPHTHALENE DERIVATIVE; RECOMBINANT ENZYME; SULFONIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; CAPILLARY ELECTROPHORESIS; CAPILLARY ZONE ELECTROPHORESIS; CARBOXY TERMINAL SEQUENCE; CONJUGATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME KINETICS; ENZYME SUBSTRATE; IC 50; MEASUREMENT; PHOSPHORYLATION; PROTEIN VARIANT; QUANTITATIVE ANALYSIS; RADIOMETRY; SCREENING; ULTRAVIOLET SPECTROSCOPY;

EID: 77149177961     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.200900514     Document Type: Article

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