Preparation of plasmid DNA polyplexes from alkaline lysates by a two-step aqueous two-phase extraction process

Journal of Chromatography A

Volumn 1164, Issue 1-2, 2007, Pages 105-112

  Duarte, Sónia P ^a   Fortes, António G ^a   Prazeres, Duarte M F ^b   Marcos, João C ^a  

^a UNIVERSITY OF MINHO   (Portugal)

^b INSTITUTO SUPERIOR TÉCNICO   (Portugal)

Author keywords

Aqueous two phase systems; Gene therapy; Liquid liquid extraction; Plasmid DNA; Polyethyleneimine; Polyplexes; Purification

Indexed keywords

AQUEOUS TWO-PHASE SYSTEMS; GENE THERAPY; LIQUID LIQUID EXTRACTION; PLASMID DNA; POLYETHYLENEIMINE; POLYPLEXES;

DNA; LIGANDS; POLYETHYLENE GLYCOLS; PURIFICATION;

POLYMERS;

AMMONIUM SULFATE; DEXTRAN; MACROGOL; PLASMID DNA; POLYETHYLENEIMINE; POLYMER; RNA; SODIUM CHLORIDE;

ALKALINITY; AQUEOUS SOLUTION; ARTICLE; DNA EXTRACTION; DNA PURIFICATION; GENE TARGETING; LIQUID LIQUID EXTRACTION; METHODOLOGY; MOLECULAR INTERACTION; PARTICLE SIZE; PHASE SEPARATION; PRIORITY JOURNAL;

CHEMICAL FRACTIONATION; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, AGAR GEL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; PLASMIDS; POLYETHYLENE GLYCOLS; POLYETHYLENEIMINE; REPRODUCIBILITY OF RESULTS;

EID: 34548030710     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2007.06.061     Document Type: Article

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