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Volumn 852, Issue 1-2, 2007, Pages 409-419
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Effect of surface histidine mutations and their number on the partitioning and refolding of recombinant human granulocyte-colony stimulating factor (Cys17Ser) in aqueous two-phase systems containing chelated metal ions
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Author keywords
Aqueous two phase systems; Granulocyte colony stimulating factor; Inclusion bodies; Metal affinity partitioning; Protein refolding
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Indexed keywords
CHELATION;
ESCHERICHIA COLI;
METAL IONS;
PH EFFECTS;
PROTEINS;
PURIFICATION;
AQUEOUS TWO-PHASE SYSTEMS;
GRANULOCYTE-COLONY STIMULATING FACTOR;
INCLUSION BODIES;
METAL AFFINITY PARTITIONING;
PROTEIN REFOLDING;
GENETIC ENGINEERING;
DEXTRAN;
HISTIDINE;
IMIDAZOLE;
MACROGOL;
METAL ION;
MUTANT PROTEIN;
RECOMBINANT GRANULOCYTE COLONY STIMULATING FACTOR;
ANIMAL CELL;
ARTICLE;
BINDING AFFINITY;
CELL INCLUSION;
CHELATION;
ESCHERICHIA COLI;
GRANULOCYTE;
METAL BINDING;
MOUSE;
NONHUMAN;
PRIORITY JOURNAL;
PROTEIN AGGREGATION;
PROTEIN BINDING;
PROTEIN EXPRESSION;
PROTEIN FOLDING;
PROTEIN INTERACTION;
PROTEIN PURIFICATION;
ANIMALS;
CELL LINE, TUMOR;
CHELATING AGENTS;
CYSTEINE;
ELECTROPHORESIS, POLYACRYLAMIDE GEL;
GRANULOCYTE COLONY STIMULATING FACTOR, RECOMBINANT;
HISTIDINE;
METALS;
MICE;
MUTATION;
PROTEIN FOLDING;
SERINE;
WATER;
ESCHERICHIA COLI;
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EID: 34249722205
PISSN: 15700232
EISSN: None
Source Type: Journal
DOI: 10.1016/j.jchromb.2007.01.051 Document Type: Article |
Times cited : (7)
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References (40)
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