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2-terminal domain of α- and β-SNAP (designed to disrupt the protein-protein interaction of SNAPs) was QSFFSGLFGGSSKIEEACE (26). The peptide was amidified on the COOH-terminal and purified by reverse phase high-pressure liquid chromatography.
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17
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15144356992
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note
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The scrambled S19 peptide, which was prepared in the same way as N19, was GFAESLFQSIEKESGFSCG (26).
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The botulinum toxin was heated to 90°C for 30 min, and its solubility after denaturation was checked by spectrophotometry.
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It should be noted that while some reduction in the potentiation was evident shortly after the tetanus in the presence of inhibitors of membrane fusion, this potentiation decayed over a 10-to 30-min period. There are a number of possible explanations for this remaining short-term potentiation (STP), which is of longer duration than posttetanic potentiation. One possibility, which we do not favor, is that this STP is mechanistically distinct from LTP. Alternatively, the blockade of membrane fusion may not be complete, and multiple fusion events may be required to stabilize the potentiation.
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15144356394
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Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
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44
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note
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We thank H. Czerwonka for secretarial assistance. P.-M.L. was supported by CNRS and by a North Atlantic Treaty Organization fellowship. R.A.N. is a member of the Keck Center for Integrative Neurobiology and the Silvio Conte Center for Neuroscience Research. R.C.M. is a member of the Center for Neurobiology and Psychiatry and the Center for the Neurobiology of Addiction. R.A.N. is supported by grants from the NIH. R.C.M. is supported by grants from the NIH, the Human Frontier Science Program, and the McKnight Endowment Fund for Neuroscience.
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