Evaluation of antidiabetic potentials of Phyllanthus niruri in alloxan diabetic rats

African Journal of Biotechnology

Volumn 9, Issue 2, 2010, Pages 248-259

  Okoli, C O ^a   Ibiam, A F ^a   Ezike, A C ^a   Akah, P A ^a   Okoye, T C ^a  

^a UNIVERSITY OF NIGERIA   (Nigeria)

Author keywords

Alloxan; Antidiabetic; Blood cells; Blood glucose; Pancreas; Phyllanthus niruri; Toxicity

Indexed keywords

ANTIDIABETIC AGENT; CHOLESTEROL; GLIBENCLAMIDE; GLUCOSE; HEMOGLOBIN; METHANOL EXTRACTION RESIDUE; PHYLLANTHUS NIRURI EXTRACT; POLYSORBATE 80; TRIACYLGLYCEROL;

ALLOXAN DIABETES MELLITUS; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BLOOD LEVEL; BODY WEIGHT; CELL DEATH; CHOLESTEROL BLOOD LEVEL; CONTROL GROUP; CONTROLLED STUDY; CYTOARCHITECTURE; DIET RESTRICTION; ERYTHROCYTE COUNT; FEMALE; GLUCOSE BLOOD LEVEL; HISTOLOGY; LD 50; LETHALITY; LEUKOCYTE COUNT; MALE; MEAL; MEDICINAL PLANT; NONHUMAN; PANCREAS; PHYLLANTHUS NIRURI; RAT; TISSUE NECROSIS; TRIACYLGLYCEROL BLOOD LEVEL;

EUPHORBIACEAE; MUS; PHYLLANTHUS NIRURI; RATTUS;

EID: 75749158540     PISSN: None     EISSN: 16845315     Source Type: Journal    
DOI: None     Document Type: Article

References (80)

