Dual affinity method for plasmid DNA purification in aqueous two-phase systems

Journal of Chromatography A

Volumn 1217, Issue 9, 2010, Pages 1429-1436

  Barbosa, H S C ^a   Hine, A V ^b   Brocchini, S ^c   Slater, N K H ^d   Marcos, J C ^a  

^a UNIVERSITY OF MINHO   (Portugal)

^b ASTON UNIVERSITY   (United Kingdom)

^c UNIVERSITY OF LONDON   (United Kingdom)

^d UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Affinity ligand; Aqueous two phase systems; Bacterial cell lysate; Immobilized metal ion affinity partitioning; Lac repressor; Plasmid purification

Indexed keywords

AFFINITY LIGANDS; AFFINITY PARTITIONING; AQUEOUS TWO PHASE SYSTEM; BACTERIAL CELL LYSATE; IMMOBILIZED METAL-ION AFFINITY PARTITIONING; IMMOBILIZED METALS; LAC REPRESSOR; PLASMID PURIFICATION;

BACTERIOLOGY; DEXTRAN; DNA; ELECTROPHORESIS; ETHYLENE; ETHYLENE GLYCOL; GENES; GLUCOSE; LIGANDS; METAL RECOVERY; METAL REFINING; POLYETHYLENE GLYCOLS; POLYETHYLENE OXIDES; PROTEINS; PURIFICATION; RNA; SMELTING;

CHEMICALS REMOVAL (WATER TREATMENT);

BUFFER; DEXTRAN; DNA BINDING PROTEIN; GENOMIC DNA; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MACROGOL; PHOSPHATE; PLASMID DNA; RNA;

AGAR GEL ELECTROPHORESIS; AQUEOUS SOLUTION; ARTICLE; CELL LYSATE; CONTROLLED STUDY; DNA EXTRACTION; DNA PURIFICATION; ELUTION; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL;

CHROMATOGRAPHY, AFFINITY; DNA; ELECTROPHORESIS, AGAR GEL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GREEN FLUORESCENT PROTEINS; HISTIDINE; IMINO ACIDS; LAC REPRESSORS; LACTOGLOBULINS; OLIGOPEPTIDES; PLASMIDS; POLYETHYLENE GLYCOLS; RECOMBINANT FUSION PROTEINS;

BACTERIA (MICROORGANISMS);

EID: 75349103002     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2009.12.059     Document Type: Article

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