Molecular and biochemical characterization of an endochitinase (ChiA-HD73) from Bacillus thuringiensis subsp. kurstaki HD-73

Molecular Biotechnology

Volumn 39, Issue 1, 2008, Pages 29-37

  Barboza Corona, J Eleazar ^a   Reyes Rios, Dora M ^a,b   Salcedo Hernández, Rubén ^a   Bideshi, Dennis K ^c,d  

^a UNIVERSITY OF GUANAJUATO   (Mexico)

^b Secretaria de Educacion Publica   (Mexico)

^c CALIFORNIA BAPTIST UNIVERSITY   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Bacillus thuringiensis HD 73; Chitinases; Chitooligosaccharides; Escherichia coli

Indexed keywords

AMINES; BACTERIA; BACTERIOLOGY; ESCHERICHIA COLI; INSECT CONTROL; MODULAR CONSTRUCTION; PEST CONTROL;

BACILLUS THURINGIENSIS; BACILLUS THURINGIENSIS HD-73; BINDING DOMAINS; BIOCHEMICAL CHARACTERIZATIONS; BIOTECHNOLOGICAL APPLICATIONS; CATALYTIC DOMAINS; CHITINASE; CHITINASES; CHITOBIOSE; CHITOOLIGOSACCHARIDES; CLEAVAGE SITES; ENCODED PROTEINS; ENDOCHITINASE; ENZYMATIC ACTIVITIES; FLUOROGENIC SUBSTRATES; INSECTICIDAL CRYSTAL PROTEINS; ISOELECTRIC POINTS; MODULAR STRUCTURES; OPTIMAL TEMPERATURES; PH RANGES; PUTATIVE SIGNAL PEPTIDES; THURINGIENSIS;

SUBSTRATES;

CHITINASE; SIGNAL PEPTIDE; RECOMBINANT PROTEIN;

ARTICLE; BACILLUS; BACILLUS THURINGIENSIS KURSTAKI; BINDING AFFINITY; CATALYSIS; CHIA HD73 GENE; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE; GENE EXPRESSION; GENE SEQUENCE; GENOMICS; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; HYDROLYSIS; MOLECULAR BIOLOGY; MOLECULAR CLONING; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PROTEIN PURIFICATION; ZYMOGRAPHY; AMINO ACID SEQUENCE; BACILLUS THURINGIENSIS; CHEMISTRY; DNA SEQUENCE; ENZYME ACTIVE SITE; ENZYMOLOGY; GENETIC TRANSFORMATION; GENETICS; METABOLISM; MOLECULAR GENETICS; PH; PLASMID; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; TEMPERATURE;

BACILLUS THURINGIENSIS; BACILLUS THURINGIENSIS SEROVAR KURSTAKI; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; HEXAPODA; NEGIBACTERIA; POSIBACTERIA;

AMINO ACID SEQUENCE; BACILLUS THURINGIENSIS; CATALYTIC DOMAIN; CHITINASE; CLONING, MOLECULAR; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; TEMPERATURE; TRANSFORMATION, GENETIC;

EID: 52049099457     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-007-9025-4     Document Type: Article

References (34)