1. Agrawal RP, Sharma P, Pal M, Kochar A, Kochar DK (2006). Magnitude of dyslipedemia and its association with micro and macro vascular complications in type 2 diabetes: A hospital based study from Bikaner (Northwest India). Diabetes Res. Clin. Pract. 73: 211-214
2. Ahmed I, Lakhani MS, Gillett M, John A, Raza H (2001). Hypotriglyceridemic and hypocholesterolemic effects of anti-diabetic Momordica charantia (karela) fruit extract in streptozotocin-induced diabetic rats. Diabetes Res. Clin. Pract. 51: 155-161.
3. Akah PA, Okoli CO, Nwafor SV (2002). Phytotherapy in the management of diabetes mellitus. J. Nat. Rem. 2: 1-10.
4. Aruna RV, Ramesh B, Kartha VN (1999). Effect of beta carotene on protein glycosylation in alloxan induced diabetic rats, Indian J. Exp. Biol. 32: 399-401.
5. Bagalkotkar G, Sagineedu SR, Saad MS, Stanslas J (2006). Phytochemicals from Phyllanthus niruri Linn. and their pharmacological properties: a review. J. Pharm. Pharmacol. 58: 1559-1570.
6. Balkau B (2000). The DECODE study, Diabetes epidemiology: collaborative analysis of diagnostic criteria in Europe. Diabetes Metab. 26: 282-286.
7. Barros ME, Lima R, Mercuri LP, Matos JR, Schor N, Boim MA (2006). Effect of extract of Phyllanthus niruri on crystal deposition in experimental urolithiasis. Urol. Res. 34(6): 351-357.
8. Barros ME, Schor N, Boim MA (2003). Effects of an aqueous extract from Phyllantus niruri on calcium oxalate crystallization in vitro. Urol. Res. 30(6): 374-9.
9. Bavarva JH, Narasimhacharya AVRL (2008). Antihyperglycemic and Hypolipidemic Effects of Costus speciosus in Alloxan induced Diabetic Rats. Phytother. Res. 22: 620-626.
10. Bhat R, Bhansali A, Bhadada S, Sialy R (2007). Effect of pioglitazone therapy in lean type1 diabetes mellitus. Diabetes Res. Clin. Pract. 78: 349-354.
11. Bhattacharjee R, Sil PC (2006). The Protein Fraction of Phyllanthus niruri Plays a Protective Role against Acetaminophen Induced Hepatic Disorder via its Antioxidant Properties. Phytother. Res. 20: 595-601.
12. Bhattacharjee R, Sil PC (2007). Protein isolate from the herb, Phyllanthus niruri L. (Euphorbiaceae), plays hepatoprotective role against carbon tetrachloride induced liver damage via its antioxidant properties. Food Chem. Toxicol. 45(5): 817-26.
13. Brown GB, Xue-Qiao Z, Sacco DE, Alberts JJ (1993). Lipid lowering and plaque regression. New insights into prevention of plaque disruption and clinical events in coronary disease. Circulation, 87: 1781-1791.
14. Campos AH, Schor N (1999). Phyllanthus niruri inhibits calcium oxalate endocytosis by renal tubular cells: its role in urolithiasis. Nephron. 81(4): 393-397.
15. Ceriello A (2005). Postprandial hyperglycemia and diabetes complications; is it time to treat? Diabetes, 54: 1-7.
16. Chatterjee M, Sarkar K, Sil PC (2006). Herbal (Phyllanthus niruri) protein isolate protects liver from nimesulide induced oxidative stress. Pathophysiology, 13(2): 95-102.
17. Chatterjee M, Sil PC (2006). Hepatoprotective effect of aqueous extract of Phyllanthus niruri on nimesulide-induced oxidative stress in vivo. Indian J. Biochem. Biophys. 43(5): 299-305.
18. Choi JS, Yokozawa T, Oura H (1991). Improvement of hyperglycemia and hyperlipemia in streptozotocin-diabetic rats by a methanolic extract of Prunus daidiana stems and its main component, prunin, Planta Med. 57: 208-211.
19. Chopra RN, Nayar SL, Chopra IC (1986). Glosssary of Indian Medicinal Plants, CSIR, New Delhi Catholic Press, Ranchi, India. Cimanga RK, Tona L, Luyindula N, Mesia K, Lusakibanza M, Musuamba CT, Apers S, De Bruyne T, Van Miert S, Hermans N, Totté J, Pieters L, Vlietinck AJ (2004). In vitro antiplasmodial activity of callus culture extracts and fractions from fresh apical stems of Phyllanthus niruri L. (Euphorbiaceae): part 2. J. Ethnopharmacol. 95(2-3): 399-404.
