Construction, bacterial expression, and characterization of hapten-specific single-chain Fv and alkaline phosphatase fusion protein

Journal of Biochemistry

Volumn 122, Issue 2, 1997, Pages 322-329

  Suzuki, Chikako ^a   Ueda, Hiroshi ^a   Suzuki, Eiji ^a   Nagamune, Teruyuki ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

Alkaline phosphatase; Antibody engineering; Fusion protein; NP; Single chain Fv

Indexed keywords

3 NITROPHENOL; ALKALINE PHOSPHATASE; ANTIBODY CONJUGATE; HAPTEN; IMMUNOGLOBULIN F(AB) FRAGMENT; MONOCLONAL ANTIBODY;

AFFINITY CHROMATOGRAPHY; ARTICLE; BINDING AFFINITY; BINDING KINETICS; DIMERIZATION; ENZYME BINDING; ENZYME KINETICS; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME PURIFICATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; EXPRESSION VECTOR; GEL FILTRATION; GENE FUSION; IMMUNOGLOBULIN VARIABLE REGION; MOLECULAR WEIGHT; NONHUMAN; PROTEIN ANALYSIS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; FELIS CATUS;

EID: 0030765096     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021756     Document Type: Article

References (31)

1. Halow, E. and Lane, D.P. (1988) Antibodies. A Laboratory Manual, Cold Spring Harbor Laboratory Press, New York
2. Ternynck, T., Portsmann, T., and Avrameas, S. (1987) Enzyme immunoassay using a monoclonal antibody against 5-bromo-2-deoxyuridine for the assessment of lymphoid cell proliferation. Methods Enzymol. 150, 117-129
3. Ducancel, F., Gillet, D., Carrier, A., Lajeunesse, E., Menez, A., and Boulain, J.C. (1993) Recombinant colorimetric antibodies: construction and characterization of a bifunctional F(ab)2/alkaline phosphatase conjugate produced in Escherichia coli. Biotechnology 11, 601-605
4. Weiss, E., Chatellier, J., and Orfanoudakis, G. (1994) In vivo biotinylated recombinant antibodies: construction, characterization, and application of a bifunctional Fab-BCCP fusion protein produced in Escherichia coli. Protein Exp. Purif. 5, 509-517
5. Wang, C.L., Huang, M., Wesson, C.A., Birdsell, D.C., and Trumble, W.R. (1994) A single Fc binding domain - alkaline phosphatase gene fusion expresses a protein with both IgG binding ability and alkaline phosphatase enzymatic activity. Protein Eng. 7, 715-722
6. Nakayama, T., Arata, Y., and Shimada, I. (1993) A multinuclear NMR study of the affinity maturation of anti-NP mouse monoclonal antibodies: comparison of antibody combining sites of primary response antibody N1G9 and secondary response antibody 3B62. Biochemistry 32, 13961-13968
7. Mizutani, R., Miura, K., Nakayama, T., Shimada, I., Arata, Y., and Satow, Y. (1995) Three-dimensional structures of the Fab fragment of murine N1G9 antibody from the primary immune response and of its complex with (4-hydroxy-3-nitrophenyl) acetate. J. Mol. Biol. 254, 208-222
8. Ueda, H., Kikuchi, M., Yagi, S., and Nishimura, H. (1992) Antigen responsive antibody-receptor kinase chimera. Biotechnology 10, 430-433
9. Williams, G.T. and Neuberger, M.S. (1986) Production of antibody-tagged enzymes by myeloma cells: application to DNA polymerase I Klenow fragment. Gene 43, 319-324
10. Casadei, J., Powell, M.J., and Kenten, J.H. (1990) Expression and secretion of aequorin as a chimeric antibody by means of a mammalian expression vector. Proc. Natl. Acad. Sci. USA 87, 2047-2051
11. Bird, R.E., Hardman, K.D., Jacobson, J.W., Johnson, S., Kaufman, B.M., Lee, S.M., Lee, T., Pope, S.H., Riordan, G.S., and Whitlow, M. (1988) Single-chain antigen-binding proteins [published erratum appears in Science 1989 Apr 28;244(4903): 409]. Science 242, 423-426
12. Huston, J.S., Levinson, D., Mudgett-Hunter, M., Tai, M.S., Novotny, J., Margolies, M.N., Ridge, R.J., Bruccoleri, R.E., Haber, E., Crea, R. et al. (1988) Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proc. Natl. Acad. Sci. USA 85, 5879-5883
