Methods for rapid identification of a functional single-chain variable fragment using alkaline phosphatase fusion

BMB Reports

Volumn 42, Issue 11, 2009, Pages 731-736

  Lee, Kyung Woo ^a   Hur, Byung Ung ^a   Song, Suk Yoon ^a   Choi, Hyo Jung ^a   Shin, Sang Hoon ^a   Cha, Sang Hoon ^a  

^a Kangwon National University   (South Korea)

Author keywords

Alkaline phosphatase; BCKD E2; Hybridoma; PhoA; ScFv

Indexed keywords

ALKALINE PHOSPHATASE; IMMUNOGLOBULIN FRAGMENT; PRIMER DNA;

ANIMAL; ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENETICS; IMMUNOHISTOCHEMISTRY; MOLECULAR CLONING; MOUSE; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION;

ALKALINE PHOSPHATASE; ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; IMMUNOGLOBULIN FRAGMENTS; IMMUNOHISTOCHEMISTRY; MICE; POLYMERASE CHAIN REACTION;

EID: 73449144139     PISSN: 19766696     EISSN: 1976670X     Source Type: Journal    
DOI: 10.5483/BMBRep.2009.42.11.731     Document Type: Article

References (28)

1. Morino, K., Katsumi, H., Akahori, Y., Iba, Y., Shinohara, M., Ukai, Y., Kohara, Y. and Kurosawa, Y. (2001) Antibody fusions with fluorescent proteins: a versatile reagent for profiling protein expression. J. Immunol. Methods 257, 175-184.
2. Huston, J. S., McCartney, J., Tai, M. S., Mottola-Hartshorn, C., Jin, D., Warren, F., Keck, P. and Oppermann, H. (1993) Medical applications of single-chain antibodies. Intern. Rev. Immunol. 10, 195-217.
3. Winter, G., Griffiths, A. D., Hawkins, R. E. and Hoogenboom, H. R. (1994) Making antibodies by phage display technology. Annu. Rev. Immunol. 12, 433-455.
4. Vaughan, T. J., Williams, A. J., Pritchard, K., Osbourn, J. K., Pope, A. R., Earnshaw, J. C., McCafferty, J., Hodits, R. A., Wilton, J. and Johnson, K. S. (1996) Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library. Nature. Biotechnol. 14, 309-314.
5. Xiang, J., Zhong, Z., Deng, N., Zhong, Z. and Yang, H. (2005) Screening of the Antigen epitopes of basic fibroblast growth factor by phage display. BMB Reports 38, 290-293.
6. Nicholls, P. J., Johnson, V. G., Blanford, M. D. and Andrew, S. M. (1993) An improved method for generating single-chain antibodies from hybridomas. J. Immunol. Methods 165, 81-91.
7. Kütemeier, G., Harloff, C. and Mocikat, R. (1992) Rapid isolation of immunoglobulin variable genes from cell lysates of rat hybridomas by polymerase chain reaction. Hybridoma 11, 23-32.
8. Hua, X., Yuliang R., Jinliang, X., Xiangmin, Y., Yn, L. and Zhinan, C. (2005) Expression vectors for human-mouse chimeric antibodies. BMB Reports 38, 414-419.
9. Krebber, A., Bornhauser, S., Burmester, J., Honegger, A., Willuda, J., Bosshard, H. R. and Pluckthun, A. (1997) Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system. J. Immunol. Methods 201, 35-55.
10. Yu, Z. C., Ding, J., Nie, Y. Z., Fan, D. M. and Zhang, X. Y. (2001) Preparation of single chain variable fragment of MG (7) mAb by phage display technology. World J. Gastroenterol. 7, 510-514.
11. Rodenburg, C. M., Mernaugh, R., Bilbao, G. and Khazaeli, M. B. (1998) Production of a single chain anti-CEA antibody from the hybridoma cell line T84.66 using a modified colony-lift selection procedure to detect antigen-positive ScFv bacterial clones. Hybridoma 17, 1-8.
12. Courtney, B. C., Williams, K. C. and Schlager, J. J. (1995) A phage display vector with improved stability, applicability and ease of manipulation. Gene 165, 139-140.
