Expression vectors for human-mouse chimeric antibodies

Journal of Biochemistry and Molecular Biology

Volumn 38, Issue 4, 2005, Pages 414-419

  Xiong, Hua ^a   Ran, Yuliang ^b   Xing, Jinliang ^a   Yang, Xiangmin ^a   Li, Yu ^a   Chen, Zhinan ^a  

^a FOURTH MILITARY MEDICAL UNIVERSITY   (China)

^b PEKING UNION MEDICAL COLLEGE   (China)

Author keywords

Antibody engineering; Chimeric antibody; Chinese hamster ovary cells; Eucaryotic expression; Expression vector

Indexed keywords

CRICETULUS GRISEUS; EUKARYOTA; MAMMALIA; MURINAE;

EID: 29844439234     PISSN: 12258687     EISSN: 12258687     Source Type: Journal    
DOI: 10.5483/bmbrep.2005.38.4.414     Document Type: Article

References (22)

1. Burton, D. R. (1991) Human and mouse monoclonal antibodies by repertoire cloning. Trends Biotechnol. 9, 169-175.
2. Bianchi, A. A. and McGrew, J. T. (2003) High-level expression of full-length antibodies using trans-complementing expression vectors. Biotechnol. Bioeng. 84, 439-444.
3. Fouser, L. A., Swanberg, S. L., Lin, B. Y., Benedict, M., Kelleher K., Cumming D. A. and Riedel, G. E. (1992) High level expression of a chimeric anti-ganglioside GD2 antibody: Genomic κ sequences improve expression in COS and CHO cells. Bio/Technology 10, 1121-1127.
4. Gorman, S. D., Clark, M. R., Routledge, E. G., Cobbold, S. P. and Waldmann, H. (1991) Reshaping a therapeutic CD4 antibody. Proc. Natl. Acad. Sci. USA 88, 4181-4185.
5. Harris, W. J. and Emery, S. (1993) Therapeutic antibodies: the coming of age. Trends Biotechnol. 11, 42-44.
6. Kaufman, R. J., Sharp, P. A. and Latt, S. A. (1983) Evolution of chromosomal regions containing transfected and amplified dihydrofolate reductase sequences. Mol. Cell. Biol 3, 699-711.
7. Kim, N. S., Byun, T. H. and Lee, G. M. (2001) Key determinants in the occurrence of clonal variation in humanized antibody expression of CHO cells during dihydrofolate reductase mediated gene amplification. Biotechnol Prog. 17, 69-75.
8. Kim, S. J. (1998) Characterization of chimeric antibody producing CHO cells in the course of dihydrofolate reductase-mediated gene amplification and their stability in the absence of selective pressure. Biotechnol. Bioeng. 58, 73-84.
9. Kim, S. J. and Lee, G. M. (1999) Cytogenetic analysis of chimeric antibody-producing CHO cells in the course of dihydrofolate reductase-mediated gene amplification and their stability in the absence of selective pressure. Biotechnol. Bioeng. 64, 741-749.
10. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
11. Liu, A. Y., Robinson, R. R., Hellstrom, K. E., Murray, E. D., Chang, C. P. and Hellstrom, I. (1987) Chimeric mouse-human IgG1 antibody that can mediate lysis of cancer cells. Proc. Natl. Acad. Sci. USA 84, 3439-3443.
12. Liu, W. T. and Wei, H. M. (2004) A balanced expression of two chains of heterodimer protein, the human interleukin-12, improves high-level expression of the protein in CHO cells. Biochem. Biophys. Res. Commun. 313, 287-293.
13. Mclean, G. R. and Antonio, N. (2000) Human and murine immunoglobulin expression vector cassettes. Mol. Immunol. 37, 837-845.
14. Morrison, S. L. (1985) Transfectomas provide novel chimeric antibodies. Science 229, 1202.
15. Newman, R., Alberts, J., Anderson, D., Carner, K., Heard, C., Norton, F., Raab, R., Reef, M., Shuey, S. and Hanna N. (1992) Primatization of recombinant antibodies for immunotherapy of human diseases: A macaque/human chimeric against human CD4. Bio/Technology 10, 1455-1460.
16. Page, M. J. and Sydenham, M. A. (1991) High level expression of the humanized monoclonal antibody Campath-1H in Chinese hamster ovary cells. Bio/Technology 9, 64-68.
17. Persic, L., Roberts A., Wliton J., Cattaneo, A., Bradbury, A. and Hoogenboom, H. R. (1997) An integrated vector system for the eukaryotic expression of antibodies or their fragments after selection from phage display libraries. Gene 187, 9-18.
18. Tada, H., Kurokawa, T., Seita, T., Watanabe, T. and Iwasa, S. (1994) Expression and characterization of a chimeric bispecific antibody against fibrin and against urokinase-type plasminogen activator. J. Biotechnol. 33, 157-174.
19. Trill, J. J. (1995) Production of monoclonal antibodies in COS and CHO cells. Curr. Opin. Biotechnol. 6, 553-560.
20. Waldmann, T. A. (1991) Monoclonal antibodies in diagnosis and therapy. Science 252, 1657-1662.
21. Wood, C. R., Dorner, A. J., Morris, G. E., Alderman, E. M., Wilson, D., O'hara, R. M. and Kaufman, R. J. (1990) High level synthesis of immunoglobulins in Chinese hamster ovary cells. J. Immunol. 145, 3011-3016.
22. Yang, X. M., Xing, J. L., Yao, X. Y., Zhang, S. H. and Chen, Z. N. (2004) The cloning and expression of Fab gene of MAb CAb-1 against human colorectal cancer. J. Fourth Military Med. Univ. 25, 1768-1771.