Generation of human embryonic stem cell reporter knock-in lines by homologous recombination

Current Protocols in Stem Cell Biology

Volumn , Issue SUPPL.11, 2009, Pages

  Davis, Richard P ^a,b   Grandela, Catarina ^a,c   Sourris, Koula ^a   Hatzistavrou, Tanya ^a   Dottori, Mirella ^d   Elefanty, Andrew G ^a   Stanley, Edouard G ^a   Costa, Magdaline ^a  

^a MONASH UNIVERSITY   (Australia)

^b LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^c ACADEMIC MEDICAL CENTER   (Netherlands)

^d UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

Fluorescent reporter gene; Gene targeting; HESCs; Homologous recombination; Human embryonic stem cells

Indexed keywords

CRE RECOMBINASE; GENOMIC DNA;

CELL ISOLATION; DNA FLANKING REGION; ELECTROPORATION; EMBRYONIC STEM CELL; ENRICHMENT CULTURE; FLOW CYTOMETRY; GENE CASSETTE; GENE LOCUS; GENE TARGETING; GENE VECTOR; GENETIC REGULATION; GENETIC SELECTION; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN EMBRYO; KNOCKOUT GENE; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REPORTER GENE; REVIEW; ARTICLE; BIOLOGICAL MODEL; CULTURE TECHNIQUE; CYTOLOGY; GENE EXPRESSION REGULATION; GENETIC PROCEDURES; GENETIC RECOMBINATION; GENETICS; INSTRUMENTATION; METHODOLOGY; TRANSGENE;

CELL CULTURE TECHNIQUES; ELECTROPORATION; EMBRYONIC STEM CELLS; FLOW CYTOMETRY; GENES, REPORTER; GENETIC TECHNIQUES; GENETIC VECTORS; HUMANS; MODELS, GENETIC; MUTAGENESIS, INSERTIONAL; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; TRANSGENES;

EID: 73949151642     PISSN: 19417322     EISSN: 19388969     Source Type: Journal    
DOI: 10.1002/9780470151808.sc05b01s11     Document Type: Review

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