Simple approach to reduce PCR artefact formation leads to reliable genotyping of MHC and other highly polymorphic loci - Implications for evolutionary analysis

Gene

Volumn 427, Issue 1-2, 2008, Pages 117-123

  Lenz, Tobias L ^a   Becker, Sven ^a  

^a MAX PLANCK INSTITUTE FOR EVOLUTIONARY BIOLOGY   (Germany)

Author keywords

Gasterosteus aculeatus; Major Histocompatibility Complex; Multi template PCR; PCR artifacts; Three spined stickleback

Indexed keywords

MAJOR HISTOCOMPATIBILITY ANTIGEN; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 2;

ARTICLE; ARTIFACT; CONTROLLED STUDY; DNA POLYMORPHISM; FISH; GASTEROSTEUS ACULEATUS; GENE AMPLIFICATION; GENE SEQUENCE; GENETIC ANALYSIS; GENOTYPE; MOLECULAR EVOLUTION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ALLELES; ARTIFACTS; BASE SEQUENCE; CLONING, MOLECULAR; GENETIC TECHNIQUES; GENOTYPE; HUMANS; MAJOR HISTOCOMPATIBILITY COMPLEX; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; POLYMORPHISM, GENETIC; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, NUCLEIC ACID;

GASTEROSTEUS ACULEATUS; VERTEBRATA;

EID: 55149119144     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.gene.2008.09.013     Document Type: Article

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