20. Colca JR (2006). Insulin sensitizers may prevent metabolic inflammation. Biochem. Pharmacol. 72: 125-131.
21. De Sousa E, Zanatta L, Seifriz I, Creczynski-Pasa TB, Pizzolatti MG, Szpoganicz B, Silva FRMB (2004). Hypoglycemic effect and antioxidant potential of kaempferol-3, 7-O-(-)-dirhamnoside from Bauhinia forficate leaves. J. Nat. Prod. 67: 829-832.
22. Elfahmi, Batterman S, Koulman A, Hackl T, Bos R, Kayser O, Woerdenbag HJ, Quax WJ (2006) Lignans from cell suspension cultures of Phyllanthus niruri, an Indonesian medicinal plant. J. Nat. Prod. 69(1): 55-58.
23. Freitas AM, Schor N, Boim MA (2002). The effect of Phyllanthus niruri on urinary inhibitors of calcium oxalate crystallization and other factors associated with renal stone formation. BJU Int. 89(9): 829-834.
24. Fröde TS, Medeiros YS (2008). Animal models to test drugs with potential antidiabetic activity. J. Ethnopharmacol. 115: 173-183.
25. Girach RD, Siddioui PA, Khan SA (1994). Traditional plant remedies among the kondh (Orissa), Int. J. Pharmacol. 32: 274-283.
26. nd ed., Chapman and Hall, London, p. 282.
27. Hayden MR, Tyagi SC, Kerklo MM, Nicolls MR (2005). Type 2 Diabetes Mellitus as a Conformational Disease. JOP. J. Pancreas (Online), 6(4): 287-302
28. Iizuka T, Moriyama H, Nagai M (2006). Vasorelaxant effects of methyl brevifolincarboxylate from the leaves of Phyllanthus niruri. Biol. Pharm. Bull. 29(1): 177-179.
29. Iizuka T, Nagai M, Taniguchi A, Moriyama H, Hoshi K (2007). Inhibitory effects of methyl brevifolincarboxylate isolated from Phyllanthus niruri L. on platelet aggregation. Biol. Pharm. Bull. 30(2): 382-384.
30. Jagetia GC, Baliga MS (2004). The evaluation of nitric oxide scavenging activity of certain Indian medicinal plants in vitro: a preliminary study. J. Med. Food, 7(3): 343-348.
31. Khanna AK, Rizvi F, Chander R (2002). Lipid lowering activity of Phyllanthus niruri in hyperlipemic rats. J. Ethnopharmacol. 82: 19-22.
32. Kieley S, Dwivedi R, Monga M (2008). Ayurvedic medicine and renal calculi. J. Endourol. 22(8): 1613-1616.
33. Lee JH, Park JW, Kim JS, Park BH, Rho HW (2008). Protective Effect of Amomi Semen Extract on Alloxan-induced Pancreatic β-Cell Damage. Phytother. Res. 22: 86-90.
34. Lenzen S, Tiedge M, Jorns A, Munday R (1996). Alloxan derivatives as a tool for the elucidation of the mechanism of the diabetogenic action of alloxan. In: Shafrir, E. (ed.) Lessons from Animal Diabetes, Birkhauser, Boston, pp. 113-122.
35. Leung KK, Leung PS (2008). Effects of Hyperglycemia on the Angiotensin II Receptor Type 1 Expression and Insulin Secretion in an INS-1E Pancreatic Beta-Cell Line. JOP. J. Pancreas (Online) 9(3): 290-299.
36. Lin TJ, Su CC, Lan CK, Jiang DD, Tsai JL, Tsai MS (2003). Acute poisonings with Breynia officinalis-an outbreak of hepatotoxicity. J. Toxicol. Clin. Toxicol. 41: 591-594.
37. Lorke D (1983). A new approach to practical acute toxicity testing. Arch. Toxicol. 53: 275-289.
38. Manjrekar AP, Jisha V, Bag PP, Adhikary B, Pai MM, Hegde A, Nandini M (2008). Effect of Phyllanthus niruri Linn. treatment on liver, kidney and testes in CCl4 induced hepatotoxic rats. Indian J. Exp. Biol. 46(7): 514-520.
39. Mellinger CG, Carbonero ER, Noleto GR, Cipriani TR, Oliveira MB, Gorin PA, Iacomini M (2005). Chemical and biological properties of an arabinogalactan from Phyllanthus niruri. J. Nat. Prod. 68(10): 1479-1483.
40. Mellinger CG, Cipriani TR, Noleto GR, Carbonero ER, Oliveira MB, Gorin PA, Iacomini M (2008). Chemical and immunological modifications of an arabinogalactan present in tea preparations of Phyllanthus niruri after treatment with gastric fluid. Int. J. Biol. Macromol. 43(2): 115-120.
