In vivo metabolic tracking of 14C-radiolabelled isoflavones in kudzu (Pueraria lobata) and red clover (Trifolium pratense) extracts

British Journal of Nutrition

Volumn 102, Issue 10, 2009, Pages 1523-1530

  Mun, Jonathan G ^a   Grannan, Michael D ^b   Lachcik, Pamela J ^b   Reppert, Adam ^a   Yousef, Gad G ^a   Rogers, Randy B ^a   Janle, Elsa M ^b   Weaver, Connie M ^b   Lila, Mary Ann ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b PURDUE UNIVERSITY   (United States)

Author keywords

Kudzu; Pharmacokinetics; Radiolabelling; Red clover

Indexed keywords

BIOCHANIN A; CARBON 14; DAIDZIN; FORMONONETIN; GENISTEIN; GLYCOSIDE; ISOFLAVONE DERIVATIVE; PUERARIA EXTRACT; PUERARIN; RED CLOVER EXTRACT;

ANIMAL EXPERIMENT; ARTICLE; BLOOD ANALYSIS; BONE TISSUE; CONTROLLED STUDY; DRUG ABSORPTION; DRUG ACCUMULATION; DRUG DISTRIBUTION; DRUG ELIMINATION; DRUG FECES LEVEL; DRUG METABOLISM; DRUG URINE LEVEL; ENTEROHEPATIC CIRCULATION; FECES ANALYSIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IN VIVO STUDY; INTERSTITIAL FLUID; ISOTOPE LABELING; MALE; MASS SPECTROMETRY; NONHUMAN; PUERARIA; RADIOACTIVITY; RAT; RED CLOVER; URINALYSIS;

ANIMALS; CARBON; CARBON RADIOISOTOPES; CELLS, CULTURED; EXTRACELLULAR FLUID; ISOFLAVONES; MALE; PLANT ROOTS; PUERARIA; RATS; RATS, SPRAGUE-DAWLEY; TIME FACTORS; TRIFOLIUM;

PUERARIA MONTANA VAR. LOBATA; RATTUS; TRIFOLIUM; TRIFOLIUM PRATENSE;

EID: 73749088373     PISSN: 00071145     EISSN: 14752662     Source Type: Journal    
DOI: 10.1017/S000711450999047X     Document Type: Article

References (30)

