Biochemical characterisation of extracellular phytase (myo-inositol hexakisphosphate phosphohydrolase) from a hyper-producing strain of Aspergillus niger van Teighem

Journal of Industrial Microbiology and Biotechnology

Volumn 32, Issue 4, 2005, Pages 141-147

  Vats, Purva ^a   Banerjee, U C ^b  

^a INSTITUTE OF MICROBIAL TECHNOLOGY   (India)

^b NATIONAL INSTITUTE OF PHARMACEUTICAL EDUCATION AND RESEARCH NIPER   (India)

Author keywords

Acid phosphatases; Aspergillus niger; Phytases

Indexed keywords

FUNGAL ENZYME; PHYTASE;

ARTICLE; ASPERGILLUS NIGER; CATALYSIS; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME PURIFICATION; ENZYME STABILITY; ENZYME STRUCTURE; ENZYME SYNTHESIS; FUNGAL STRAIN; FUNGUS ISOLATION; GEL FILTRATION CHROMATOGRAPHY; HYDROLYSIS; ION EXCHANGE CHROMATOGRAPHY; MICHAELIS CONSTANT; MOLECULAR WEIGHT; PH;

6-PHYTASE; ASPERGILLUS NIGER; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; INDUSTRIAL MICROBIOLOGY; KINETICS; PHYTIC ACID; TEMPERATURE; UP-REGULATION;

ASPERGILLUS NIGER;

EID: 20044364585     PISSN: 13675435     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10295-005-0214-5     Document Type: Article

References (39)