41. Micali S, Sighinolfi MC, Celia A, De Stefani S, Grande M, Cicero AF, Bianchi G (2006). Can Phyllanthus niruri affect the efficacy of extracorporeal shock wave lithotripsy for renal stones? A randomized, prospective, long-term study. J. Urol. 176(3): 1020-1022.
42. Mohan V, Farooq S, Deepa M (2008). Prevalence of Fibrocalculous Pancreatic Diabetes in Chennai in South India. J. Pancreas (Online) 9(4): 489-492.
43. Monnier L, Lapinski H, Colette C (2003). Contributions of fasting and postprandial plasma glucose increments to the overall diurnal hyperglycemia of type2 diabetes patients. Diabetes Care, 26: 881-885.
44. Mulchandani NB, Hassarajani SA (1984). 4-Methoxy-nor-Securinine, a New Alkaloid from Phyllanthus niruri. Planta Med. 50(1): 104-105.
45. Muller PH, Schmulling RM, Eggstein M (1977). A fully enzymatic triglyceride determination. J. Clin. Chem. 15: 457-504.
46. Murugaiyah V, Chan KL (2006). Antihyperuricemic lignans from the leaves of Phyllanthus niruri. Planta Med. 72(14): 1262-1267.
47. Murugaiyah V, Chan KL (2007). Determination of four lignans in Phyllanthus niruri L. by a simple high-performance liquid chromatography method with fluorescence detection. J. Chromatogr. A. 1154(1-2): 198-204.
48. Mustofa, Sholikhah EN, Wahyuono S (2007). In vitro and in vivo antiplasmodial activity and cytotoxicity of extracts of Phyllanthus niruri L. herbs traditionally used to treat malaria in Indonesia. Southeast Asian J. Trop. Med. Public Health, 38(4): 609-615.
49. Naik AD, Juvekar AR (2003). Effects of alkaloidal extract of Phyllanthus niruri on HIV replication. Indian J. Med. Sci. 57(9): 387-393.
50. Nishiura JL, Campos AH, Boim MA, Heilberg IP, Schor N (2004). Phyllanthus niruri normalizes elevated urinary calcium levels in calcium stone forming (CSF) patients. Urol. Res. 32(5): 362-6.
51. Ogata T, Higuchi H, Mochida S, Matsumoto H, Kato A, Endo T, Kaji A, Kaji H (1992). HIV-1 reverse transcriptase inhibitor from Phyllanthus niruri. AIDS Res. Hum. Retroviruses, 8(11): 1937-1944.
52. Olive-Bever B (1986). Medicinal plants in Tropical West Africa. Cambridge University Press, Cambridge.
53. Paranjape P (2001). Indian Medicinal Plants: Forgotten Healers. Chaukhamba Sanskrit Pratisthan, Delhi, p. 48.
54. Platel K, Shurpalekar KS, Srinivasan K (1993). Effect of bitter gourd (Momordica charantia) on growth and blood constituents in albino rats. Nahrung, 37: 156-160.
55. Prince PSM, Menon VP, Gunasekaran G (1999). Hypolipidaemic action of Tinospora cordifolia roots in alloxan diabetic rats. J. Ethnopharmacol. 64: 53-57.
56. Qian-Cutrone J, Huang S, Trimble J, Li H, Lin PF, Alam M, Klohr SE, Kadow KF (1996). Niruriside, a new HIV REV/RRE binding inhibitor from Phyllanthus niruri. J. Nat. Prod. 59(2): 196-199.
57. Ramakrishnan PN, Murugesan R, Palanichamy S (1982). Oral hypoglycaemic effect of Phyllanthus niruri leaves. Indian J. Pharm. Sci. 44: 10-12
58. Rao BK, Kesavulu MM, Giri R, Rao CA (1999). Antidiabetic and hypolipidemic effects of Momordica cymbalaria Hook. fruit powder in alloxan-diabetic rats J. Ethnopharmacol. 67: 103-109.
59. Santos AR, Filho VC, Niero R, Viana AM, Moreno FN, Campos MM, Yunes RA, Calixto JB (1994). Analgesic effects of callus culture extracts from selected species of Phyllanthus in mice. J Pharm. Pharmacol. 46(9): 755-759.
60. Santos AR, Filho VC, Yunes RA, Calixto JB (1995). Analysis of the mechanisms underlying the antinociceptive effect of the extracts of plants from the genus Phyllanthus. Gen. Pharmacol. 26(7): 1499-1506.
61. Sarkar MK, Sil PC (2007). Hepatocytes are protected by herb Phyllanthus niruri protein isolate against thioacetamide toxicity. Pathophysiology, 14(2): 113-120.
62. Shakil NA, Pankaj, Kumar J, Pandey RK, Saxena DB (2008). Nematicidal prenylated flavanones from Phyllanthus niruri. Phytochemistry, 69(3): 759-764.
63. Sharma SR, Dwivedi SK, Swarup D (1997). Hypoglycaemic, antihyperglycaemic and hypolipidemic activities of Caesalpinia bonducella seeds in rats. J. Ethnopharmacol. 58: 39-44.
