Transdentinal cytotoxic effects of different concentrations of chlorhexidine gel applied on acid-conditioned dentin substrate

Journal of Biomedical Materials Research - Part B Applied Biomaterials

Volumn 92, Issue 1, 2010, Pages 40-47

  Rosetti Lessa, Fernanda Campos ^a   Nogueira, Indri ^a   Huck, Cláudia ^a   Hebling, Josimeri ^a   De Souza Costa, Carlos Alberto ^a  

^a SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

Author keywords

Chlorhexidine; Cytotoxicity; Odontoblasts

Indexed keywords

ADHESIVE RESTORATIONS; ADHESIVE SYSTEMS; BIOLOGICAL BARRIER; CARBON POLYMERS; CELL METABOLISM; CELL MORPHOLOGY; CHEMICAL AGENT; CHLORHEXIDINE; CHLORHEXIDINE DIGLUCONATE; CONTROL GROUPS; CYTOTOXIC EFFECTS; DENTINAL TUBULES; DOSE-DEPENDENT; DOSE-DEPENDENT MANNER; ETCHED DENTIN; EXPERIMENTAL CONDITIONS; HUMAN THIRD MOLAR; PULP CHAMBERS; RESIN-DENTIN INTERFACES; RESTORATIVE DENTISTRY; STATISTICAL ANALYSIS; TOXIC EFFECT; TREATMENT GROUP;

CONCENTRATION (PROCESS); CRYSTAL ATOMIC STRUCTURE; GELS; INDUSTRIAL POISONS; METABOLISM; PHOSPHORIC ACID; RESINS; SCANNING ELECTRON MICROSCOPY;

ACIDS;

CHLORHEXIDINE GLUCONATE; HYDROXYETHYLCELLULOSE; PHOSPHORIC ACID; SALICYLIC ACID;

ARTICLE; CELL METABOLISM; CELL STRUCTURE; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; DENTIN; GEL; HUMAN; HUMAN CELL; ODONTOBLAST; SCANNING ELECTRON MICROSCOPY; THICKNESS; TOOTH PULP;

ACIDS; CELL SURVIVAL; CHLORHEXIDINE; DENTIN; GELS; HUMANS; MICROSCOPY, ELECTRON, SCANNING;

EID: 74749089532     PISSN: 15524973     EISSN: 15524981     Source Type: Journal    
DOI: 10.1002/jbm.b.31487     Document Type: Article

References (45)

