GATEWAY™ technology and E. coli recombinant system produce a properly folded and functional recombinant allergen of the lipid transfer protein of apple (Mal d 3)

Protein Expression and Purification

Volumn 70, Issue 2, 2010, Pages 277-282

  Borges, Jean Philippe ^a   Culerrier, Raphaël ^a   Aldon, Didier ^a   Barre, Annick ^a   Benoist, Hervé ^b   Saurel, Olivier ^c   Milon, Alain ^c   Didier, Alain ^d   Rougé, Pierre ^a  

^a UNIVERSITÉ DE TOULOUSE   (France)

^b CHU RANGUEIL   (France)

^c CNRS   (France)

^d SERVICE DE PNEUMOLOGIE   (France)

Author keywords

Allergy; Gateway; Immunotherapy; Lipid transfer protein; Mal d 3; Structural analysis

Indexed keywords

ESCHERICHIA COLI; MALUS X DOMESTICA; PICHIA PASTORIS; RATTUS;

ALLERGEN; CARRIER PROTEIN; LIPID TRANSFER PROTEIN; MAL D 3 ALLERGEN; RECOMBINANT PROTEIN;

ANIMAL; APPLE; ARTICLE; CHEMISTRY; ESCHERICHIA COLI; GENETICS; HUMAN; METABOLISM; MOLECULAR CLONING; RAT;

ALLERGENS; ANIMALS; CARRIER PROTEINS; CLONING, MOLECULAR; ESCHERICHIA COLI; HUMANS; MALUS; RATS; RECOMBINANT PROTEINS;

EID: 76749144554     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.09.009     Document Type: Article

References (28)

