Integration-proficient Pseudomonas aeruginosa vectors for isolation of single-copy chromosomal lacZ and lux gene fusions

BioTechniques

Volumn 29, Issue 5, 2000, Pages 948-952

  Becher, A ^a   Schweizer, H P ^a  

^a Department of Environmental and Radiological Health Sciences   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT;

ARTICLE; BACTERIOPHAGE; BIOLUMINESCENCE; CELL DENSITY; CHROMOSOME IDENTIFICATION; DNA INTEGRATION; DNA SEQUENCE; GENE CASSETTE; GENE CLUSTER; GENE FUSION; GENE SEQUENCE; GENETIC ANALYSIS; NUCLEOTIDE SEQUENCE; PROMOTER REGION; PSEUDOMONAS AERUGINOSA;

PSEUDOMONAS AERUGINOSA;

EID: 0033729216     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00295bm04     Document Type: Article

References (15)

1. Synthesis of multiple exoproducts in Pseudomonas aeruginosa is under the control of Rh1R-Rh1I, another set of regulators in strain PAO1 with homology to the autoinducer-responsive LuxR-LuxI family
2. A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: Application for isolation of unmarked Pseudomonas aeruginosa mutants
3. Integration proficient plasmids for Pseudomonas aeruginosa: Site-specific integration and use for engineering of reporter and expression strains
4. Improved vectors system for constructing transcriptional fusions that ensures independent translation of lacZ
5. Abiotic surface sensing and biofilm-dependent regulation of gene expression in Escherichia coli
6. Rapid bacterial permeabilization reagent useful for enzyme assays
7. Activation of the Pseudomonas aeruginosa lasI gene by LasR and the Pseudomonas autoinducer PAI: An autoinduction hierarchy
8. Correlation between histidine operon expression and guanosine 5'-diphosphate-3'-diphosphate levels during amino acid downshift in stringent and relaxed strains of Salmonella typhimurium
9. Uses of lac fusions for the study of biological problems
10. Improved single and multicopy lac-based cloning vectors for protein and operon fusions
11. Genetic fusions as experimental tools
12. New use of BCG for recombinant vaccines
13. Elimination of internal restriction enzyme sites from a bacterial luminescence (luxCDABE) operon
14. Improved M13 cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors