Photochemically knocking out glutamate receptors in vivo

Journal of the American Chemical Society

Volumn 126, Issue 43, 2004, Pages 13886-13887

  Chambers, James J ^a   Gouda, Hiroaki ^a   Young, David M ^a   Kuntz, Irwin D ^a   England, Pamela M ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLUTAMATE RECEPTOR;

ANIMAL CELL; ARTICLE; CATALYSIS; CRYSTAL STRUCTURE; NONHUMAN; OOCYTE; PHOTOCHEMISTRY; PHOTOLYSIS; ULTRAVIOLET RADIATION; VOLTAGE CLAMP; XENOPUS;

ANIMALS; EXCITATORY AMINO ACID ANTAGONISTS; NEURONS; NITRO COMPOUNDS; PHOTOCHEMISTRY; QUINOLINES; QUINOXALINES; RECEPTORS, AMPA; SYNAPSES; ULTRAVIOLET RAYS; XENOPUS;

EID: 7444248510     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja048331p     Document Type: Article

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26. The apparent cross-linking efficiency of ANQX in these experiments was likely limited by the size (∼1 mm diameter), shape (spherical), and opacity of the oocytes. In preliminary experiments on cultured neurons expressing endogenous AMPARs, irradiation in the presence of ANQX completely inactivated AMPAR currents after only 2 s of photolysis and with significantly less UV light (data not shown).
27. 2) for ANQX predicts that ANQX is membrane impermeable. Finally, the photochemical knock-out experiments require only brief (<1 min) exposure to ANQX - a period too short to permit penetration of this compound through the plasma membrane.