Improved genetic manipulation of human embryonic stem cells

Nature Methods

Volumn 5, Issue 5, 2008, Pages 389-392

  Braam, Stefan R ^a,b   Denning, Chris ^c   van den Brink, Stieneke ^a   Kats, Peter ^d   Hochstenbach, Ron ^d   Passier, Robert ^a,b   Mummery, Christine L ^a,b,e  

^a HUBRECHT INSTITUTE   (Netherlands)

^b LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^c UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^d UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

^e ROYAL NETHERLANDS ACADEMY OF ARTS AND SCIENCES   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOVIRUS VECTOR; CELL SURFACE MARKER; LENTIVIRUS VECTOR; LIPOFECTAMINE; OCTAMER TRANSCRIPTION FACTOR 4; PLASMID DNA; SMALL INTERFERING RNA; TRANSCRIPTION FACTOR SOX2;

ARTICLE; CELL ASSAY; CELL CULTURE; CELL DENSITY; CELL DIFFERENTIATION; CELL GROWTH; CELL LINE; CELL TRANSFER; CONTROLLED STUDY; CULTURE TECHNIQUE; ELECTROPORATION; EMBRYO; EMBRYONIC STEM CELL; FLOW CYTOMETRY; GENE EXPRESSION; GENE FUNCTION; GENE TRANSFER; GENETIC MANIPULATION; GENETIC SCREENING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; INTERMETHOD COMPARISON; KARYOTYPE; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; TRANSGENE; VIRAL GENE DELIVERY SYSTEM;

CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINE; COLLAGEN; DNA-BINDING PROTEINS; DRUG COMBINATIONS; EMBRYONIC STEM CELLS; FIBROBLASTS; GENE SILENCING; GENE TRANSFER TECHNIQUES; HMGB PROTEINS; HUMANS; LAMININ; PROTEOGLYCANS; REPRODUCIBILITY OF RESULTS; RNA, SMALL INTERFERING; TIME FACTORS; TRANSCRIPTION FACTORS; TRYPSIN;

EID: 42949088949     PISSN: 15487091     EISSN: 15491676     Source Type: Journal    
DOI: 10.1038/nmeth.1200     Document Type: Article

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