Cytotoxic effects of different concentrations of chlorhexidine

American Journal of Dentistry

Volumn 20, Issue 6, 2007, Pages 400-404

  De Souza, Lorena Brito ^a   De Aquino, Sabrina García ^a   De Souza, Pedro Paulo Chaves ^b   Hebling, Josimeri ^c   De Souza Costa, Carlos Alberto ^a  

^a SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

^c Department of Orthodontics and Pediatric Dentistry

Author keywords

[No Author keywords available]

Indexed keywords

BUFFER; CHLORHEXIDINE; COLORING AGENT; DIAGNOSTIC AGENT; HYDROGEN PEROXIDE; OXIDIZING AGENT; SODIUM CHLORIDE; TETRAZOLIUM; THIAZOLE DERIVATIVE; THIAZOLYL BLUE; TOPICAL ANTIINFECTIVE AGENT; UNCLASSIFIED DRUG;

ANIMAL; ARTICLE; CELL ADHESION; CELL LINE; CELL MEMBRANE; CELL SHAPE; COMPARATIVE STUDY; DOSE RESPONSE; DRUG EFFECT; METABOLISM; MOUSE; ODONTOBLAST; PATHOLOGY; ULTRASTRUCTURE;

ANIMALS; ANTI-INFECTIVE AGENTS, LOCAL; BUFFERS; CELL ADHESION; CELL LINE; CELL MEMBRANE; CELL SHAPE; CHLORHEXIDINE; COLORING AGENTS; DOSE-RESPONSE RELATIONSHIP, DRUG; HYDROGEN PEROXIDE; MICE; ODONTOBLASTS; OXIDANTS; SODIUM CHLORIDE; TETRAZOLIUM SALTS; THIAZOLES;

EID: 38349140458     PISSN: 08948275     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (32)

