Emodin protects rat liver from CCl4-induced fibrogenesis via inhibition of hepatic stellate cells activation

World Journal of Gastroenterology

Volumn 15, Issue 38, 2009, Pages 4753-4762

  Dong, Miao Xian ^a   Jia, Yan ^b   Zhang, Ying Bo ^a   Li, Cheng Chong ^a   Geng, Yu Tao ^a   Zhou, Li ^a   Li, Xue Yan ^a   Liu, Ji Cheng ^a   Niu, Ying Cai ^a  

^a QIQIHAR MEDICAL UNIVERSITY   (China)

^b HEILONGJIANG UNIVERSITY OF CHINESE MEDICINE   (China)

Author keywords

Emodin; Hepatic fibrosis; Hepatic stellate cell; Smad4; Transforming growth factor 1; smooth muscle actin

Indexed keywords

ALANINE AMINOTRANSFERASE; ALPHA SMOOTH MUSCLE ACTIN; ASPARTATE AMINOTRANSFERASE; CARBON TETRACHLORIDE; EMODIN; EOSIN; HEMATOXYLIN; HYDROXYPROLINE; MESSENGER RNA; OLIVE OIL; SMAD4 PROTEIN; TRANSFORMING GROWTH FACTOR BETA1;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BIOCHEMISTRY; CELL ACTIVATION; CONTROLLED STUDY; DOWN REGULATION; DRUG MECHANISM; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; FIBROGENESIS; HISTOPATHOLOGY; IMMUNOHISTOCHEMISTRY; INHIBITION KINETICS; LIVER CELL; LIVER FIBROSIS; LIVER INJURY; LIVER PROTECTION; MALE; MICROSCOPY; NONHUMAN; PROTEIN EXPRESSION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STAINING; STELLATE CELL; WESTERN BLOTTING;

EID: 73449105395     PISSN: 10079327     EISSN: None     Source Type: Journal    
DOI: 10.3748/wjg.15.4753     Document Type: Article

References (46)

