Incomplete formation of intramolecular disulfide bond triggers degradation and aggregation of human consensus interferon-α mutant by Pichia pastoris

Applied Microbiology and Biotechnology

Volumn 85, Issue 6, 2010, Pages 1759-1767

  Wu, Dan ^a   Ma, Dong ^a   Hao, Yu You ^a   Chu, Ju ^a   Wang, Yong Hong ^a   Zhuang, Ying Ping ^a   Zhang, Si Liang ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Degradation and aggregation; Disulfide bond; Pichia pastoris; Recombinant human consensus interferon mutant

Indexed keywords

DISULFIDE BONDS; FERMENTATION BROTHS; IN-VITRO; INTRAMOLECULAR DISULFIDE BONDS; MERCAPTOETHANOL; NOVEL METHODS; PICHIA PASTORIS; SDS-PAGE; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS;

ANTIBIOTICS; BIOREACTORS; DIFFUSERS (OPTICAL); ELECTROPHORESIS; GLYCOPROTEINS; ISOMERS; MONOMERS; OLIGOMERS; SODIUM; SODIUM SULFATE;

DEGRADATION;

CONSENSUS INTERFERON; CONSENSUS INTERFERON ALPHA; DIMER; MERCAPTOETHANOL; MONOMER; UNCLASSIFIED DRUG;

BIOACTIVITY; BIODEGRADATION; BIOREACTOR; BIOTECHNOLOGY; ELECTROKINESIS; YEAST;

ARTICLE; BIOLOGICAL ACTIVITY; BIOREACTOR; BIOTRANSFORMATION; CONTROLLED STUDY; DISULFIDE BOND; FERMENTATION; IN VITRO STUDY; INCUBATION TIME; ISOMER; NONHUMAN; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN AGGREGATION; PROTEIN DEGRADATION;

BIOREACTORS; DISULFIDES; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; FERMENTATION; HUMANS; INTERFERON-ALPHA; MUTATION; PICHIA; PROTEIN STABILITY; RECOMBINANT PROTEINS;

PICHIA PASTORIS;

EID: 76849093775     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-2232-0     Document Type: Article

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