IscR modulates catalase a (KatA) activity, peroxide resistance, and full virulence of Pseudomonas aeruginosa PA14

Journal of Microbiology and Biotechnology

Volumn 19, Issue 12, 2009, Pages 1520-1526

  Kim, Seol Hee ^a   Lee, Bo Young ^a   Lau, Gee W ^b   Cho, You Hee ^a  

^a Sogang University   (South Korea)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

Iron; IscR; KatA; Pseudomonas aeruginosa

Indexed keywords

CATALASE; IRON SULFUR PROTEIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BACTERIAL VIRULENCE; CONTROLLED STUDY; DROSOPHILA MELANOGASTER; GENE CLUSTER; GENE DELETION; GENE EXPRESSION; GENETIC SUSCEPTIBILITY; MOUSE; NONHUMAN; PERITONITIS; PHENOTYPE; PSEUDOMONAS AERUGINOSA;

ANIMALS; CATALASE; DROSOPHILA MELANOGASTER; FEMALE; GENE DELETION; GENETIC COMPLEMENTATION TEST; HERBICIDES; HUMANS; HYDROGEN PEROXIDE; IRON; IRON-SULFUR PROTEINS; MICE; OXIDATIVE STRESS; PARAQUAT; PERITONITIS; POTASSIUM CHLORIDE; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS INFECTIONS; VIRULENCE;

BACTERIA (MICROORGANISMS); DROSOPHILA MELANOGASTER; PSEUDOMONAS AERUGINOSA;

EID: 74749093665     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.0906.06028     Document Type: Article

References (36)