1. Muzarelly R. A. A., Jeuniaux C., & Gooday, G. W. (1986). Chitin in nature and technology (pp. 39-50). New York: Pergamon Press.
2. R. R. Granados Y. Fu B. Corsaro P. R. Hughes 2001 Enhancement of Bacillus thuringiensis toxicity to lepidopterous species with the enhancin from Trichoplusia ni granulovirus Biological Control 20 153 159
3. N. V. Shabrukova L. M. Shestakova D. R. Zainetdinova V. S. Gamayurova 2002 Research of acid hydrolysis of chitin-glucan and chitosan-glucan complexes Chemistry and Computational Simulation. Butlerov Communication 2 57 59
4. A. Regev M. Keller N. Strizhov B. Sneh E. Prudovski I. Chet I. Ginzberg Z. Koncz-Kalman Z. Konzcz J. Schell A. Zilberstein 1996 Synergistic activity of Bacillus thuringiensis δ-endotoxin and a bacterial endochitinase against Spodoptera littoralis Applied and Environmental Microbiology 62 3581 3586
5. A. Reyes-Ramírez B. I. Escudero-Abarca G. Aguilar-Uscanga P. M. Hayward-Jones J. E. Barboza-Corona 2004 Antifungal activity of Bacillus thuringiensis chitinase and its potential for the biocontrol of phytopathogenic fungi in soybean seeds Journal of Food Science 69 M131 134
6. L. de Morales la Vega J. E. Barboza-Corona M. G. Aguilar-Uscanga M. Ramírez-Lepe 2006 Purification and characterization of an exochitinase from Bacillus thuringiensis subsp. aizawai and its action against phytopathogenic fungi Canadian Journal of Microbiology 52 651 657
7. G. Casique-Arroyo D. Bideshi R. Salcedo-Hernández J. E. Barboza-Corona 2007 Development of a recombinant strain of Bacillus thuringiensis subsp. kurstaki HD-73 that produces the endochitinase ChiA74 Antonie van Leeuwenhoek 92 1 9
8. H. Höfte H. R. Whiteley 1989 Insecticidal crystal proteins of Bacillus thuringiensis Microbiological Reviews 53 242 255
9. L. Chernin Z. Ismailov S. Haran I. Chet 1995 Chitinolytic Enterobacter agglomerans antagonistic to fungal plant pathogens Applied and Environmental Microbiology 61 1720 1726
10. D. Bhattacharya A. Nagpure R. K. Gupta 2007 Bacterial chitinases: Properties and potential Critical Reviews in Biotechnology 27 21 28
11. T. Watanabe K. Kimura T. Sumiya N. Nikaidou K. Suzuki M. Suzuki M. Taiyoji S. Ferrer M Regue 1997 Genetic analysis of the chitinase system of Serratia marcescens 2170 Journal of Bacteriology 179 7111 7117
12. M. L. Wu Y. C. Chuang J. P. Chen C. S. Chen M. C. Chang 2001 Identification and characterization of the three chitin-binding domains with the multidomain chitinase Chi92 from Aeromonas hydrophila JP101 Applied and Environmental Microbiology 67 5100 5106
13. K. Susuki N. Sugawara M. Susuki T. Uchiyama F. Katouno N. Nikaidou T. Watanabe 2002 Chitinases A, B, and C1 of Serratia marcescens 2170 produced by recombinant Escherichia coli: Enzymatic properties and synergism on chitin degradation Bioscience, Biotechnology, and Biochemistry 66 1075 1083
14. S. Thamthiankul S. Suan-Ngay S. Tantimavanich W. Panbangred 2001 Chitinase from Bacillus thuringiensis subsp. pakistani Applied Microbiology and Biotechnology 56 395 401
15. J. E. Barboza-Corona E. Nieto-Mazzocco R. Velázquez-Robledo R. Salcedo-Hernández M. Bautista B. Jiménez J. E. Ibarra 2003 Cloning, sequencing, and expresión of the chitinase gene chiA74 from Bacillus thuringiensis Applied and Environmental Microbiology 69 1023 1029
16. W. F. Zhong L. H. Jiang W. Z. Yan P. Z Cai Z. X. Zhang Y. Pei 2003 Cloning and sequence of chitinase gene from Bacillus thuringiensis subsp. israeliensis Yi Chuan Xue Bao 30 364 369 (In Chinese)
