Purification and characterization of mammalian glucose transporters expressed in Pichia pastoris

Protein Expression and Purification

Volumn 70, Issue 1, 2010, Pages 81-87

  Alisio, Arturo ^a   Mueckler, Mike ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Glucose transporter; GLUT1; GLUT4; Membrane protein purification

Indexed keywords

GLUCOSE TRANSPORTER 1; GLUCOSE TRANSPORTER 2; SLC2A2 PROTEIN, RAT;

ANIMAL; ARTICLE; CHEMISTRY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; PICHIA; RAT; X RAY DIFFRACTION;

ANIMALS; GLUCOSE TRANSPORTER TYPE 1; GLUCOSE TRANSPORTER TYPE 2; HUMANS; PICHIA; RATS; X-RAY DIFFRACTION;

MAMMALIA; PICHIA PASTORIS;

EID: 75349092314     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.10.011     Document Type: Article

References (49)

1. Pao S.S., Paulsen I.T., and Saier Jr. M.H. Major facilitator superfamily. Microbiol. Mol. Biol. Rev. 62 (1998) 1-34
2. Nie Y., Smirnova I., Kasho V., and Kaback H.R. Energetics of ligand-induced conformational flexibility in the lactose permease of Escherichia coli. J. Biol. Chem. 281 (2006) 35779-35784
3. Abramson J., Iwata S., and Kaback H.R. Lactose permease as a paradigm for membrane transport proteins. Mol. Membr. Biol. 21 (2004) 227-236 (Review)
4. Abramson J., Smirnova I., Kasho V., Verner G., Kaback H.R., and Iwata S. Structure and mechanism of the lactose permease of Escherichia coli. Science 301 (2003) 610-615
5. Guan L., Mirza O., Verner G., Iwata S., and Kaback H.R. Structural determination of wild-type lactose permease. Proc. Natl. Acad. Sci. USA 104 (2007) 15294-15298
6. Huang Y., Lemieux M.J., Song J., Auer M., and Wang D.N. Structure and mechanism of the glycerol-3-phosphate transporter from Escherichia coli. Science 301 (2003) 616-620
7. Yin Y., He X., Szewczyk P., Nguyen T., and Chang G. Structure of the multidrug transporter EmrD from Escherichia coli. Science 312 (2006) 741-744
8. Uldry M., and Thorens B. The SLC2 family of facilitated hexose and polyol transporters. Pflugers Arch. 447 (2004) 480-489
9. Kahn B.B. Lilly lecture 1995. Glucose transport: pivotal step in insulin action. Diabetes 45 (1996) 1644-1654
10. Ganapathy V., Thangaraju M., and Prasad P.D. Nutrient transporters in cancer: relevance to Warburg hypothesis and beyond. Pharmacol. Ther. 121 (2009) 29-40
11. De Vivo D.C., Wang D., Pascual J.M., and Ho Y.Y. Glucose transporter protein syndromes. Int. Rev. Neurobiol. 51 (2002) 259-288
12. Santer R., Schneppenheim R., Dombrowski A., Gotze H., Steinmann B., and Schaub J. Fanconi-Bickel syndrome - a congenital defect of the liver-type facilitative glucose transporter. SSIEM Award. Society for the Study of Inborn Errors of Metabolism. J. Inherit. Metab. Dis. 21 (1998) 191-194
13. Hruz P.W., Murata H., Qiu H., and Mueckler M. Indinavir induces acute and reversible peripheral insulin resistance in rats. Diabetes 51 (2002) 937-942
14. Graybill C., van Hoek A.N., Desai D., Carruthers A.M., and Carruthers A. Ultrastructure of human erythrocyte GLUT1. Biochemistry 45 (2006) 8096-8107
15. Hruz P.W., and Mueckler M.M. Structural analysis of the GLUT1 facilitative glucose transporter. Mol. Membr. Biol. 18 (2001) 183-193 (Review)
16. Alisio A., and Mueckler M. Relative proximity and orientation of helices 4 and 8 of the GLUT1 glucose transporter. J. Biol. Chem. 279 (2004) 26540-26545
17. Mueckler M., and Makepeace C. Transmembrane segment 12 of the Glut1 glucose transporter is an outer helix and is not directly involved in the transport mechanism. J. Biol. Chem. 281 (2006) 11550-11555
18. Mueckler M., and Makepeace C. Model of the exofacial substrate-binding site and helical folding of the human Glut1 glucose transporter based on scanning mutagenesis. Biochemistry 48 (2009) 5934-5942
19. Blodgett D.M., Graybill C., and Carruthers A. Analysis of glucose transporter topology and structural dynamics. J. Biol. Chem. 283 (2008) 36416-36424
20. Blodgett D.M., and Carruthers A. Quench-flow analysis reveals multiple phases of GluT1-mediated sugar transport. Biochemistry 44 (2005) 2650-2660
21. Baldwin S.A., Baldwin J.M., and Lienhard G.E. Monosaccharide transporter of the human erythrocyte. Characterization of an improved preparation. Biochemistry 21 (1982) 3836-3842
22. Boulter J.M., and Wang D.N. Purification and characterization of human erythrocyte glucose transporter in decylmaltoside detergent solution. Protein Expr. Purif. 22 (2001) 337-348
23. Kasahara M., and Hinkle P.C. Reconstitution and purification of the d-glucose transporter from human erythrocytes. J. Biol. Chem. 252 (1977) 7384-7390
