Structural characterization of N-linked oligosaccharides of Defibrase from Agikistrodon acutus by sequential exoglycosidase digestion and MALDI-TOF mass spectrometry

Toxicon

Volumn 55, Issue 2-3, 2010, Pages 421-429

  Luo, Xiaoqing ^a,c   Yang, Huaxin ^b   Liang, Chenggang ^b   Jin, Shaohong ^b  

^a CHINA PHARMACEUTICAL UNIVERSITY   (China)

^b NATIONAL INSTITUTE FOR THE CONTROL OF PHARMACEUTICAL AND BIOLOGICAL PRODUCTS   (China)

^c Suzhou Health College   (China)

Author keywords

Defibrase; MALDI TOF MS; Oligosaccharides structure; Sequential exoglycosidase digestion

Indexed keywords

2 AMINOBENZAMIDE; ASPARAGINE LINKED OLIGOSACCHARIDE; BATROXOBIN; FLUORESCENT DYE; FUCOSE; GALACTOSE; MANNOSE; MONOSACCHARIDE; N ACETYLGLUCOSAMINE; SIALIC ACID; SNAKE VENOM;

AGIKISTRODON ACUTUS; AMPEROMETRY; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CARBOHYDRATE ANALYSIS; FRACTIONATION; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; METHYLATION; NONHUMAN; PRIORITY JOURNAL; SEQUENTIAL ANALYSIS; SNAKE;

AGKISTRODON; ANIMALS; BATROXOBIN; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CROTALID VENOMS; ELECTROCHEMISTRY; FIBRINOLYTIC AGENTS; FLUORESCENT DYES; GLYCOSIDE HYDROLASES; HYDROLYSIS; METHYLATION; MONOSACCHARIDES; OLIGOSACCHARIDES; OXIDATION-REDUCTION; SIALIC ACIDS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

DEINAGKISTRODON ACUTUS;

EID: 72749103896     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxicon.2009.09.007     Document Type: Article

References (27)

