Modification of N-glycosylation sites allows secretion of bacterial chondroitinase ABC from mammalian cells

Journal of Biotechnology

Volumn 145, Issue 2, 2010, Pages 103-110

  Muir, Elizabeth M ^a   Fyfe, Ian ^a   Gardiner, Sonya ^a   Li, Li ^a   Warren, Philippa ^a   Fawcett, James W ^b   Keynes, Roger J ^a   Rogers, John H ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Chondroitinase; Endoplasmic reticulum; Glycosylation; Protein secretion; Spinal cord injury

Indexed keywords

BACTERIAL CELLS; BACTERIAL PROTEIN; CENTRAL NERVOUS SYSTEMS; CHONDROITINASE; ENDOPLASMIC RETICULUM; EUKARYOTIC CELLS; EUKARYOTIC PROTEINS; FUNCTIONAL ENZYMES; GENE THERAPY; GLYCOSYLATED; HUMAN SPINAL CORD; MAMMALIAN CELLS; N-GLYCOSYLATION; N-GLYCOSYLATION SITES; NEURAL REGENERATION; POTENTIAL TOOL; PROTEIN SECRETION; PROTEOGLYCANS; PROTEUS VULGARIS; SECRETORY PATHWAYS; SPINAL CORD INJURY; TRANSLATION SYSTEMS;

BACTERIOLOGY; DOSIMETRY; ESTERIFICATION; GENETIC ENGINEERING; MAMMALS; PATIENT REHABILITATION; PROTEIN FOLDING;

GLYCOSYLATION;

CHONDROITIN ABC LYASE;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BACTERIUM TRANSDUCTION; CELL BASED GENE THERAPY; CONTROLLED STUDY; DRUG SCREENING; ENZYME ACTIVITY; ENZYME MODIFICATION; ENZYME RELEASE; GENETIC TRANSFECTION; GLYCATION; IN VITRO STUDY; MAMMAL CELL; NERVE DEGENERATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEUS VULGARIS; RAT; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CELLS, CULTURED; CHONDROITIN ABC LYASE; CLONING, MOLECULAR; ENZYME ACTIVATION; ENZYME STABILITY; GLYCOSYLATION; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEUS VULGARIS; RABBITS; RECOMBINANT PROTEINS; RETICULOCYTES;

BACTERIA (MICROORGANISMS); EUKARYOTA; MAMMALIA; PROKARYOTA; PROTEUS VULGARIS;

EID: 72949093996     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.11.002     Document Type: Article

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