Assessment of poly(methacrylic acid-co-N-vinyl pyrrolidone) as a carrier for the oral delivery of therapeutic proteins using Caco-2 and HT29-MTX cell lines

Journal of Biomedical Materials Research - Part A

Volumn 92, Issue 2, 2010, Pages 504-512

  Carr, Daniel A ^a   Peppas, Nicholas A ^a,b  

^a The University of Texas at Austin   (United States)

^b UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

Cell culture; Cytocompatibility; Hydrogels; Insulin; Oral delivery

Indexed keywords

CELL LAYERS; CELL LINES; CORRESPONDING WEIGHTS; CROSSLINKED; CYTOCOMPATIBILITY; ETHYLENE GLYCOL DIMETHACRYLATE; GASTRIC CONDITIONS; LOADING EFFICIENCY; METHACRYLIC ACIDS; MICRO-PARTICLES; N-VINYL PYRROLIDONE; ORAL DELIVERY; ORAL PROTEIN DELIVERY; THERAPEUTIC AGENTS; THERAPEUTIC PROTEIN; TOXIC EFFECT;

ACIDS; ETHYLENE; ETHYLENE GLYCOL; HYDROGELS; INSULIN; PROTEINS;

CELL CULTURE;

BIOMATERIAL; DRUG CARRIER; ETHYLENE GLYCOL DIMETHACRYLATE; INSULIN; POLY(METHYLACRYLIC ACID CO N VINYL PYRROLIDONE); UNCLASSIFIED DRUG;

ARTICLE; CANCER CELL CULTURE; CELL STRAIN CACO 2; CELL STRAIN HT29; CELL VIABILITY; CONTROLLED STUDY; CROSS LINKING; DRUG DELIVERY SYSTEM; DRUG RELEASE; HUMAN; HUMAN CELL; HYDROGEL; IN VITRO STUDY; MICROPARTICLE ENZYME IMMUNOASSAY;

ADMINISTRATION, ORAL; ALGORITHMS; CACO-2 CELLS; CELL SURVIVAL; DRUG CARRIERS; DRUG DELIVERY SYSTEMS; HT29 CELLS; HUMANS; HYDROGELS; HYPOGLYCEMIC AGENTS; INSULIN; INTESTINES; KINETICS; MATERIALS TESTING; METHYLMETHACRYLATES; NANOPARTICLES; POVIDONE; PROTEINS; STOMACH;

EID: 75149193369     PISSN: 15493296     EISSN: 15524965     Source Type: Journal    
DOI: 10.1002/jbm.a.32395     Document Type: Article

References (28)