1. Sanchez-Monge R., Lombardero M., Garcia-Selles F.J., Barber D., and Salcedo G. Lipid-transfer proteins are relevant allergens in fruit allergy. J. Allergy Clin. Immunol. 103 (1999) 514-519
2. Pastorello E.A., Farioli L., Pravettoni V., Ortolani C., Ispano M., Monza M., Baroglio C., Scibola E., Ansaloni R., Incorvaia C., and Conti A. The major allergen of peach (Prunus persica) is a lipid transfer protein. J. Allergy Clin. Immunol. 103 (1999) 520-526
3. Pastorello E.A., Pravettoni V., Farioli L., Ispano M., Fortunato D., Monza M., Giuffrida M.G., Rivolta F., Scibola E., Ansaloni R., Incorvaia C., Conti A., and Ortolani C. Clinical role of a lipid transfer protein that acts as a new apple-specific allergen. J. Allergy Clin. Immunol. 104 (1999) 1099-1106
4. Pastorello E.A., D'Ambrosio F.P., Pravettoni V., Farioli L., Giuffrida G., Monza M., Ansaloni R., Fortunato D., Scibola E., Rivolta F., Incorvaia C., Bengtsson A., Conti A., and Ortolani C. Evidence for a lipid transfer protein as the major allergen of apricot. J. Allergy Clin. Immunol. 105 (2000) 371-377
5. Pastorello E.A., Farioli L., Pravettoni V., Giuffrida M.G., Ortolani C., Fortunato D., Trambaioli C., Scibola E., Calamari A.M., Robino A.M., and Conti A. Characterization of the major allergen of plum as a lipid transfer protein. J. Chromatogr. B Biomed. Sci. Appl. 756 (2001) 95-103
6. Ballmer-Weber B.K. Lipid transfer protein as a potential panallergen?. Allergy 57 (2002) 873-875
7. van Ree R. Clinical importance of non-specific lipid transfer proteins as food allergens. Biochem. Soc. Trans. 30 (2002) 910-913
8. Salcedo G., Sanchez-Monge R., Diaz-Perales A., Garcia-Casado G., and Barber D. Plant non-specific lipid transfer proteins as food and pollen allergens. Clin. Exp. Allergy 34 (2004) 1336-1341
9. Asero R., Mistrello G., Roncarolo D., de Vries S.C., Gautier M.F., Ciurana C.L., Verbeek E., Mohammadi T., Knul-Brettlova V., Akkerdaas J.H., Bulder I., Aalberse R.C., and Van Ree R. Lipid transfer protein: a pan-allergen in plant-derived foods that is highly resistant to pepsin digestion. Int. Arch. Allergy Immunol. 124 (2001) 67-69
10. Asero R., Mistrello G., Roncarolo D., Amato S., and Falagiani P. Analysis of the heat stability of lipid transfer protein from apple. J. Allergy Clin. Immunol. 112 (2003) 1009-1011
11. Scheurer S., Lauer I., Foetisch K., San Miguel Moncin M., Retzek M., Hartz C., Enrique E., Lidholm J., Cistero-Bahima A., and Vieths S. Strong allergenicity of Pru av 3, the lipid transfer protein from cherry, is related to high stability against thermal processing and digestion. J. Allergy Clin. Immunol. 114 (2004) 900-907
12. 1H NMR data. A new folding for lipid carriers. Eur. J. Biochem. 226 (1994) 413-422
13. Fernández-Rivas M., González-Mancebo E., Rodríguez-Pérez R., Benito C., Sánchez-Monge R., Salcedo G., Alonso M.D., Rosado A., Tejedor M.A., Vila C., and Casas L. Clinically relevant peach allergy is related to peach lipid transfer protein, Pru p 3, in the Spanish population. J. Allergy Clin. Immunol. 112 (2003) 789-795
14. Díaz-Perales A., García-Casado G., Sánchez-Monge R., García-Selles F.J., Barber D., and Salcedo G. CDNA cloning and heterologous expression of the major allergens from peach and apple belonging to the lipid-transfer protein family. Clin. Exp. Allergy 32 (2002) 87-92
15. Díaz-Perales A., Sanz M.L., García-Casado G., Sánchez-Monge R., García-Selles F., Lombardero M., Polo F., Gamboa P.M., Barber D., and Salcedo G. Recombinant Pru p 3 and natural Pru p 3, a major peach allergen, show equivalent immunologic reactivity: a new tool for the diagnosis of fruit allergy. J. Allergy Clin. Immunol. 111 (2003) 628-633
16. Zuidmeer L., van Leeuwen W.A., Budde I.K., Cornelissen J., Bulder I., Rafalska I., Besoli N.T., Akkerdaas J.H., Asero R., Rivas M.F., Mancebo E.G., and van Ree R. Lipid transfer proteins from fruit, cloning, expression and quantification. Int. Arch. Allergy Immunol. 137 (2005) 273-281
17. Walhout A.J., Temple G.F., Brasch M.A., Hartley J.L., Lorson M.A., van den Heuvel S., and Vidal M. GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames of ORFeomes. Methods Enzymol. 328 (2002) 575-592
18. Borges J.-P., Jauneau A., Brulé C., Culerrier R., Barre A., Didier A., and Rougé P. The lipid transfer protein (LTP) essentially concentrate in the skin of Rosaceae fruits as a cell-surface exposed allergen. Plant Physiol. Biochem. 44 (2006) 535-542
19. Borges J.-P., Barre A., Culerrier R., Archimbaud N., Didier A., and Rougé P. How reliable is the structural prediction of IgE-binding epitopes of allergens: the case study of plant lipid transfer proteins. Biochimie 89 (2007) 83-91
20. Smith P.K., Krohn R.I., Hermanson G.T., Mallia A.K., Gartner F.H., Provenzano M.D., Fujimoto E.K., Goeke N.M., Olson B.J., and Klenk D.C. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150 (1987) 76-85
21. Tastet C., Lescuyer P., Diemer H., Luche S., van Dorsselaer A., and Rabilloud T. A versatile electrophoresis system for the analysis of high- and low-molecular-weight proteins. Electrophoresis 24 (2003) 1787-1794
22. Shevchenko A., Wilm M., Vorm O., Jensen O.N., Podtelejnikov A.V., Neubauer G., Shevchenko A., Mortensen P., and Mann M. A strategy for identifying gel-separated proteins in sequence databases by MS alone. Biochem. Soc. Trans. 24 (1996) 893-896
23. Sreerama N., Venyaminov S.Y., and Woody R.W. Estimation of the number of a-helical and b-strand segments in proteins using circular dichroism spectroscopy. Protein Sci. 8 (1999) 370-380
24. Liu M., Mao C., He C., Huang H., Nicholson J.K., and Lindon J. Improved WATERGATE pulse sequences for solvent suppression in NMR spectroscopy. J. Magn. Reson. 132 (1998) 125-129
25. Taudou G., Varin-Blank N., Jouin H., Marchand F., Weyer A., and Blank U. Expression of the alpha chain of human Fc epsilon RI in transfected rat basophilic leukemia cells: functional activation after sensitization with human mite-specific IgE. Int. Arch. Allergy Immunol. 100 (1993) 344-350
26. Pasquato N., Berni R., Folli C., Cianci M., Pantano S., Helliwell J.R., and Zanotti G. Crystal structure of peach Pru p 3, the prototypic member of the family of plant non-specific lipid transfer protein pan-allergens. J. Mol. Biol. 356 (2006) 684-694
27. Shin D.H., Lee J.Y., Hwang K.Y., Kim K.K., and Suh S.W. High-resolution crystal structure of the non-specific lipid-transfer protein from maize seedlings. Structure 3 (1995) 189-199
28. Borges J.-P., Culerrier R., Barre A., Granier C., Didier A., and Rougé P. Lipid transfer proteins from Rosaceae fruits share consensus epitopes responsible for their IgE-binding cross-reactivity. Biochem. Biophys. Res. Commun. 365 (2007) 685-690