17. N. Arora T. Ahmad R. Rajagopal R. K. Bhatnagar 2003 A constitutively expressed 36 kDa exochitinase from Bacillus thuringiensis HD-1 Biochemical and Biophysical Research Communications 307 620 625
18. Y. Lin G. Xiong 2004 Molecular cloning and sequence analysis of the chitinase gene from Bacillus thuringiensis serovar alesti Biotechnology Letters 26 635 639
19. F. Driss M. Kallassy-Awad N. Zouari S. Jaoua 2005 Molecular characterization of a novel chitinase from Bacillus thuringiensis subsp. kurstaki Journal of Applied Microbiology 99 945 953
20. D. Lereclus O. Arantes J. Chaufaux M. M. Lecadet 1989 Transformation and expression of a cloned δ-endotoxin gene in Bacillus thuringiensis FEMS Microbiology Letters 60 211 218
21. A. Pospiech B. Neumann 1995 A versatile quick-prep of genomic DNA form gram-positive bacteria Trends in Genetics 11 217 218
22. F. Sanger S. Nicklen A. R. Coulson 1977 DNA sequencing with chain-terminating inhibitors Proceedings of the National Academy of Sciences of the United States of America 74 5463 5467
23. J. E. Barboza-Corona J.C. Contreras R. Velázquez-Robledo M. Bautista-Justo R. Cruz-Camarillo J. E. Ibarra 1999 Selection of chitinolytic strain of Bacillus thuringiensis Biotechnology Letters 21 1125 1129
24. U. K. Laemli 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 680 685
25. A Ruiz-Sanchez R. Cruz-Camarillo R. Salcedo-Hernández J. Ibarra J. E. Barboza-Corona 2005 Molecular cloning and purification of an endochitinase from Serratia marcescens (Nima) Molecular Biotechnology 31 103 112
26. M. Hardt R. A. Laine 2004 Mutation of active site residues in the chitin-binding domain ChBDChiA1 from chitinase A1 of Bacillus circulans alters substrate specificity: Use of a green fluorescent protein binding assay Archives of Biochemistry and Biophysics 426 256 297
27. M. C. Chang P. L. Lai M. L. Wu 2004 Biochemical characterization and site-directed mutational analysis of the double chitin-binding domain from chitinase 92 of Aeromononas hydrophila JP101 FEMS Microbiology Letters 232 61 66
28. S. Ferrandon T. Sterzenbach F. B. Mersha M. Q. Xu 2003 A single surface tryptophan in the chitin-binding domain from Bacillus circulans chitinase A1 plays a pivotal role in binding chitin and can be modified to create an elutable affinity tag Biochimica et Biophysica Acta 1621 31 40
29. T. Watanabe K. Susuki W. Oyanagi K. Ohnishi H. Tanaka 1990 Gene cloning of chitinase A1 from Bacillus circulans WL-12 revealed its evolutionary relationship to Serratia chitinase and to the type III homology units of fibronectin Journal of Biological Chemistry 265 15659 15665
30. T. Watanabe W. Oyanagi K. Suzuki K. Ohnishi H. Tanaka 1992 Structure of the gene encoding chitinase D of Bacillus circulans WL-12 and possible homology of the enzyme to other prokaryotic chitinases and class III plant chitinases Journal of Bacteriology 174 408 414
31. L. Chernin L. de la Fuente V. Sovoleb S. Haran C. E. Vorgias A. B. Oppehneim I. Chet 1997 Molecualr cloning, structural análisis, and expresión in Escherichia coli of a chitinase gene from Enterobacter agglomerans Applied and Environmental Microbiology 63 834 839
32. C. Du K. W. Nickerson 1996 Bacillus thuringiensis HD-73 spores have surface-localized Cry1Ac toxin: Physiological and pathogenic consequences Applied and Environmental Microbiology 62 3722 3726
33. M. B. Brurberg V. G. H. Eijsink I. F. Nes 1994 Characterization of a chitinase gene (chiA) from Serratia marcescens BJL200 and one-step purification of the gene product FEMS Microbiology Letters 124 399 404
34. Lewin, B. (2000). Genes VII (pp. 147-149). New York: Oxford University Press Inc.