13. McCafferty, J. (1996) Phage display: factors affecting panning efficiency; in Phage Display of Peptides and Proteins, a Laboratory Manual, Kay, B. K., Winter, J. and Mc Cafferty, J. (eds.), pp. 261-276, Academic Press, San Diego, USA.
14. Oh, M., Joo, H., Hur, B., Jeong, Y. and Cha, S. (2007) Enhancing phage display of antibody fragments using gIII-amber suppression. Gene 386, 81-89.
15. Skerra, A., Dreher, M. L. and Winter G. (1991) Filter screening of antibody Fab fragments secreted from individual bacterial colonies: specific detection of antigen binding with a two-membrane system. Anal. Biochem. 196, 151-155.
16. Dreher, M. L., Gherardi, E., Skerra, A. and Milstein, C. (1991) Colony assays for antibody fragments expressed in bacteria. J. Immunol. Methods 139, 197-205.
17. Suzuki, C., Ueda, H., Suzuki, E. and Nagamune, T. (1997) Construction, bacterial expression, and characterization of hapten-specific single-chain Fv and alkaline phosphatase fusion protein. J. Biochem. 122, 322-329.
18. Rau, D., Kramer, K. and Hock, B. (2002) Single-chain Fv antibody-alkaline phosphatase fusion proteins produced by one-step cloning as rapid detection tools for ELISA. J. Immunoassay Immunochem. 23, 129-143.
19. Fregeau, D. R., Davis, P. A., Danner, D. J., Ansari, A., Coppel, R. L., Dickson, E. R. and Gershwin, M. E. (1989) Antimitochondrial antibodies of primary biliary cirrhosis recognize dihydrolipoamide acyltransferase and inhibit enzyme function of the branched chain alpha-ketoacid dehydrogenase complex. J. Immunol. 142, 3815-3820.
20. Kipriyanov, S. M., Kupriyanova, O. A., Little, M. and Moldenhauer, G. (1996) Rapid detection of recombinant antibody fragments directed against cell-surface antigens by flow cytometry. J. Immunol. Methods 196, 51-62.
21. Mousli, M., Goyffon, M. and Billiald, P. (1998) Production and characterization of a bivalent single chain Fv/alkaline phosphatase conjugate specific for the hemocyanin of the scorpion Androctonus australis. Biochim. Biophys. Acta. 1425, 348-360.
22. Yamanaka, H. I., Kirii, Y. and Ohmoto, H. (1995) An improved phage display antibody cloning system using newly designed PCR primers optimized for Pfu DNA polymerase. J. Biochem. 117, 1218-1227.
23. Martin, C. D., Rojas, G., Mitchell, J. N., Vincent, K. J., Wu, J., McCafferty, J. and Schofield, D. J. (2006) A simple vector system to improve performance and utilisation of recombinant antibodies. BMC Biotechnol. 6, 46.
24. Gardberg, A. S., Dice, L. T., Ou, S., Rich, R. L., Helmbrecht, E., Ko, J., Wetzel, R., Myszka, D. G., Patterson, P. H. and Dealwis, C. (2007) Molecular basis for passive immunotherapy of Alzheimer's disease. Proc. Natl. Acad. Sci. U.S.A. 104, 15659-15664.
25. Collet, T. A., Roben, P., O'Kennedy, R., Barbas, C. F. 3rd, Burton, D. R. and Lerner, R. A. (1992) A binary plasmid system for shuffling combinatorial antibody libraries. Proc. Natl. Acad. Sci. U.S.A. 89, 10026-10030.
26. Kim, D., Kim, S., An, J., Lee, S., Jang, S., Won, M., Kang, T., Chung, K., Jung, H., Cho, S., Choi, J., Park, J., Eum, W. and Choi, S. (2006) Expression, Purification and Transduction of PEP-1-Botulinum Neurotoxin Type A (PEP-1-BoNT/A) into Skin. BMB Reports 39, 642-647.
27. Choi, S., Kim S., An, J., Lee, S., Kim, D., Won, M., Kang, T., Park, J., Eum, W., Kim, J. and Choi, S. (2006) Immunohistochemical studies of human ribosomal protein S3 (rpS3). BMB Reports 39, 208-215.
28. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed., Cold spring Harbor Laboratory Press, New York, USA.