24. Graham T.E., and Kahn B.B. Tissue-specific alterations of glucose transport and molecular mechanisms of intertissue communication in obesity and type 2 diabetes. Horm. Metab. Res. 39 (2007) 717-721
25. Murata H., Hruz P.W., and Mueckler M. The mechanism of insulin resistance caused by HIV protease inhibitor therapy. J. Biol. Chem. 275 (2000) 20251-20254
26. Mueckler M., Caruso C., Baldwin S.A., Panico M., Blench I., Morris H.R., Allard W.J., Lienhard G.E., and Lodish H.F. Sequence and structure of a human glucose transporter. Science 229 (1985) 941-945
27. James D.E., Strube M., and Mueckler M. Molecular cloning and characterization of an insulin-regulatable glucose transporter. Nature 338 (1989) 83-87
28. Mueckler M., Weng W., and Kruse M. Glutamine 161 of Glut1 glucose transporter is critical for transport activity and exofacial ligand binding. J. Biol. Chem. 269 (1994) 20533-20538
29. Marshall B.A., Murata H., Hresko R.C., and Mueckler M. Domains that confer intracellular sequestration of the Glut4 glucose transporter in Xenopus oocytes. J. Biol. Chem. 268 (1993) 26193-26199
30. Holman G.D. Side-specific photolabelling of the hexose transporter. Biochem. Soc. Trans. 17 (1989) 438-440
31. Enkvetchakul D., Jeliazkova I., Bhattacharyya J., and Nichols C.G. Control of inward rectifier K channel activity by lipid tethering of cytoplasmic domains. J. Gen. Physiol. 130 (2007) 329-334
32. Long S.B., Tao X., Campbell E.B., and MacKinnon R. Atomic structure of a voltage-dependent K+ channel in a lipid membrane-like environment. Nature 450 (2007) 376-382
33. Zoccoli M.A., Baldwin S.A., and Lienhard G.E. The monosaccharide transport system of the human erythrocyte. Solubilization and characterization on the basis of cytochalasin B binding. J. Biol. Chem. 253 (1978) 6923-6930
34. Kayano T., Burant C.F., Fukumoto H., Gould G.W., Fan Y.S., Eddy R.L., Byers M.G., Shows T.B., Seino S., and Bell G.I. Human facilitative glucose transporters. Isolation, functional characterization, and gene localization of cDNAs encoding an isoform (GLUT5) expressed in small intestine, kidney, muscle, and adipose tissue and an unusual glucose transporter pseudogene-like sequence (GLUT6). J. Biol. Chem. 265 (1990) 13276-13282
35. Wellner M., Monden I., and Keller K. From triple cysteine mutants to the cysteine-less glucose transporter GLUT1: a functional analysis. FEBS Lett. 370 (1995) 19-22
36. Koumanov F., Yang J., Jones A.E., Hatanaka Y., and Holman G.D. Cell-surface biotinylation of GLUT4 using bis-mannose photolabels. Biochem. J. 330 Pt. 3 (1998) 1209-1215
37. Baldwin J.M., Lienhard G.E., and Baldwin S.A. The monosaccharide transport system of the human erythrocyte. Orientation upon reconstitution. Biochim. Biophys. Acta 599 (1980) 699-714
38. Widdas W.F. Facilitated transfer of hexoses across the human erythrocyte membrane. J. Physiol. 125 (1954) 163-180
39. Mueckler M. Facilitative glucose transporters. Eur. J. Biochem. 219 (1994) 713-725
40. Olson A.L., and Pessin J.E. Structure, function, and regulation of the mammalian facilitative glucose transporter gene family. Annu. Rev. Nutr. 16 (1996) 235-256
41. Zhao F.Q., and Keating A.F. Functional properties and genomics of glucose transporters. Curr. Genomics 8 (2007) 113-128
42. De Vivo D.C., Leary L., and Wang D. Glucose transporter 1 deficiency syndrome and other glycolytic defects. J. Child Neurol. 17 Suppl. 3 (2002) 3S15-3S23 (discussion 3S24-3S25)
43. Flier J.S., Mueckler M.M., Usher P., and Lodish H.F. Elevated levels of glucose transport and transporter messenger RNA are induced by ras or src oncogenes. Science 235 (1987) 1492-1495
44. Lemieux M.J. Eukaryotic major facilitator superfamily transporter modeling based on the prokaryotic GlpT crystal structure. Mol. Membr. Biol. 24 (2007) 333-341
45. Law C.J., Maloney P.C., and Wang D.N. Ins and outs of major facilitator superfamily antiporters. Annu. Rev. Microbiol. 62 (2008) 289-305
46. Ren Q., and Paulsen I.T. Comparative analyses of fundamental differences in membrane transport capabilities in prokaryotes and eukaryotes. PLoS Comput. Biol. 1 (2005) e27
47. Chang A.B., Lin R., Keith Studley W., Tran C.V., and Saier Jr. M.H. Phylogeny as a guide to structure and function of membrane transport proteins. Mol. Membr. Biol. 21 (2004) 171-181
48. Long S.B., Campbell E.B., and Mackinnon R. Crystal structure of a mammalian voltage-dependent Shaker family K+ channel. Science 309 (2005) 897-903
49. Jiang Y.X., Lee A., Chen J.Y., Ruta V., Cadene M., Chait B.T., and MacKinnon R. X-ray structure of a voltage-dependent K+ channel. Nature 423 (2003) 33-41