64. Shimizu M, Horie S, Terashima S, Ueno H, Hayashi T, Arisawa M, Suzuki S, Yoshizaki M, Morita N (1989). Studies on aldose reductase inhibitors from natural products. II. Active components of a Paraguayan crude drug Para-parai mí, Phyllanthus niruri. Chem. Pharm. Bull. (Tokyo). 37(9): 2531-2532.
65. Subeki S, Matsuura H, Takahashi K, Yamasaki M, Yamato O, Maede Y, Katakura K, Kobayashi S, Trimurningsih T, Chairul C, Yoshihara T (2005). Anti-babesial and anti-plasmodial compounds from Phyllanthus niruri. J. Nat. Prod. 68(4): 537-539.
66. Syamasundar KV, Singh B, Thakur RS, Husain A, Kiso Y, Hikino H (1985). Antihepatotoxic principles of Phyllanthus niruri herb. J. Ethnopharmacol. 14: 41-44.
67. Szudelski T (2001). The mechanism of alloxan and streptozotocin action in β cells of the rat pancreas. Physiol. Res. 50: 536-546.
68. Tanaka S, Kobayashi T, Nakanishi K, Okubo M, Murase T, Hashimoto M (2001). Evidence of primary beta-cell destruction by T-cells and beta-cell differentiation from pancreatic ductal cells in diabetes associated with active autoimmune chronic pancreatitis. Diabetes Care, 24: 1661-1667.
69. Tanaka S, Kobayashi T, Nakanishi K, Okubo M, Murase T, Hashimoto M, et al. (2000). Corticosteroid-responsive diabetes mellitus associated with autoimmune pancreatitis. Lancet, 356: 910-911.
70. Tasaduq SA, Singh K, Sethi S, Sharma SC, Bedi KL, Singh J, Jaggi BS, Johri RK (2003). Hepatocurative and antioxidant profile of HP-1, a polyherbal phytomedicine. Hum. Exp. Toxicol. 22(12): 639-645.
71. The Diabetes Control and Complications Trial Research Group (1993). The effect of intensive treatment of diabetes on the development and progression of long-term complications in insulin-dependent diabetes mellitus, N. Engl. J. Med. 329: 977-986.
72. Tona L, Cimanga RK, Mesia K, Musuamba CT, De Bruyne T, Apers TS, Hernans N, Van Miert S, Pieters L, Totté J, Vlietinck AJ (2004). In vitro antiplasmodial activity of extracts and fractions from seven medicinal plants used in the Democratic Republic of Congo. J. Ethnopharmacol. 93: 27-32
73. Tona L, Mesia, K, Ngimbi NP, Chrimwami B, Okond'Ahoka A, Cimanga K, De Bruyne T, Apers S, Hermans N, Totté J, Pieters L, Vlietinck AJ (2001). In vivo antimalarial activity of Cassia occidentalis, Morinda morindoides and Phyllanthus nfruri. Ann. Trop. Med. Parasitol. 65: 47-57.
74. Tona L, Ngimbi NP, Tsakala M, Mesia K, Cimanga K, Apers S, De Bruyne T, Pieters L, Tott́e J, Vlietinck AJ (1999). Antimalarial activity of 20 crude extracts from nine African medicinal plants used in Kinshasa, Congo. J. Ethnopharmacol. 68: 193-203.
75. Trease GE, Evans WC (1983). Drugs of Biological origin. In: Pharmacognosy,12th ed., Balliere Tindall, United Kingdom, pp. 309-540.
76. Unander DW, Webster GL, Blumberg BS (1995). Usage and bioassays in Phyllanthus (Euphorbiaceae). IV. Clustering of antiviral uses and other effects. J. Ethnopharmacol. 45: 1-18.
77. Verspohl EJ (2002). Recommended testing in diabetes research. Planta Medica, 68: 581-590.
78. Wang JJ, Qiao Q, Miettinen ME, Lappalainen J, Hu G, Tuomilehto J (2004). The metabolic syndrome defined by factor analysis and incident type 2 diabetes in a Chinese population with high postprandial glucose. Diabetes Care, 27: 2429-2437
79. Yoshida K, Toki F, Takeuchi T, Watanabe S, Shiratori K, Hayashi N (1995). Chronic pancreatitis caused by an autoimmune abnormality. Proposal of the concept of autoimmune pancreatitis. Dig. Dis. Sci. 40: 1561-1568.
80. Zanatta L, De Sousa E, Cazarolli LH, Cunha Jr. A, Pizzolatti AMG, Szpoganicz B, Silva FRMB (2007). Effect of crude extract and fractions from Vitex megapotamica leaves on hyperglycemia in alloxan-diabetic rats. J. Ethnopharmacol. 109: 151-155.