1. Jacobs MN & Lewis DFV (2002) Steroid hormone receptors and dietary ligands: a selected review. Proc Nutr Soc 61, 105-122.
2. Genant H, Lucas J, Weiss S, et al. (1997) Low-dose esterified estrogen therapy: effects on bone, plasma estradiol concentrations, endometrium, and lipid levels. Arch Intern Med 157, 2609-2615.
3. Kaufman PB, Duke JA, Brielmann H, et al. (1997) A comparative survey of leguminous plants as sources of the isoflavones, genistein and daidzein: implications for human nutrition and health. J Altern Complement Med 3, 7-12.
4. Swinny EE & Ryan KG (2005) Red clover Trifolium pratense L. phytoestrogens: UV-B radiation increases isoflavone yield, and postharvest drying methods change the glucoside conjugate profiles. J Agric Food Chem 53, 8273-8278.
5. Zhang Y, Zeng X, Zhang L, et al. (2007) Stimulatory effect of puerarin on bone formation through activation of PI3K/ Akt pathway in rat calvaria osteoblasts. Planta Med 73, 341-347.
6. Wong R & Rabie B (2007) Effect of puerarin on bone formation. Osteoarthritis Cartilage 15, 894-899.
7. Urasopon N, Hamada Y, Cherdshewasart W, et al. (2008) Preventive effects of Pueraria mirifica on bone loss in ovariectomized rats. Maturitas 59, 137-148.
8. Wang X, Wu J, Chiba H, et al. (2003) Puerariae radix prevents bone loss in ovariectomized mice. J Bone Miner Metab 21, 268-275.
9. Clifton-Bligh PB, Baber RJ, Fulcher GR, et al. (2001) The effect of isoflavones extracted from red clover (Rimostil) on lipid and bone metabolism. Menopause 8, 259-265.
10. Atkinson C, Compston JE, Day NE, et al. (2004) The effects of phytoestrogen isoflavones on bone density in women: a doubleblind, randomized, placebo-controlled trial. Am J Clin Nutr 79, 326-333.
11. Grusak MA (1997) Intrinsic stable isotope labeling of plants for nutritional investigations in humans. J Nutr Biochem 8, 164-171.
12. Grusak MA, Rogers RB, Yousef GG, et al. (2004) An enclosedchamber labeling system for the safe 14C-enrichment of phytochemicals in plant cell suspension cultures. In Vitro Cell Dev Biol Plant 40, 80-85.
13. Yousef GG, Seigler DS, Grusak MA, et al. (2004) Biosynthesis and characterization of 14C-enriched flavonoid fractions from plant cell suspension cultures. J Agric Food Chem 52, 1138-1145.
14. Lila MA, Yousef GG, Jiang Y, et al. (2005) Sorting out bioactivity in flavonoid mixtures. J Nutr 135, 1231-1235.
15. Chandra A, Rana J & Li Y (2001) Separation, identification, quantification, and method validation of anthocyanins in botanical supplement raw materials by HPLC and HPLC-MS. J Agric Food Chem 49, 3515-3521.
16. Murashige T & Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15, 473-497.
17. Rogers RB & Smith MAL (1992) Consequences of in vitro and ex vitro root initiation for miniature rose production. J Hort Sci 67, 535-540.
18. Hakamatsuka T, Ebizuka Y & Sankawa U (1994) Pueraria lobata (kudzu vine): in vitro culture and the production of isoflavonoids. vol.28, Biotechnology in Agriculture and Forestry, Medicinal and Aromatic Plants VII, pp. 386-400 [YPS Bajai, editor]. Berlin, Germany: Springer-Verlag.
19. Beach KH & Smith RR (1979) Plant regeneration from callus of red and crimson clover. Plant Sci Lett 16, 231-237.
20. Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50, 151-158.
21. Engelmann N, Rogers RB, Vattem P, et al. (2006) Copper chloride elicitation of in vitro red clover isoflavones. J Soc In Vitro Biol 42, 34A.
22. Fernandez LA, Rettori O & Mejia RH (1966) Correlation between body fluid volumes and body weight in the rat. Am J Physiol 210, 877-879.
23. Reppert A, Yousef GG, Rogers RB, et al. (2008) Isolation of radiolabeled isoflavones from kudzu (Pueraria lobata) root cultures. J Agric Food Chem 56, 7860-7865.
24. Prasain JK, Reppert A, Jones K, et al. (2007) Identification of isoflavone glycosides in Pueraria lobata cultures by tandem mass spectrometry. Phytochem Anal 18, 50-59.
25. Engelmann NJ, Reppert A, Yousef G, et al. (2009) In vitro production of radiolabeled red clover (Trifolium pratense) isoflavones. Plant Cell Tissue Organ Cult (epublication ahead of print version 26 May 2009).
26. Prasain JK, Peng N, Moore R, et al. (2009) Tissue distribution of puerarin and its conjugated metabolites in rats assessed by liquid chromatography-tandem mass spectrometry. Phytomedicine 16, 65-71.
27. Janning P, Schuhmacher US, Upmeier A, et al. (2000) Toxicokinetics of the phytoestrogen daidzein in female DA/Han rats. Arch Toxicol 74, 421-430.
28. Sfakianos J, Coward L, Kirk M, et al. (1997) Intestinal uptake and biliary excretion of the isoflavone genistein in rats. J Nutr 127, 1260-1268.
29. Occhiuto F, De Pasquale R, Guglielmo G, et al. (2007) Effects of phytoestrogenic isoflavones from red clover (Trifolium pratense L.) on experimental osteoporosis. Phytother Res 21, 130-134.
30. Prasain JK, Jones K, Brissie N, et al. (2004) Identification of puerarin and its metabolites in rats by liquid chromatography- tandem mass spectrometry. J Agric Food Chem 52, 3708-3712.