1. Farci P, Roskams T, Chessa L, Peddis G, Mazzoleni AP, Scioscia R, Serra G, Lai ME, Loy M, Caruso L, Desmet V, Purcell RH, Balestrieri A. Long-term benefit of interferon alpha therapy of chronic hepatitis D: regression of advanced hepatic fibrosis. Gastroenterology 2004; 126: 1740-1749
2. Satapathy SK, Sakhuja P, Malhotra V, Sharma BC, Sarin SK. Beneficial effects of pentoxifylline on hepatic steatosis, fibrosis and necroinflammation in patients with non-alcoholic steatohepatitis. J Gastroenterol Hepatol 2007; 22: 634-638
3. Seki E, De Minicis S, Osterreicher CH, Kluwe J, Osawa Y, Brenner DA, Schwabe RF. TLR4 enhances TGF-beta signaling and hepatic fibrosis. Nat Med 2007; 13: 1324-1332
4. Decologne N, Kolb M, Margetts PJ, Menetrier F, Artur Y, Garrido C, Gauldie J, Camus P, Bonniaud P. TGF-beta1 induces progressive pleural scarring and subpleural fibrosis. J Immunol 2007; 179: 6043-6051
5. Yao Q, Pawlaczyk K, Ayala ER, Styszynski A, Breborowicz A, Heimburger O, Qian JQ, Stenvinkel P, Lindholm B, Axelsson J. The role of the TGF/Smad signaling pathway in peritoneal fibrosis induced by peritoneal dialysis solutions. Nephron Exp Nephrol 2008; 109: e71-e78
6. Yang Y, Yang S, Chen M, Zhang X, Zou Y, Zhang X. Compound Astragalus and Salvia miltiorrhiza Extract exerts anti-fibrosis by mediating TGF-beta/Smad signaling in myofibroblasts. J Ethnopharmacol 2008; 118: 264-270
7. De Minicis S, Seki E, Uchinami H, Kluwe J, Zhang Y, Brenner DA, Schwabe RF. Gene expression profiles during hepatic stellate cell activation in culture and in vivo. Gastroenterology 2007; 132: 1937-1946
8. Cheng K, Mahato RI. Gene modulation for treating liver fibrosis. Crit Rev Ther Drug Carrier Syst 2007; 24: 93-146
9. Yang YC, Lim MY, Lee HS. Emodin isolated from Cassia obtusifolia (Leguminosae) seed shows larvicidal activity against three mosquito species. J Agric Food Chem 2003; 51: 7629-7631
10. Yim H, Lee YH, Lee CH, Lee SK. Emodin, an anthraquinone derivative isolated from the rhizomes of Rheum palmatum, selectively inhibits the activity of casein kinase II as a competitive inhibitor. Planta Med 1999; 65: 9-13
11. Guo JM, Xiao BX, Liu Q, Zhang S, Liu DH, Gong ZH. Anticancer effect of aloe-emodin on cervical cancer cells involves G2/M arrest and induction of differentiation. Acta Pharmacol Sin 2007; 28: 1991-1995
12. Shuangsuo D, Zhengguo Z, Yunru C, Xin Z, Baofeng W, Lichao Y, Yan'an C. Inhibition of the replication of hepatitis B virus in vitro by emodin. Med Sci Monit 2006; 12: BR302-BR306
13. Chang CH, Lin CC, Yang JJ, Namba T, Hattori M. Anti-inflammatory effects of emodin from ventilago leiocarpa. Am J Chin Med 1996; 24: 139-142
14. Zhan Y, Li D, Wei H, Wang Z, Huang X, Xu Q, Lu H. Emodin on hepatic fibrosis in rats. Chin Med J (Engl) 2000; 113: 599-601
15. Zhan YT, Liu B, Li DG, Bi CS. [Mechanism of emodin for anti-fibrosis of liver] Zhonghua Ganzangbing Zazhi 2004; 12: 245-246
16. Proctor E, Chatamra K. High yield micronodular cirrhosis in the rat. Gastroenterology 1982; 83: 1183-1190
17. Abe W, Ikejima K, Lang T, Okumura K, Enomoto N, Kitamura T, Takei Y, Sato N. Low molecular weight heparin prevents hepatic fibrogenesis caused by carbon tetrachloride in the rat. J Hepatol 2007; 46: 286-294
18. [Guidelines for diagnosis and treatment of nonalcoholic fatty liver diseases] Zhonghua Ganzangbing Zazhi 2006; 14: 161-163
19. Nouchi T, Worner TM, Sato S, Lieber CS. Serum procollagen type III N-terminal peptides and laminin P1 peptide in alcoholic liver disease. Alcohol Clin Exp Res 1987; 11: 287-291
20. Kim KM, Choi WB, Park SH, Yu E, Lee SG, Lim YS, Lee HC, Chung YH, Lee YS, Suh DJ. Diagnosis of hepatic steatosis and fibrosis by transient elastography in asymptomatic healthy individuals: a prospective study of living related potential liver donors. J Gastroenterol 2007; 42: 382-388
21. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001; 25: 402-408
22. Bialek, Redd JT, Lynch A, Vogt T, Lewis S, Wilson C, Bell BP. Chronic liver disease among two American Indian patient populations in the southwestern United States, 2000-2003. J Clin Gastroenterol 2008; 42: 949-954
23. Adrian JE, Poelstra K, Scherphof GL, Meijer DK, van Loenen-Weemaes AM, Reker-Smit C, Morselt HW, Zwiers P, Kamps JA. Effects of a new bioactive lipid-based drug carrier on cultured hepatic stellate cells and liver fibrosis in bile duct-ligated rats. J Pharmacol Exp Ther 2007; 321: 536-543
24. Prud'homme GJ. Pathobiology of transforming growth factor beta in cancer, fibrosis and immunologic disease, and therapeutic considerations. Lab Invest 2007; 87: 1077-1091
25. Song M, Song Z, Barve S, Zhang J, Chen T, Liu M, Arteel GE, Brewer GJ, McClain CJ. Tetrathiomolybdate protects against bile duct ligation-induced cholestatic liver injury and fibrosis. J Pharmacol Exp Ther 2008; 325: 409-416