1. Nir Y, Paz A, Sabo E, Potasman I. Fear of injections in young adults: Prevalence and associations. Am J Trop Med Hyg 2003;68:341-344.
2. Partanen T-M, Rissanen A. Insulin injection practices. Pract Diab Int 2000;17:252-254.
3. Lopez JE, Peppas NA. Effect of poly (ethylene glycol) molecular weight and microparticle size on oral insulin delivery from P(MAA-g-EG) microparticles. Drug Dev Ind Pharm 2004;30:497-504.
4. Morishita M, Goto M, Takayama K, Peppas NA. Oral insulin delivery systems based on complexation polymer hydrogels. J Drug Del Sci Tech 2006;16:19-24.
5. Kim B, Peppas NA. Analysis of molecular interactions in poly (methacrylic acid-g-ethylene glycol) hydrogels. Polymer 2003; 44:3701-3707.
6. Goto T, Morishita M, Kavimandan NJ, Takayama K, Peppas NA. Gastrointestinal transit and mucoadhesive characteristics of complexation hydrogels in rats. J Pharm Sci 2006;95:462-469.
7. Nakamura K, Murray RJ, Joseph JI, Peppas NA, Morishita M, Lowman AM. Oral insulin delivery using P(MAA-g-EG) hydrogels: Effects of network morphology on insulin delivery characteristics. J Controlled Release 2004;95:589-599.
8. Besheer A, Wood KM, Peppas NA, Mader K. Loading and mobility of spin-labeled insulin in physiologically responsive complexation hydrogels intended for oral administration. J Controlled Release 2006;111:73-80.
9. Wood KM, Stone GM, Peppas NA. Wheat germ agglutinin functionalized complexation hydrogels for oral insulin delivery. Biomacromolecules 2008;9:1293-1298.
10. Moriyama K, Ooya T, Yui N. Hyaluronic acid grafted with poly(ethylene glycol) as a novel peptide formulation. J Controlled Release 1999;59:77-86.
11. Bekturov EA, Bimendina LA. Interpolymer complexes. Adv Polym Sci 1981;43:100-147.
12. Bell CL, Peppas NA. Biomedical membranes from hydrogels and interpolymer complexes. Adv Polym Sci 1995;122:125-178.
13. Blanchette J, Peppas NA. Oral chemotherapeutic delivery: Design and cellular response. Ann Biomed Eng 2005;33:142-149.
14. Ichikawa H, Peppas NA. Novel complexation hydrogels for oral peptide delivery: In vitro evaluation of their cytocompatibility and insulin-transport enhancing effects using Caco-2 cell monolayers. J Biomed Mater Res A 2003;67:609-617.
15. Torres-Lugo M, Garcia M, Record R, Peppas NA. Physicochemical behavior and cytotoxic effects of p(methacrylic acid-g-ethylene glycol) nanospheres for oral delivery of proteins. J Controlled Release 2002;80:197-205.
16. Foss AC, Peppas NA. Investigation of the cytotoxicity and insulin transport of acrylic-based copolymer protein delivery systems in contact with Caco-2 cultures. Eur J Pharm Biopharm 2004;57:447-455.
17. Orrenius S, Zhivotovsky B, Nicotera P. Regulation of cell death: The calcium-apoptosis link. Nat Rev Mol Cell Biol 2003;4:552-565.
18. Paschen W. Dependence of vital cell function on endoplasmic reticulum calcium levels: Implications for the mechanisms underlying neuronal cell injury in different pathological states. Cell Calcium 2001;29:1-11.
19. Blanchette J, Peppas NA Cellular evaluation of oral chemotherapy carriers. J Biomed Mater Res A 2005;72:381-388.
20. Madsen F, Peppas NA. Complexation graft copolymer networks: Swelling properties, calcium binding and proteolytic enzyme inhibition. Biomaterials 1999;20:1701-1708.
21. Ingels F, Deferme S, Destexhe E, Oth M, Van den Mooter G, Augustijns P. Simulated intestinal fluid as transport medium in the Caco-2 cell culture model. Int J Pharm 2002;232:183-192.
22. Hilgendorf C, Spahn-Langguth H, Regardh CG, Lipka E, Amidon GL, Langguth P. Caco-2 versus Caco-2/HT29-MTX co-cultured cell lines: Permeabilities via diffusion, inside- and outside-directed carrier-mediated transport. J Pharm Sci 2000;89:63-75.
23. Hidalgo IJ. Assessing the absorption of new pharmaceuticals. Curr Top Med Chem 2001;1:385-401.
24. Behrens I, Kissel T. Do cell culture conditions influence the carrier-mediated transport of peptides in Caco-2 cell monolayers? Eur J Pharm Sci 2003;19:433-442.
25. Balimane PV, Chong S. Cell culture-based models for intestinal permeability: A critique. Drug Discov Today 2005;10:335-343.
26. Lopez JE, Peppas NA. Cellular evaluation of insulin transmucosal delivery. J Biomater Sci Polym Ed 2004;15:385-396.
27. Kavimandan NJ, Losi E, Peppas NA. Novel delivery system based on complexation hydrogels as delivery vehicles for insulin-transferrin conjugates. Biomaterials 2006;27:3846-3854.
28. Torres-Lugo M, Garcia M, Record R, Peppas NA. pH-sensitive hydrogels as gastrointestinal tract absorption enhancers: Transport mechanisms of salmon calcitonin and other model molecules using the Caco-2 cell model. Biotechnol Prog 2002;18:612-616.