1. Davies A. The mode of action of chlorhexidine. J Periodontol 1973;12:68-75.
2. Fardal O, Turnbull RS. A review of the literature on use of chlorhexidine in dentistry. J Am Dent Assoc 1986;112:863-869.
3. Hidalgo E, Dominguez C. Mechanisms underlying chlorhexidine-induced cytotoxicity. Toxicol In Vitro 2001;15:271-276.
4. Hauman CH, Love RM. Biocompatibility of dental materials used in contemporary endodontic therapy: A review. I. Intracanal drugs and substances. Int Endod J 2003;36:75-85.
5. Hugo WB, Longworth AR. The effect of chlorhexidine on the electrophoretic mobility, cytoplasmic constituents, dehydrogenase activity and cell walls of Escherichia coli and Staphylococcus aureus. J Pharm Pharmacol 1966;18:569-578.
6. Love RM, Jenkinson HF. Invasion of dentinal tubules by oral bacteria. Crit Rev Oral Biol Med 2002;13:171-183.
7. Ferraz CC, Gomes BP, Zaia AA, Teixeira FB, Souza-Filho FJ. In vitro assessment of the antimicrobial action and the mechanical ability of chlorhexidine gel as an endodontic irrigant. J Endod 2001;27:452-455.
8. de Castro FL, de Andrade MF, Duarte Júnior SL, Vaz LG, Ahid FJ. Effect of chlorhexidine on microtensile bond strength of composite to dentin. J Adhes Dent 2003;5:129-138.
9. Hebling J, Pashley DH, Tjäderhane L, Tay FR. Chlorhexidine arrests subclinical degradation of dentin hybrid layers in vivo. J Dent Res 2005;84:741-746.
10. Carrilho MR, Geraldeli S, Tay F, de Goes MF, Carvalho RM, Tjäderhane L, Reis AF, Hebling J, Mazzoni A, Breschi L, Pashley D. In vivo preservation of the hybrid layer by chlorhexidine. J Dent Res 2007;86:529-533.
11. Tjäderhane L, Larjava H, Sorsa T, Uitto VJ, Larmas M, Salo T. The activation and function of host matrix metalloproteinases in dentin matrix breakdown in caries lesions. J Dent Res 1998;77:1622-1629.
12. Gendron R, Grenier D, Sorsa T, Mayrand D. Inhibition of the activities of matrix metalloproteinases 2, 8, and 9 by chlorhexidine. Clin Diagnost Lab Immunol 1999;6:437-439.
13. Mariotti AJ, Rumpf DA. Chlorhexidine-induced changes to human gingival fibroblast collagen and non-collagen protein production. J Periodontol 1999;70:1443-1448.
14. de Souza LB, de Aquino SG, de Souza PP, Hebling J, Costa CA. Cytotoxic effects of different concentrations of chlorhexidine to the odontoblast cell line MDPC-23. Am J Dent 2007;20:400-404.
15. Faria G, Celes MR, de Rossi A, Silva LA, Silva JS, Rossi MA. Evaluation of chlorhexidine toxicity injected in the paw of mice and added to cultured l929 fibroblasts. J Endod 2007;33:715-722.
16. Hanks CT, Craig RG, Diehl ML, Pashley DH. Cytotoxicity of dental composites and other materials in a new in vitro device. J Oral Pathol 1988;17:396-403.
17. Schmalz G, Garhammer P, Schweiki H. A commercially available cell culture device modified for dentin barrier tests. J Endod 1996;22:249-252.
18. Galler K, Hiller KA, Ettl T, Schmalz G. Selective influence of dentin thickness upon cytotoxicity of dentin contacting materials. J Endod 2005;31:396-399.
19. de Souza Costa CA, do Nascimento AB, Teixeira HM. Response of human pulps following acid etching and application of bonding agent in deep cavities. Dental Mater 2002; 18:543-551.
20. Costa CA, Giro EM, do Nascimento AB, Teixeira HM, Hebling J. Short-term evaluation of the pulpo-dentin complex response to a resin-modified glass-ionomer cement and a bonding agent applied in deep cavities. Dent Mater 2003;19: 739-746.
21. Pashley DH. Smear layer: Physiological considerations. Oper Dent Suppl 1984;3:13-29.
22. Pashley DH, Carvalho RM. Dentine permeability and dentine adhesion. J Dent 1997;25:355-372.
23. Arana-Chavez VE, Massa LF. Odontoblasts: The cells forming and maintaining dentine. Int J Biochem Cell Biol 2004; 36:1367-1373.
24. Martineli ACBF. Avaliação da eficácia de agents anti-hiperestésicos: Métodos clínicos e laboratoriais. Rev Fac Odontol Bauru 2001;9:157-166.
25. Costa CA, Hanks CT. Capacity of L929 and BALB/C 3T7 fibroblasts to survive on human dentin discs. Braz Endod J 1998;3:7-13.
26. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.
27. Costa CA, Vaerten MA, Edwards CA, Hanks CT. Cytotoxic effects of current dental adhesive systems on immortalized odontoblast cell line MDPC-23. Dent Mater 1999;15:434-441.
28. Hanks CT, Wataha JC, Parsell RR, Strawn SE, Fat JC. Permeability of biological and synthetic molecules through dentine. J Oral Rehabil 1994;21:475-487.
29. Bouillaguet S, Wataha JC, Hanks CT, Ciucchi B, Holz J. In vitro cytotoxicity and dentin permeability of HEMA. J Endod 1996;22:244-248.
30. Gerzina TM, Hume WR. Diffusion of monomers from bonding resin-resin composite combinations through dentine in vitro. J Dent 1996;24:125-128.
31. Portenier I, Haapasalo H, Rye A, Waltimo T, Ørstavik D, Haapasalo M. Inactivation of root canal medicaments by dentine, hydroxylapatite and bovine serum albumin. Int Endod J 2001;34:184-188.
32. Portenier I, Haapasalo H, Orstavik D, Yamauchi M, Haapasalo M. Inactivation of the antibacterial activity of iodine potassium iodide and chlorhexidine digluconate against Enterococcus faecalis by dentin, dentin matrix, type-I collagen, and heat-killed microbial whole cells. J Endod 2002; 28:634-637.
33. Macko DJ, Rutberg M, Langeland K. Pulpal response to the application of phosphoric acid to dentin. Oral Surg Oral Med Oral Pathol 1978;45:930-946.
34. Pashley DH. The effects of acid etching on the pulpodentin complex. Oper Dent 1992;17:229-242.
35. Miyamoto T, Takahashi S, Ito H, Inagaki H, Noishiki Y. Tissue biocompatibility of cellulose and its derivatives. J Biomed Mater Res 1989;23:125-133.
36. Ferraz CC, Gomes BP, Zaia AA, Teixeira FB, Souza-Filho FJ. Comparative study of the antimicrobial efficacy of chlorhexidine gel, chlorhexidine solution and sodium hypochlorite as endodontic irrigants. Braz Dent J 2007;18:294-298.
37. Pashley DH. Consideration of dentine permeability in cytotoxicity testing. Int Endod J 1988;21:143-154.
38. Hanks CT, Fat JC, Wataha JC, Corcoran JF. Cytotoxicity and dentin permeability of carbamide peroxide and hydrogen peroxide vital bleaching materials, in vitro. Dent Res 1993; 72:931-938.
39. Gondim JO, Duque C, Hebling J, Giro EM. Influence of human dentine on the antibacterial activity of self-etching adhesive systems against cariogenic bacteria. J Dent 2008; 36:241-248.
40. Chaussain-Miller C, Fioretti F, Goldberg M, Menashi S. The role of matrix metalloproteinases (MMPs) in human caries. J Dent Res 2006;85:22-32.
41. Hashimoto M, Ohno H, Sano H, Kaga M, Oguchi H. Degradation patterns of different adhesives and bonding procedures. J Biomed Mater Res B Appl Biomater 2003;66:324-330.
42. Hashimoto M, Tay FR, Ohno H, Sano H, Kaga M, Yiu C, Kumagai H, Kudou Y, Kubota M, Oguchi H. SEM and TEM analysis of water degradation of human dentinal collagen. J Biomed Mater Res B Appl Biomater 2003;66:287-298.
43. Yoshida E, Hashimoto M, Hori M, Kaga M, Sano H, Oguchi H. Deproteinizing effects on resin-tooth bond structures. J Biomed Mater Res B Appl Biomater 2004;68:29-35.
44. Stanley HR. Biological evaluation of dental materials. Int Dent J 1992;42:37-46.
45. Schmalz G. Materials science: Biological aspects. J Dent Res 2002;81:660-663.