Structural analysis of the GLUT1 facilitative glucose transporter (Review)

Molecular Membrane Biology

Volumn 18, Issue 3, 2001, Pages 183-193

  Hruz, P W ^a   Mueckler, M M ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Membrane topology; Monosaccharide transport protein; Protein structure; Site directed mutagenesis; Structure activity relationships

Indexed keywords

AQUAPORIN; CARRIER PROTEIN; GLUCOSE TRANSPORTER; GLUCOSE TRANSPORTER 1; MONOSACCHARIDE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; CHEMICAL REACTION KINETICS; CRYSTAL STRUCTURE; GLUCOSE HOMEOSTASIS; GLUCOSE TRANSPORT SYSTEM; HUMAN; HUMAN CELL; MEMBRANE STRUCTURE; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; REVIEW; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

AFFINITY LABELS; AMINO ACID SEQUENCE; GLUCOSE; GLUCOSE TRANSPORTER TYPE 1; HUMANS; KINETICS; MOLECULAR SEQUENCE DATA; MONOSACCHARIDE TRANSPORT PROTEINS; MUTATION; PROTEIN CONFORMATION; SUBSTRATE SPECIFICITY;

EID: 0034798447     PISSN: 09687688     EISSN: None     Source Type: Journal    
DOI: 10.1080/09687680110072140     Document Type: Review

References (84)

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15. Exofacial photolabelling of the human erythrocyte glucose transporter with an azitrifluoroethyl-benzoyl-substituted bismannose
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31. Site-directed mutagenesis of GLUT1 in helix 7 residue 282 results in perturbation of exofacial ligand binding
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39. GLUTX1, a novel mammalian glucose transporter expressed in the central nervous system and insulin-sensitive tissues
40. Replacement of both tryptophan residues at 388 and 412 completely abolished cytochalasin B photolabelling of the GLUT1 glucose transporter
41. Radiation inactivation target size of rat adipocyte glucose transporters in the plasma membrane and intracellular pools
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52. Active and monomeric human red cell glucose transporter after high performance molecular-sieve chromatography in the presence of octyl glucoside and phosphatidylserine or phosphatidylcholine
53. Mammalian and bacterial sugar transport proteins are homologous
54. Transmembrane segment 5 of the Glut1 glucose transporter is an amphipathic helix that forms part of the sugar permeation pathway
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56. Molecular cloning of a novel member of the GLUT family of transporters, SLC2A10 (GLUT10), localized on chromosome 20q13.1: A candidate gene for NIDDM susceptibility
57. Substitution of tyrosine 293 of GLUT1 locks the transporter into an outward facing conformation
58. Identification of an amino acid residue that lies between the exofacial vesibule and exofacial substrate-binding site of the Glut1 sugar permeation pathway
59. Sequence and structure of a human glucose transporter
60. A mutation in the Glut2 glucose transporter gene of a diabetic patient abolishes transport activity
61. Glutamine 161 of Glut1 glucose transporter is critical for transport activity and exofacial ligand binding
62. Analysis of the structural features of the C-terminus of GLUT1 that are required for transport catalytic activity
63. C-terminal truncated glucose transporter is locked into an inward-facing form without transport activity
64. Cysteine scanning mutagenesis of helices 2 and 7 in GLUT1 identifies an exofacial cleft in both transmembrane segments
65. D-glucose binding increases secondary structure of human erythrocyte monosaccharide transport protein
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69. The use of biotinylation in the detection and purification of affinity labelled Glut-1
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73. QLS motif in transmembrane helix VII of the glucose transporter family interacts with the C-1 position of D-glucose and is involved in substrate selection at the exofacial binding site
74. Inhibition of 3-O-methylglucose transport in human erythrocytes by forskolin
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76. Substitution at Pro385 of GLUT1 perturbs the glucose transport function by reducing conformational flexibility
77. Localization of the forskolin photolabelling site within the monosaccharide transporter of human erythrocytes
78. Substitution of conserved tyrosine residues in helix 4 (Y143) and 7 (Y293) affects the activity, but not IAPS-forskolin binding, of the glucose transporter GLUT4
79. Mutational analysis of GLUT1 (SLC2A1) in glut-1 deficiency syndrome
80. The differential role of Cys-421 and Cys-429 of the Glut1 glucose transporter in transport inhibition by p-chloromercuribenzenesulfonic acid (pCMBS) or cytochalasin B (CB)
81. The role of cysteine residues in glucose-transporter-GLUT1-mediated transport and transport inhibition
82. Functional consequences of proline mutations in the putative transmembrane segments 6 and 10 of the glucose transporter GLUT1
83. Inability of difusion to account for placental glucose transfer in the sheep and consideration of the kinetics of a possible carrier transfer
84. Proposed structure of putative glucose channel in Glut1 facilitative glucose transporter