1. Castro H.C., Zingali R.B., Albuquerque M.G., Pujol-Luz M., and Rodrigues C.R. Snake venom thrombin-like enzymes: from reptilase to now. Cell Mol. Life Sci. 61 7-8 (2004) 843-856
2. Fares F. The role of O-linked and N-linked oligosaccharides on the structure-function of glycoprotein hormones: development of agonists and antagonists. Biochim. Biophys. Acta Gen. Subj. 1760 4 (2006) 560-567
3. García L.T., Parreiras E Silva L.T., Ramos O.H.P., Carmona A.K., Bersanetti P.A., and Selistre-De-Araujo H.S. The effect of post-translational modifications on the hemorrhagic activity of snake venom metalloproteinases. Comp. Biochem. Physiol. C, Comp. Pharmacol. Toxicol. 138 1 (2004) 23-32
4. Geyer H., and Geyer R. Strategies for analysis of glycoprotein glycosylation. Biochim. Biophys. Acta. 1764 12 (2006) 1853-1869
5. Gowda D.C., Jackson C.M., Kurzban G.P., McPhie P., and Davidson E.A. Core sugar residues of the N-linked oligosaccharides of Russell's viper venom factor X-activator maintain functionally active polypeptide structure. Biochemistry 35 18 (1996) 5833-5837
6. Gowda D.C., Schultz M., Bredehorst R., and Vogel C.W. Structure of the major oligosaccharide of cobra venom factor. Mol. Immunol. 29 3 (1992) 335-342
7. Gowda D.C., Jackson C.M., Hensley P., and Davidson E.A. Factor X-activating glycoprotein of Russell's viper venom. Polypeptide composition and characterization of the carbohydrate moieties. J. Biol. Chem. 269 14 (1994) 10644-10650
8. Harvey D.J. Proteomic analysis of glycosylation: structural determination of N- and O-linked glycans by mass spectrometry. Expert Rev. Proteomics 2 1 (2005) 87-101
9. Helenius A., and Aebi M. Intracellular functions of N-linked glycans. Science 291 5512 (2001) 2364-2369
10. Helenius A., and Aebi M. Roles of N-linked glycans in the endoplasmic reticulum. Annu. Rev. Biochem. 73 (2004) 1019-1049
11. Hodgson W.C., and Isbister G.K. The application of toxins and venoms to cardiovascular drug discovery. Curr. Opin. Pharmacol. 9 2 (2009) 173-176
12. Kannicht C., Grunow D., and Lucka L. Enzymatic sequence analysis of N-glycans by exoglycosidase cleavage and mass spectrometry - detection of lewis X structures. Methods Mol. Biol. 446 (2008) 255-266
13. Kini R.M. Anticoagulant proteins from snake venoms: structure, function and mechanism. Biochem. J. 397 3 (2006) 377-387
14. Koh D.C.I., Armugam A., and Jeyaseelan K. Snake venom components and their applications in biomedicine. Cell Mol. Life Sci. 63 24 (2006) 3030-3041
15. Ku ster B., Naven T.J.P., and Harvey D.J. Rapid approach for sequencing neutral oligosaccharides by exoglycosidase digestion and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J. Mass Spectrom. 31 10 (1996) 1131-1140
16. Marsh N., and Williams V. Practical applications of snake venom toxins in haemostasis. Toxicon 45 8 (2005) 1171-1181
17. Morelle W., Canis K., Chirat F., Faid V., and Michalski J.C. The use of mass spectrometry for the proteomic analysis of glycosylation. Proteomics 6 14 (2006) 3993-4015
18. Ouyang C., and Teng C.M. The effect of the purified anticoagulant principle of Agkistrodon acutus venom on blood coagulation. Toxicon 11 3 (1973) 287-292
19. Ouyang C., and Teng C.M. In vivo effects of the purified thrombin-like and anticoagulant principles of Agkistrodon acutus (Hundred pace snake) venom. Toxicon 16 6 (1978) 583-593
20. Oyama, E., H. Takahashi. 2003. Purification and characterization of a thrombin like enzyme, elegaxobin II, with lys-bradykinin releasing activity from the venom of Trimeresurus elegans (Sakishima-Habu). Toxicon 41(5), 559-568.
21. Pan H., Du X., Yang G., Zhou Y., and Wu X. cDNA cloning and expression of acutin, a thrombin-like enzyme from Agkistrodon acutus. Biochem. Bioph. Res. Co. 255 2 (1999) 412-415
22. Pfeiffer G., Dabrowski U., Dabrowski J., Stirm S., Strube K.H., and Geyer R. Carbohydrate structure of a thrombin-like serine protease from Agkistrodon rhodostoma. Structure elucidation of oligosaccharides by methylation analysis, liquid secondary-ion mass spectrometry and proton magnetic resonance. Eur. J. Biochem. 205 3 (1992) 961-978
23. Sant'Ana C.D., Bernardes C.P., Izidoro L.F.M., Mazzi M.V., Soares S.G., Fuly A.L., Zingali R.B., Magro A.J., Braz A.S.K., Fontes M.R.M., Sta beli R.G., Sampaio S.V., and Soares A.M. Molecular characterization of BjussuSP-I, a new thrombin-like enzyme with procoagulant and kallikrein-like activity isolated from Bothrops jararacussu snake venom. Biochimie 90 3 (2008) 500-507
24. Satomi Y., Shimonishi Y., and Takao T. N-glycosylation at Asn491 in the Asn-Xaa-Cys motif of human transferrin. Febs Lett. 576 1-2 (2004) 51-56
25. Silva-Junior F.P., Guedes H.L.M., Garvey L.C., Aguiar A.S., Bourguignon S.C., Di Cera E., and Giovanni-De-Simone S. BJ-48, a novel thrombin-like enzyme from the Bothrops jararacussu venom with high selectivity for Arg over Lys in P1: role of N-glycosylation in thermostability and active site accessibility. Toxicon 50 1 (2007) 18-31
26. Vance B.A., Wu W., Ribaudo R.K., Segal D.M., and Kearse K.P. Multiple dimeric forms of human CD69 result from differential addition of N-glycans to typical (Asn-X-Ser/Thr) and atypical (Asn-X-Cys) glycosylation motifs. J. Biol. Chem. 272 37 (1997) 23117-23122
27. Zingali R.B. Interaction of snake-venom proteins with blood coagulation factors: mechanisms of anticoagulant activity. J. Toxicol. Toxin. Rev. 26 1 (2007) 25-46