New role of C-terminal 30 amino acids on the insoluble chitin hydrolysis in actively engineered chitinase from Vibrio parahaemolyticus

Applied Microbiology and Biotechnology

Volumn 76, Issue 1, 2007, Pages 123-133

  Chuang, Hsu Han ^a   Lin, Fu Pang ^a  

^a NATIONAL TAIWAN OCEAN UNIVERSITY   (Taiwan)

Author keywords

C terminal truncation; Chitinase; Crystalline chitin; Site directed mutagenesis; Vibrio parahaemolyticus

Indexed keywords

CHARACTERIZATION; CHITIN; DNA; ENZYMES; FLUORESCENCE SPECTROSCOPY; HYDROLYSIS;

C-TERMINAL TRUNCATION; CRYSTALLINE CHITIN; POLYMERASE CHAIN REACTION; SITE-DIRECTED MUTAGENESIS; VIBRIO PARAHAEMOLYTICUS;

AMINO ACIDS;

AMINO ACID; CHITIN; DNA FRAGMENT;

AMINO ACID; COLIFORM BACTERIUM; DNA; ENZYME ACTIVITY; FLUORESCENCE SPECTROSCOPY; GENE EXPRESSION; HYDROLYSIS; MUTAGENICITY; PHYSICOCHEMICAL PROPERTY; POLYMERASE CHAIN REACTION; POLYSACCHARIDE;

ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CHEMICAL ANALYSIS; CIRCULAR DICHROISM; ENZYME ENGINEERING; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; GENETIC CODE; KINETICS; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH MEASUREMENT; PHYSICAL CHEMISTRY; POLYMERASE CHAIN REACTION; PROTEIN FUNCTION; PROTEIN HYDROLYSIS; SPECTROFLUOROMETRY; TEMPERATURE; THERMOSTABILITY; VIBRIO PARAHAEMOLYTICUS;

BACTERIAL PROTEINS; BINDING SITES; CHITIN; CHITINASE; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; MODELS, MOLECULAR; MOLECULAR WEIGHT; MUTAGENESIS, SITE-DIRECTED; RECOMBINANT PROTEINS; STRUCTURE-ACTIVITY RELATIONSHIP; TEMPERATURE; VIBRIO PARAHAEMOLYTICUS;

ESCHERICHIA COLI; VIBRIO PARAHAEMOLYTICUS;

EID: 34547580195     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-007-0990-0     Document Type: Article

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