13. Raag, R. and Whitlow, M. (1995) Single-chain Fvs. FASEB J. 9, 73-80
14. Brinkmann, U., Lee, B.K., and Pastan, I. (1993) Recombinant immunotoxins containing the VH or VL domain of monoclonal antibody B3 fused to Pseudomonas exotoxin. J. Immunol. 150, 2774-2782
15. Goshorn, S.C., Svensson, H.P., Kerr, D.E., Somerville, J.E., Senter, P.D., and Fell, H.P. (1993) Genetic construction, expression, and characterization of a single chain anti-carcinoma antibody fused to beta-lactamase. Cancer Res. 53, 2123-2127
16. Pantoliano, M.W., Bird, R.E., Johnson, S., Asel, E.D., Dodd, S.W., Wood, J.F., and Hardman, K.D. (1991) Conformational stability, folding, and ligand-binding affinity of single-chain Fv immunoglobulin fragments expressed in Escherichia coli. Biochemistry 30, 10117-10125
17. Oi, V.T., Morrison, S.L., Herzenberg, L.A., and Berg, P. (1983) Immunoglobulin gene expression in transformed lymphoid cells. Proc. Natl. Acad. Sci. USA 80, 825-829
18. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning Ver. 2. A Laboratory Manual, Cold Spring Harbor Laboratory Press, New York.
19. Studier, F.W. (1991) Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system. J. Mol. Biol. 219, 37-44
20. Tyler-Cross, R., Roberts, C.H., and Chlebowski, J.F. (1989) Proteolytic modification of Escherichia coli alkaline phosphatase. J. Biol. Chem. 264, 4523-4528
21. Azuma, T., Sakato, N., and Fujio, H. (1987) Maturation of the immune response to (4-hydroxy-3-nitrophenyl)-acetyl (NP) haptens in C57BL/6 mice. Mol. Immunol. 24, 287-296
22. Gettins, P. and Coleman, J.E. (1983) 31P nuclear magnetic resonance of phosphoenzyme intermediates of alkaline phosphatase. J. Biol. Chem. 258, 408-416
23. Kim, E.E. and Wyckoff, H.W. (1991) Reaction mechanism of alkaline phosphatase based on crystal structures. Two-metal ion catalysis. J. Mol. Biol. 218, 449-464
24. Jones, P.T., Dear, P.H., Foote, J., Neuberger, M.S., and Winter, G. (1986) Replacing the complementarity-determining regions in a human antibody with those from a mouse. Nature 321, 522-525
25. Wels, W., Harwerth, I.M., Zwickl, M., Hardman, N., Groner, B., and Hynes, N.E. (1992) Construction, bacterial expression and characterization of a bifunctional single-chain antibody-phosphatase fusion protein targeted to the human erbB-2 receptor. Biotechnology 10, 1128-1132
26. Sun, S. and Lew, A.M. (1992) Chimaeric protein A/protein G and protein G/alkaline phosphatase as reporter molecules. J. Immunol. Methods 152, 43-48
27. Reiter, Y., Brinkmann, U., Lee, B., and Pastan, I. (1996) Engineering antibody Fv fragments for cancer detection and therapy: Disulfide-stabilized Fv fragments. Biotechnology 14, 1239-1245
28. Davis, G.T., Bedzyk, W.D., Voss, E.W., and Jacobs, T.W. (1991) Single chain antibody (SCA) encoding genes: one-step construction and expression in eukaryotic cells. Biotechnology 9, 165-169
29. Tsumoto, K., Nakaoki, Y., Ueda, Y., Ogasahara, K., Yutani, K., Watanabe, K., and Kumagai, I. (1994) Effect of the order of antibody variable regions on the expression of the single-chain HyHEL10 Fv fragment in E. coli and the thermodynamic analysis of its antigen-binding properties. Biochem. Biophys. Res. Commun. 201, 546-551
30. Bedzyk, W.D., Weidner, K.M., Denzin, L.K., Johnson, L.S., Hardman, K.D., Pantoliano, M.W., Asel, E.D., and Voss, E.W., Jr. (1990) Immunological and structural characterization of a high affinity anti-fluorescein single-chain antibody. J. Biol. Chem. 265, 18615-18620
31. Grodberg, J. and Dunn, J.J. (1988) ompT encodes the Escherichia coli outer membrane protease that cleaves T7 Rna polymerase during purification. J. Bacteriol. 170, 1245-1253