1. Agar, J. N., C. Krebs, J. Frazzon, B. H. Huynh, D. R. Dean, and M. K. Johnson. 2000. IscU as a scaffold for iron-sulfur cluster biosynthesis: Sequential assembly of [2Fe-2S] and [4Fe-4S] clusters in IscU. Biochemistry 39: 7856-7862.
2. Becher, A. and H. P. Schweizer. 2000. Integration-proficient Pseudomonas aeruginosa vectors for isolation of single-copy chromosomal lacZ and lux gene fusions. Biotechniques 29: 948-950.
3. Choi, Y.-S., D.-H. Shin, I.-Y. Chung, S.-H. Kim, Y.-J. Heo, and Y.-H. Cho. 2007. Identification of Pseudomonas aeruginosa genes crucial for hydrogen peroxide resistance. J. Microbiol. Biotechnol. 17: 1344-1352.
4. D'Argenio, D. A., L. A. Gallagher, C. A. Berg, and C. Manoil. 2001. Drosophila as a model host for Pseudomonas aeruginosa infection. J. Bacteriol. 183: 1466-1471.
5. Dean, R. T., S. Fu, R. Stocker, and M. J. Davies. 1997. Biochemistry and pathology of radical-mediated protein oxidation. Biochem. J. 324: 1-18.
6. Deisseroth, A. and A. L. Dounce. 1970. Catalase: Physical and chemical properties, mechanism of catalysis, and physiological role. Physiol. Rev. 50: 319-375.
7. Ding, H. and R. J. Clark. 2004. Characterization of iron binding in IscA, an ancient iron-sulphur cluster assembly protein. Biochem. J. 379: 433-440.
8. Flint, D. H., J. F. Tuminello, and T. J. Miller. 1996. Studies on the synthesis of the Fe-S cluster of dihydroxy-acid dehydratase in Escherichia coli crude extract. Isolation of O-acetylserine sulfhydrylases A and B and beta-cystathionase based on their ability to mobilize sulfur from cysteine and to participate in Fe-S cluster synthesis. J. Biol. Chem. 271: 16053-16067.
9. 2-regulated genes in Escherichia coli. Mol. Microbiol. 60: 1058-1075.
10. Halliwell, B. and J. M. Gutteridge. 1990. Role of free radicals and catalytic metal ions in human disease: An overview. Meth. Enzymol. 186: 1-88.
11. Hassett, D. J., E. Alsabbagh, K. Parvatiyar, M. L. Howell, R. W. Wilmott, and U. A. Ochsner. 2000. A protease-resistant catalase, KatA, released upon cell lysis during stationary phase is essential for aerobic survival of a Pseudomonas aeruginosa oxyR mutant at low cell densities. J. Bacteriol. 182: 4557-4563.
12. Heo, Y. J., I.-Y. Chung, K. B. Choi, G. W. Lau, and Y.-H. Cho. 2007. Genome sequence comparison and superinfection between two related Pseudomonas aeruginosa phages, D3112 and MP22. Microbiology 153: 2885-2895.
13. Heo, Y. J., I.-Y. Chung, K. B. Choi, and Y.-H. Cho. 2007. R-Type pyocin is required for competitive growth advantage between Pseudomonas aeruginosa strains. J. Microbiol. Biotechnol. 17: 180-185.
14. Hoff, K. G., J. J. Silberg, and L. E. Vickery. 2000. Interaction of the iron-sulfur cluster assembly protein IscU with the Hsc66/Hsc20 molecular chaperone system of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 97: 7790-7795.
15. Huet, G., M. Daffe, and I. Saves. 2005. Identification of the Mycobacterium tuberculosis SUF machinery as the exclusive mycobacterial system of [Fe-S] cluster assembly: Evidence for its implication in the pathogen's survival. J. Bacteriol. 187: 6137-6146.
16. Imlay, J. A. 2006. Iron-sulphur clusters and the problem with oxygen. Mol. Microbiol. 59: 1073-1082.
17. Imlay, J. A., S. M. Chin, and S. Linn. 1998. Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro. Science 240: 640-642.
18. Kiley, P. J. and H. Beinert. 2003. Role of Fe-S clusters in sensing and regulating bacterial growth. Curr. Opin. Microbiol. 6: 181-185.
19. Krebs, C. J., N. Agar, A. D. Smith, J. Frazzon, D. R. Dean, B. H. Huynh, and M. K. Johnson. 2001. IscA, an alternate scaffold for Fe-S cluster biosynthesis. Biochemistry 40: 14069-14080.
20. Lee, J.-S., Y.-J. Heo, J.-K. Lee, and Y.-H. Cho. 2005. KatA, the major catalase, is critical for osmoprotection and virulence in Pseudomonas aeruginosa PA14. Infect. Immun. 73: 4399-4403.
21. Ma, J. F., U. A. Ochsner, M. G. Klotz, V. K. Nanayakkara, M. L. Howell, Z. Johnson, et al. 1999. Bacterioferritin A modulates catalase A (KatA) activity and resistance to hydrogen peroxide in Pseudomonas aeruginosa. J. Bacteriol. 181: 3730-3742.
22. Miller, J. H. 1972. Experiments in Molecular Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
23. Nachin, L., M. El Hassouni, L. Loiseau, D. Expert, and F. Barras. 2001. SoxR-dependent response to oxidative stress and virulence of Erwinia chrysanthemi: The key role of SufC, an orphan ABC ATPase. Mol. Microbiol. 39: 960-972.
24. Patzer, S. I. and K. Hantke. 1999. SufS is a NifS-like protein, and SufD is necessary for stability of the [2Fe-2S] FhuF protein in Escherichia coli. J. Bacteriol. 181: 3307-3309.
25. Rahme, L. G., E. J. Stevens, S. F. Wolfort, J. Shao, R. G. Tompkins, and F. M. Ausubel. 1995. Common virulence factors for bacterial pathogenicity in plants and animals. Science 268: 1899-1902.
26. Ratledge, C. and L. G. Dover. 2000. Iron metabolism in pathogenic bacteria. Annu. Rev. Microbiol. 54: 881-941.
27. Schwartz, C. J., J. L. Giel, T. Patschkowski, C. Luther, F. J. Ruzicka, H. Beinert, and P. J. Killey. 2001. IscR, an Fe-S cluster-containing transcription factor, represses expression of Escherichia coli genes encoding Fe-S cluster assembly proteins. Proc. Natl. Acad. Sci. U.S.A. 98: 14895-14900.
28. Schweizer, H. P. 1991. Escherichia-Pseudomonas shuttle vectors derived from pUC18/19. Gene 97: 109-121.
29. Scott, M. D. and J. W. Eaton. 1996. Superoxide is not the proximate cause of paraquat toxicity. Redox Report 2: 113-119.
30. Shin, D.-H., Y.-S. Choi, and Y.-H. Cho. 2008. Unusual properties of catalase A (KatA) of Pseudomonas aeruginosa PA14 are associated with its biofilm peroxide resistance. J. Bacteriol. 190: 2663-2670.
31. Sies, H. 1991. Oxidative Stress. Academic Press Ltd., Orlando, FL.
32. Smith, A. D., J. N. Agar, K. A. Johnson, J. Frazzon, I. J. Amster, D. R. Dean, and M. K. Johnson. 2001. Sulfur transfer from IscS to IscU: The first step in iron-sulfur cluster biosynthesis. J. Am. Chem. Soc. 123: 11103-11104.
33. Sonnleitner, E., S. Hagens, F. Rosenau, S. Wilhelm, A. Habel, K. E. Jager, and U. Bläsi. 2003. Reduced virulence of a hfq mutant of Pseudomonas aeruginosa O1. Microb. Pathog. 35: 217-228.
34. Tokumoto, U., S. Kitamura, K. Fukuyama, and Y. Takahashi. 2004. Interchangeability and distinct properties of bacterial Fe-S cluster assembly systems: Functional replacement of the isc and suf operons in Escherichia coli with the nifSU-like operon from Helicobacter pylori. J. Biochem. 136: 199-209.
35. Urbina, H. D., J. J. Silberg, K. G. Hoff, and L. E. Vickery. 2001. Transfer of sulfur from IscS to IscU during Fe/S cluster assembly. J. Biol. Chem. 276: 44521-44526.
36. Yeo, W.-S., J.-H. Lee, K.-C. Lee, and J.-H. Roe. 2006. IscR acts as an activator in response to oxidative stress for the suf operon encoding Fe-S assembly proteins. Mol. Microbiol. 61: 206-218.