26. Pihusch V, Pihusch M, Penovici M, Kolb HJ, Hiller E, Pihusch R. Transforming growth factor beta-1 released from platelets contributes to hypercoagulability in veno-occlusive disease following hematopoetic stem cell transplantation. Thromb Res 2005; 116: 233-240
27. Zou YH, Yang Y, Li J, Wu Q, Li WP, Lu JT, Roberts MS. Potential therapeutic effects of a traditional Chinese formulation, BJ-JN, on liver fibrosis induced by carbon tetrachloride in rats. J Ethnopharmacol 2008; 120: 452-457
28. Yuan LP, Chen FH, Ling L, Bo H, Chen ZW, Li F, Zhong MM, Xia LJ. Protective effects of total flavonoids of Bidens bipinnata L. against carbon tetrachloride-induced liver fibrosis in rats. J Pharm Pharmacol 2008; 60: 1393-1402
29. You S, Zhou M, Xue B, Fang T, Jiang W, Li C, Xu H, Jiang J, Wang Y, Xu S. A clinical study on bing gan ling oral liquid for treatment of hepatitis C. J Tradit Chin Med 1998; 18: 209-214
30. Weiler-Normann C, Herkel J, Lohse AW. Mouse models of liver fibrosis. Z Gastroenterol 2007; 45: 43-50
31. Smyth R, Munday MR, York MJ, Clarke CJ, Dare T, Turton JA. Comprehensive characterization of serum clinical chemistry parameters and the identification of urinary superoxide dismutase in a carbon tetrachloride-induced model of hepatic fibrosis in the female Hanover Wistar rat. Int J Exp Pathol 2007; 88: 361-376
32. Petlevski R, Hadzija M, Bajalo JL, Juretić D. Effect of acarbose on alanine aminotransferase and aspartate aminotransferase activities in the liver of control and diabetic CBA mice. Acta Pharm 2006; 56: 87-93
33. Tajima K, Terai S, Takami T, Kawaguchi K, Okita K, Sakaida I. Importance of inhibitor of DNA binding/differentiation 2 in hepatic stellate cell differentiation and proliferation. Hepatol Res 2007; 37: 647-655
34. Tu CT, Guo JS, Wang M, Wang JY. Antifibrotic activity of rofecoxib in vivo is associated with reduced portal hypertension in rats with carbon tetrachloride-induced liver injury. J Gastroenterol Hepatol 2007; 22: 877-884
35. Luedde T, Trautwein C. A molecular link between inflammation and fibrogenesis: the bacterial microflora influences hepatic fibrosis via toll-like receptor 4-dependent modification of transforming growth factor-beta signaling in hepatic stellate cells. Hepatology 2008; 47: 1089-1091
36. Mochizuki M, Shimizu S, Urasoko Y, Umeshita K, Kamata T, Kitazawa T, Nakamura D, Nishihata Y, Ohishi T, Edamoto H. Carbon tetrachloride-induced hepatotoxicity in pregnant and lactating rats. J Toxicol Sci 2009; 34: 175-181
37. Chou WY, Lu CN, Lee TH, Wu CL, Hung KS, Concejero AM, Jawan B, Wang CH. Electroporative interleukin-10 gene transfer ameliorates carbon tetrachloride-induced murine liver fibrosis by MMP and TIMP modulation. Acta Pharmacol Sin 2006; 27: 469-476
38. Kottler UB, Jünemann AG, Aigner T, Zenkel M, Rummelt C, Schlötzer-Schrehardt U. Comparative effects of TGF-beta 1 and TGF-beta 2 on extracellular matrix production, proliferation, migration, and collagen contraction of human Tenon's capsule fibroblasts in pseudoexfoliation and primary open-angle glaucoma. Exp Eye Res 2005; 80: 121-134
39. Xu Q, Norman JT, Shrivastav S, Lucio-Cazana J, Kopp JB. In vitro models of TGF-beta-induced fibrosis suitable for high-throughput screening of antifibrotic agents. Am J Physiol Renal Physiol 2007; 293: F631-F640
40. Nakamuta M, Morizono S, Tsuruta S, Kohjima M, Kotoh K, Enjoji M. Remote delivery and expression of soluble type II TGF-beta receptor in muscle prevents hepatic fibrosis in rats. Int J Mol Med 2005; 16: 59-64
41. Ma LJ, Jha S, Ling H, Pozzi A, Ledbetter S, Fogo AB. Divergent effects of low versus high dose anti-TGF-beta antibody in puromycin aminonucleoside nephropathy in rats. Kidney Int 2004; 65: 106-115
42. Tang Y, Katuri V, Srinivasan R, Fogt F, Redman R, Anand G, Said A, Fishbein T, Zasloff M, Reddy EP, Mishra B, Mishra L. Transforming growth factor-beta suppresses nonmetastatic colon cancer through Smad4 and adaptor protein ELF at an early stage of tumorigenesis. Cancer Res 2005; 65: 4228-4237
43. Hariharan R, Pillai MR. Structure-function relationship of inhibitory Smads: Structural flexibility contributes to functional divergence. Proteins 2008; 71: 1853-1862
44. Dai P, Nakagami T, Tanaka H, Hitomi T, Takamatsu T. Cx43 mediates TGF-beta signaling through competitive Smads binding to microtubules. Mol Biol Cell 2007; 18: 2264-2273
45. Chu GC, Dunn NR, Anderson DC, Oxburgh L, Robertson EJ. Differential requirements for Smad4 in TGFbeta-dependent patterning of the early mouse embryo. Development 2004; 131: 3501-3512
46. Maurice D, Pierreux CE, Howell M, Wilentz RE, Owen MJ, Hill CS. Loss of Smad4 function in pancreatic tumors: C-terminal truncation leads to decreased stability. J Biol Chem 2001; 276: 43175-43181