Determining Atg protein stoichiometry at the phagophore assembly site by fluorescence microscopy

Autophagy

Volumn 6, Issue 1, 2010, Pages 144-147

  Geng, Jiefei ^a   Klionsky, Daniel J ^a  

^a UNIVERSITY OF MICHIGAN   (United States)

Author keywords

Fluorescence microscopy; Lysosome; Stoichiometry; Vacuole; Yeast

Indexed keywords

AUTOPHAGY PROTEIN; HYBRID PROTEIN; MONOCLONAL ANTIBODY; PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CELL CULTURE; CENTRIFUGATION; FLUORESCENCE MICROSCOPY; FUNGAL STRAIN; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; STOICHIOMETRY; SUPERNATANT; WESTERN BLOTTING; YEAST;

EUKARYOTA;

EID: 75149118941     PISSN: 15548627     EISSN: 15548635     Source Type: Journal    
DOI: 10.4161/auto.6.1.10249     Document Type: Article

References (10)

1. Wu JQ, Pollard TD. Counting cytokinesis proteins globally and locally in fission yeast. Science 2005; 310:310-4.
2. Geng J, Baba M, Nair U, Klionsky DJ. Quantitative analysis of autophagy-related protein stoichiometry by fluorescence microscopy. J Cell Biol 2008; 182:129-40.
3. Kim J, Huang W-P, Stromhaug PE, Klionsky DJ. Convergence of multiple autophagy and cytoplasm to vacuole targeting components to a perivacuolar membrane compartment prior to de novo vesicle formation. J Biol Chem 2002; 277:763-73.
4. Suzuki K, Kirisako T, Kamada Y, Mizushima N, Noda T, Ohsumi Y. The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation. EMBO J 2001; 20:5971-81.
5. Xie Z, Nair U, Klionsky DJ. Atg8 controls phagophore expansion during autophagosome formation. Mol Biol Cell 2008; 19:3290-8.
6. He C, Song H, Yorimitsu T, Monastyrska I, Yen W-L, Legakis JE, et al. Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast. J Cell Biol 2006; 175:925-35.
7. Yen W-L, Legakis JE, Nair U, Klionsky DJ. Atg27 is required for autophagy-dependent cycling of Atg9. Mol Biol Cell 2007; 18:581-93.
8. Cheong H, Klionsky DJ. Biochemical methods to monitor autophagy-related processes in yeast. Meth Enzymol 2008; 451:1-26.
9. Ghaemmaghami S, Huh WK, Bower K, Howson RW, Belle A, Dephoure N, et al. Global analysis of protein expression in yeast. Nature 2003; 425:737-41.
10. Longtine MS, McKenzie A III, Demarini DJ, Shah NG, Wach A, Brachat A, et al. Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 1998; 14:953-61.