Crystallization and preliminary X-ray crystallographic study of a 23S rRNA binding domain of the ribosomal protein L2 from Bacillus stearothermophilus

Journal of Structural Biology

Volumn 124, Issue 1, 1998, Pages 99-101

  Nakashima, Takashi ^a   Kimura, Makoto ^a   Nakagawa, Atsushi ^b   Tanaka, Isao ^b  

^a KYUSHU UNIVERSITY   (Japan)

^b HOKKAIDO UNIVERSITY   (Japan)

Author keywords

23S rRNA binding domain; Bacillus stearothermophilus; Crystallization; Ribosomal protein L2

Indexed keywords

BACTERIAL RNA; RIBOSOME PROTEIN; RIBOSOME RNA;

ARTICLE; CRYSTAL STRUCTURE; ESCHERICHIA COLI; GEOBACILLUS STEAROTHERMOPHILUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; STRUCTURE ANALYSIS; X RAY CRYSTALLOGRAPHY;

EID: 0032419766     PISSN: 10478477     EISSN: None     Source Type: Journal    
DOI: 10.1006/jsbi.1998.4053     Document Type: Article

References (15)

1. Beauclerk, A. A., and Cundliffe, E. (1988) The binding site for ribosomal protein L2 within 23S ribosomal RNA of Escherichia coli, EMBO J. 11, 3589-3594.
2. Egebjerg, J., Christiansen, J., and Garrett, R. A. (1991) Attachment sites of primary binding proteins L1, L2 and L23 on 23S ribosomal RNA of Escherichia coli, J. Mol. Biol. 222, 251-264.
3. Fahnestock, S. R. (1975) Evidence of the involvement of a 50S ribosomal protein in several active sites, Biochemistry 14, 5321-5327.
4. Gulle, H., Hoppe, E., Osswald, M., Greuer, B., Brimacombe, R., and Stoffler, G. (1988) RNA-protein cross-linking in Escherichia coli 50S ribosomal subunits: Determination of sites on 23S rRNA that are cross-linked to proteins L2, L4, L24 and L27 by 2-iminothiolane, Nucleic Acids Res. 16, 815-832.
5. Hample, H., Schulze, H., and Nierhaus, K. H., (1981) Ribosomal components from Escherichia coli 50S subunits involved in the reconstitution of peptidyltransferase activity, J. Biol. Chem. 256, 2284-2288.
6. Harada, N., Maemura, K., Yamasaki, N., and Kimura, M. (1998) Identification by site-directed mutagenesis of amino acid residues in ribosomal protein L2 that are essential for binding to 23S ribosomal RNA, Biochim. Biophys. Acta, in press.
7. Matthews, B. W. (1968) Solvent contents of protein crystals, J. Mol. Biol. 33, 491-497.
8. Nierhaus, K. H. (1980) Analysis of the assembly and function of the 50S subunit from Escherichia coli ribosomes by reconstitution, in Chambliss, G., et al. (Eds.), Ribosomes pp. 267-297, Univ. Park Press, Baltimore.
9. Otwinowski, Z. (1991) Oscillation data reduction program, in Wolf, W., Evans, P. R., and Leslie, A. G. W. (Eds.), Daresbury Study Weekend Proceedings, pp. 80-86, Science and Engineering Research Council, Daresbury Laboratory, UK.
10. Sakabe, N., Ikemizu, S., Sakabe, K., Higashi, T., Nakagawa, A., Watanabe, N., Adachi, S., and Sasaki, K. (1995) Weissenberg camera for macromolecules with imaging plate data collection system at the Photon Factory: Present status and future plan, Rev. Sci. Instrum. 66, 1276-1281.
11. Sakabe, N., Watanabe, N., Suzuki, M., Miyahara, J., Sasaki, K., and Sakabe, K. (1996) Imaging plate ST-V and large IP reader IPR4080, Acta Crystallogr. Sect. A, 52,
12. Schulze, H., and Nierhaus, K. H. (1982) Minimal set of ribosomal components for reconstitution of the peptidyl transferase activity, EMBO J. 1, 609-613.
13. Studier, F. W., Rosenberg, A. H., Dunn, J. J., and Dubendorff, J. W. (1990) Use of T7 RNA polymerase to direct expression of cloned gene, Methods Enzymol. 185, 60-89.
14. Sumpter, V. G., Tate, W. P., Nowotny, O., and Nierhaus, K. H. (1991) Modification of histidine residues on proteins from the 50S subunit of the Escherichia coli ribosome, Eur. J. Biochem. 196, 255-260.
15. Watanabe, K., and Kimura, M. (1985) Location of the binding region for 23S ribosomal RNA on ribosomal protein L2 from Bacillus stearothermophilus, Eur. J. Biochem. 153, 299-304.