1. Bradford MM (1976) A rapid and sensitive method for quantitation of microgram amounts of protein using the principal of protein-dye binding. Anal Biochem 72:248-254
2. Chen CC, Hunag CT, Cheng KJ (2001) Improvement of phytase thermostability by using sorghum liquor wastes supplemented with starch. Biotechnol Lett 23:331-333
3. Common FH (1989) Biological availability of phosphorus for pigs. Nature 143:370-380
4. Ehrlich KC, Montalbano BG, Mullaney EJ, Dischinger HC, Ullah AHJ (1993) Identification and cloning of a second phytase gene (phyB) from Aspergillus niger (ficuum). Biochem Biophys Res Commun 195:53-57
5. Greiner R, Konietzny U (1996) Construction of a bioreactor to produce special breakdown products of phytate. J Biotechnol 48:153-159
6. Greiner R, Konietzny U, Jany KD (1993) Purification and characterization of two phytases from Escherichia coli. Arch Biochem Biophys 303:107-113
7. Greiner R, Haller E, Konietzny U, Jany KD (1997) Purification and characterization of a phytase from Klebsiella terrigena. Arch Biochem Biophys 341:201-206
8. Han YW, Lei XG (1999) Role of glycosylation in functional expression of an Aspergillus niger phytase (phyA) in Pichia pastoris. Arch Biochem Biophys 364:83-90
9. Heinonen JK, Lahti RJ (1981) A new and convenient colorimetric determination of inorganic orthophosphate and its application to the assay of inorganic pyrophosphatase. Anal Biochem 113:313-317
10. Iqbal TH, Lewis KO, Cooper BT (1994) Phytase activity in the human and rat small intestine. Gut 35:1233-1236
11. Kerovuo J, Lauraeus M, Nurminen P, Kalkkinen N, Apajalahti J (1998) Isolation, characterization, molecular gene cloning and sequencing of a novel phytase from Bacillus subtilis. Appl Environ Microbiol 64:2079-2085
12. Kerovuo J, Lappalainen I, Reinikainen T (2000) The metal dependence of Bacillus subrilis phytase. Biochem Biophys Res Commun 268:365-369
13. Kim YO, Kim HK, Bae KS, Yu JH, Oh TK (1998) Purification and properties of thermostable phytase from Bacillus sp. DS11. Enzyme Microb Technol 22:2-7
14. Kostrewa D, Wyss M, D'Arcy A, van Loon APGM (1999) Crystal structure of Aspergillus niger pH 2.5 optimum acid phosphatase at 2.4 Å resolution. J Mol Biol 288:965-974
15. Lammeli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
16. Maenz DD, Classen HL, (1998) Phytase activity in the small intestinal brush-border membrane of the chicken. Poult Sci 77:557-563
17. Mitchell DB, Vogel K, Weimann BJ, Pasamontes L, van Loon APGM (1997) The phytase subfamily of histidine acid phosphatase: Isolation of genes for two novel phytases from fungi Aspergillus terreus and Myceliophthora thermophila. Microbiology 143:245-252
18. Nagashima T, Tange T, Anazawa H (1999) Dephosphorylation of phytate by using the Aspergillus niger phytase with a high affinity for phytate. Appl Environ Microbiol 65:4682-4684
19. Nakamura Y, Fukuhara H, Sano L (2000) Secreted phytase activities of yeasts. Biosci Biotechnol Biochem 64:841-844
20. Sano L, Fukuhara H, Nakamura Y (1999) Phytase of the yeast Arxula adeninivorans. Biotechnol Lett 21:33-38
21. Segueilha L, Lambrechts C, Boze H, Moulin G, Galzy P (1992) Purification and properties of phytase from Schwanniomyces castellii. J Ferment Bioeng 74:7-11
22. Shah V, Parekh LJ (1990) Phytase from Klebsiella sp. PG-2-purification and properties. Indian J Biochem Biophys 27:98-102
23. Shimizu M (1992) Purification and characterization of phytase from Bacillus subtilis (natto) N-77. Biosci Biotechnol Biochem 56:1266-1269
24. Shimizu M (1993) Purification and characterization of phytase and acid phosphatase produced by Aspergillus oryzae K1. Biosci Biotechnol Biochem 57:1364-1365
25. Tambe SM, Kaklij GS, Kelkar SM, Parekh LJ (1994) Two distinct molecular forms of phytase from Klebsiella aerogenes: evidence for unusually small active enzyme peptide. J Ferment Bioeng 77:23-27
26. Ullah AHJ, Cummins BJ (1987) Purification, N-terminal amino acid sequence and characterisation of pH 2.5 optimum acid phosphatase (E.C.3.1.3.2) from Aspergillus ficuum. Prep Biochem 17:397-422
27. Ullah AHJ, Cummins BJ (1988) Aspergillus ficuum extracellular pH 6.0 optimum acid phosphatase: purification, N-terminal amino acid sequence and biochemical characterization. Prep Biochem 18:37-65
28. Ullah AHJ, Dischinger HC (1993) Identification of active site residues in Aspergillus ficuum extracellular pH 2.5 optimum acid phosphatase. Biochem Biophys Res Commun 192:754-759
29. Ullah AHJ, Gibson DM (1987) Extracellular phytase (E.C.3.1.3.8) from Aspergillus ficuum NRRL 3135: purification and characterization. Prep Biochem 17:63-91
30. Ullah AHJ, Phillippy BQ (1988) Immobilization of Aspergillus ficuum phytase: product characterization of the bioreactor. Prep Biochem 18:483-489
31. Ullah AHJ, Phillippy BQ (1994) Substrate selectivity in Aspergillus ficuum phytase and acid phosphatases using myoinositol phosphates. J Agric Food Chem 42:423-425
32. Ullah AHJ, Sethumadhavan K (1998a) Differences in the active site environment of Aspergillus ficuum phytases. Biochem Biophys Res Commun 243:458-462
33. Vats P, Banerjee UC (2002) Studies on the production of phytase by a newly isolated sp. of Aspergillus niger van teigham obtained from rotten wood-logs. Process Biochem 38:211-217
34. Vats P, Banerjee UC (2004) Production studies and catalytic properties of phytases (myo-inositol hexakis phosphate phosphor hydrolases): an overview. Enzyme Microb Technol 35:3-14
35. Vats P, Sahoo DK, Banerjee UC (2004) Production of phytase (myo-inositolhexakisphosphate phosphohydrolase) by Aspergillus niger van Teighem in laboratory-scale fermenter. Biotechnol Prog 20:737-743
36. Wodzinski RJ, Ullah AHJ (1996) Phytase. Adv Appl Microbiol 42:263-301
37. Wyss M, Pasamontes L, Friedlein A, Remy R, Tessier M, Kronenberger A, Middendorf A, Lehmann M, Schnoebelen L, Rothlisberger U, Kusznir E, Wahl G, Muller F, Lahm HW, Vogel K, Van Loon APGM (1999a) Biophysical characterization of fungal phytases (myo-inositolhexakisphosphate-phosphohydrolases): molecular size, glycosylation pattern and engineering of proteolytic resistance. Appl Environ Microbiol 65:359-366
38. Wyss M, Brugger R, Kronenberger A, Remy R, Fimbel R, Oesterhelt O, Lehmann M, Van Loon APGM (1999b) Biochemical characterization of fungal phytases (myo-inositolhexakisphosphate-phosphohydrolases): catalytic properties. Appl Environ Microbiol 65:367-373
39. Yoon SJ, Choi YJ, Min K, Cho KK, Kim JW, Lee SC, Jung YH (1996) Isolation and identification of phytase producing bacterium, Enlerobacter sp. 4 and enzymatic properties of phytase enzyme. Enzyme Microb Technol 18:449-454