Purification, characterization, and cloning of a novel phytase with low pH optimum and strong proteolysis resistance from Aspergillus ficuum NTG-23

Bioresource Technology

Volumn 101, Issue 11, 2010, Pages 4125-4131

  Zhang, G Q ^a   Dong, X F ^a   Wang, Z H ^a   Zhang, Q ^a   Wang, H X ^b   Tong, J M ^a  

^a INSTITUTE OF PLANT PROTECTION   (China)

^b CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

Aspergillus ficuum; Cloning; Phytase; Purification

Indexed keywords

ANIMAL FEED SUPPLEMENTS; ASPERGILLUS FICUUM; DEAE CELLULOSE; GEL FILTRATION; ION-EXCHANGE CHROMATOGRAPHY; OPTIMAL TEMPERATURE; PH OPTIMA; PHYTASE; PHYTASE ACTIVITY; PHYTASES; SDS-PAGE; SUBSTRATE SELECTIVITY;

CALCIUM; CELLULOSE; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; CLONING; GELS; ION EXCHANGE; MANGANESE; MANGANESE COMPOUNDS; METAL IONS; PURIFICATION; SULFUR COMPOUNDS; ZINC;

METAL REFINING;

EDETIC ACID; PEPSIN A; PHYTASE; TRYPSIN;

CHROMATOGRAPHY; CLONE; EDTA; ENZYME; FILTRATION; INCUBATION; ION EXCHANGE; PH;

ACIDITY; ARTICLE; ASPERGILLUS; ASPERGILLUS FICUUM; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; FAST PROTEIN LIQUID CHROMATOGRAPHY; GEL FILTRATION; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DEGRADATION;

6-PHYTASE; AMINO ACID SEQUENCE; ASPERGILLUS; BASE SEQUENCE; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

CALCIUM; CELLULOSE; CHROMATOGRAPHY; CLONES; ENZYMES; ION EXCHANGE; MANGANESE COMPOUNDS; PURIFICATION; REFINING; SULFUR COMPOUNDS;

ANIMALIA; ASPERGILLUS FICUUM;

EID: 76749170185     PISSN: 09608524     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biortech.2010.01.001     Document Type: Article

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