1. MacDougall M, Selden JK, Nydegger JR, Carnes DL. Immortalized mouse odontoblast cell line M06-G3 application for in vitro biocompatibility testing. Am J Dent 1997; 10:11-16.
2. Chaussain-Miller C, Fioretti F, Goldberg M, Menashi S. The role of matrix metalloproteinases (MMPs) in human caries. J Dent Res 2006; 85: 22-32.
3. Knott AG, Russel AD. Effects of chlorhexidine gluconate on the development of spores of Bacillus subtilis. Let Appl Microbiol 1995; 21: 117-120.
4. Wood A, Payne D. The action of three antiseptics/disinfectants against enveloped and non-enveloped viruses. J Hosp Infec 1998; 38: 283-295.
5. Sreenivasan PK, Gittins E. The effects of a chlorhexidine mouthrinse on cultivable microorganisms of the tongue and saliva. Microbiol Res 2004; 159: 365-370.
6. Theraud M, Bedouin Y, Guiguen C, Gangneux JP. Efficacy of antiseptics and disinfectants on clinical and environmental yeast isolates in planktonic and biofilm conditions. J Med Microbiol 2004; 53: 1013-1018.
7. Estrela RA, Estrela C, Reis C, Bammann LL, Pécora JD. Control of microorganisms in vitro by endodontic irrigante. Braz Dent J 2003; 14: 187-192.
8. Jeansonne MJ, White RR. A comparison of 2% chlorhexidine gluconate and 5.25% sodium hypochlorite as antimicrobial endodontic irrigants. J Endod 1994; 20: 276-278.
9. White RR, Hais GL, Janer LR. Residual antimicrobial activity after canal irrigation with chlorhexidine. J Endod 1997; 24: 229-231.
10. Fure S, Emilson CG. Effect of chlorhexidine gel treatment supplemented with chlorhexidine varnish and resin on mutans streptococci and Actinomyces on root surfaces. Caries Res 1990; 24: 242-247.
11. Emilson CG. Susceptibility of various microorganisms to chlorhexidine. Scand J Dent Res 1997; 85: 255-265.
12. Basrani B, Santos JM, Tjäderhane L, Grad H, Gorduysus O, Huang J, Lawrence HP, Friedman S. Substantive antimicrobial activity in chlorhexidine-treated human root dentin. Oral Surg Oral Med Oral Pathol 2002; 94: 240-245.
13. Cervone F, Tronstad L, Hammond B. Antimicrobial effect of chlorhexidine in a controlled release delivery system. Endod Dent Traumatol 1990; 6: 33-36.
14. de Castro FL, de Andrade MF, Duarte-Junior SL, Vaz LG, Ahid FJ. Effect of chlorhexidine on microtensile bond strength of composite to dentin. J Adhes Dent 2003; 5: 129-138.
15. Gendron R, Grenier D, Sorsa T, Mayrand D. Inhibition of the activities of matrix metalloproteinases 2, 8, and 9 by chlorhexidine. Clin Diag Lab Immunol 1999; 6: 437-439.
16. Tjäderhane L, Larjava H, Sorsa T, Uitto VJ, Larmas M, Salo T. The activation and function of host matrix metalloproteinases in dentin matrix breakdown in caries lesions. J Dent Res 1998; 77: 1622-1629.
17. Hebling J, Pashley DH, Tjäderhane L, Tay FR. Chlorhexidine arrests subclinical degradation of dentin hybrid layers in vivo. J Dent Res 2005; 84: 741-746.
18. Helgeland K, Heyden G, Rölla G. Effect of chlorhexidine on animals cells in vitro. Scand J Dent Res 1971; 79: 209-215.
19. Babich H, Würzburger BJ, Rubin YL, Sinensky MC. Blau L. An in vitro study on the cytotoxicity of chlorhexidine digluconate to human gingival cells. Cell Biol Toxicol 2005; 11: 79-88.
20. Chang YC, Huang FM, Tai KW. The effect of sodium hypochlorite and chlorhexidine on cultured human periodontal ligament cells. Oral Surg Oral Med Oral Pathol 2001; 92: 446-450.
21. Hidalgo E, Dominguez C. Mechanisms underlying chlorhexidine-induced cytotoxicity. Toxicol in Vitro 2001; 15: 271-276.
22. Hanks CT, Sun ZL, Fang DN, Edwards CA, Wataha JC, Ritchie HH, Butler WT. Cloned 3T6 cell line from CD-1 mouse fetal molar dental papillae. Connect Tissue Res 1998; 37: 1-17.
23. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65: 55-63.
24. Goldschmidt T, Cogen R, Taubman S. Cytopathologic effects of chlorhexidine on human cells. J Periodontal 1977; 48: 212-215.
25. Pashley DH, Tay FR, Yiu C, Hashimoto M, Breschi L, Carvalho RM, Ito S. Collagen degradation by host-derived enzymes during aging. J Dent Res 2004; 83: 216-221.
26. Schmalz G, Schweikl H. Characterization of an in vitro dentin barrier test using standard toxicant. J Endod 1994; 20: 592-594.
27. Evans, MD, Baumgartner JC, Khemaleelakul S, Xia T. Efficacy of calcium hydroxide: Chlorhexidine paste as an intracanal medication in bovine dentin. J Endod 2003; 29: 338-339.
28. Lin S, Zuckerman O, Waiss EI, Mazor I, Fuss Z. Antibacterial efficacy of a chlorhexidine slow release device to disinfect dentinal tubules. Int Endod J 2003, 11th Biennial Congress ESE, Athens, Greece: 32.
29. Costa CAS, Giro EMA, do Nascimento AB, Teixeira HM, Hebling J. Short-term evaluation of the pulp-dentin complex response to a resinmodified glass-ionomer cement and a bonding agent applied in deep cavities. Dent Mater 2003; 19: 739-746.
30. Spencer P, Wang Y. Adhesive phase separation at the dentin interface under wet bonding conditions. J Biomed Mater Res 2002; 62: 447-456.
31. Costa CAS, Hebling J, Randall RC. Human pulp response to resin cements used to bond inlay restorations. Dent Mater 2006;22:954-962.
32. Costa CAS, Nascimento ABL, Teixeira HM. Response of human pulps following acid conditioning and application of bonding agent in deep cavities. Dent Mater 2002; 18: 543-551.