The Sca2 autotransporter protein from Rickettsia conorii is sufficient to mediate adherence to and invasion of cultured mammalian cells

Infection and Immunity

Volumn 77, Issue 12, 2009, Pages 5272-5280

  Cardwell, Marissa M ^a   Martinez, Juan J ^a  

^a UNIVERSITY OF CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; CARRIER PROTEIN; OUTER MEMBRANE PROTEIN; OUTER MEMBRANE PROTEIN A; OUTER MEMBRANE PROTEIN B; SURFACE CELL ANTIGEN 2; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BACTERIAL GENOME; BACTERIAL VIRULENCE; BACTERIUM ADHERENCE; BACTERIUM CULTURE; BIOINFORMATICS; CONTROLLED STUDY; ENDOTHELIUM CELL; ENZYME ASSAY; ESCHERICHIA COLI; GENETIC TRANSCRIPTION; HETEROLOGOUS EXPRESSION; HOST CELL; HUMAN; HUMAN CELL; MAMMAL CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; OUTER MEMBRANE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN TRANSPORT; RICKETTSIA CONORII; RICKETTSIACEAE INFECTION;

ADHESINS, BACTERIAL; ANIMALS; BACTERIAL ADHESION; BACTERIAL PROTEINS; CELL LINE; ESCHERICHIA COLI; GENE EXPRESSION PROFILING; HUMANS; MODELS, BIOLOGICAL; RICKETTSIA CONORII; VIRULENCE FACTORS;

EID: 72449133297     PISSN: 00199567     EISSN: 10985522     Source Type: Journal    
DOI: 10.1128/IAI.00201-09     Document Type: Article

References (22)

1. Blanc, G., M. Ngwamidiba, H. Ogata, P. E. Fournier, J. M. Claverie, and D. Raoult. 2005. Molecular evolution of Rickettsia surface antigens: evidence of positive selection. Mol. Biol. Evol. 20:2073-2083.
2. Chan, Y. G. Y., M. M. Cardwell, T. M. Hermanas, T. Uchiyama, and J. J. Martinez. 2009. Rickettsial outer-membrane protein B (rOmpB) mediates bacterial invasion through Ku70 in an actin, c-Cbl, clathrin and caveolin 2-dependent manner. Cell. Microbiol. 11:629-644.
3. Cossart, P., and P. J. Sansonetti. 2004. Bacterial invasion: the paradigms of enteroinvasive pathogens. Science 304:242-248. (Pubitemid 38452169)
4. Edgell, C. J., C. C. McDonald, and J. B. Graham. 1983. Permanent cell line expressing human factor VIII-related antigen established by hybridization. Proc. Natl. Acad. Sci. USA 80:3734-3737.
5. Feng, H. M., T. Whitworth, J. P. Olano, V. L. Popov, and D. H. Walker. 2004. Fc-dependent polyclonal antibodies and antibodies to outer membrane proteins A and B, but not to lipopolysaccharide, protect SCID mice against fatal Rickettsia conorii infection. Infect. Immun. 72:2222-2228.
6. Feng, H. M., T. Whitworth, V. Popov, and D. H. Walker. 2004. Effect of antibody on the Rickettsia-host cell interaction. Infect. Immun. 72:3524-3530.
7. Gouin, E., H. Gantelet, C. Egile, I. Lasa, H. Ohayon, V. Villiers, P. Gounon, P. J. Sansonetti, and P. Cossart. 1999. A comparative study of the actin-based motilities of the pathogenic bacteria Listeria monocytogenes, Shigella flexneri and Rickettsia conorii. J. Cell Science 112:1697-1708.
8. Hackstadt, T. 1996. The biology of rickettsiae. Infect. Agents Dis. 5:127-143.
9. Hackstadt, T., R. Messer, W. Cieplak, and M. G. Peacock. 1992. Evidence for proteolytic cleavage of the 120-kilodalton outer membrane protein of rickettsiae: identification of an avirulent mutant deficient in processing. Infect. Immun. 60:159-165.
10. Hand, W. L., J. B. Miller, J. A. Reinarz, and J. P. Sanford. 1970. Rocky Mountain spotted fever. A vascular disease. Arch. Intern. Med. 125:879-882.
11. Henderson, I. R., F. Navarro-Garcia, M. Desvaux, R. C. Fernandez, and D. Ala'Aldeen. 2004. Type V protein secretion pathway: the autotransporter story. Microbiol. Mol. Biol. Rev. 68:692-744.
12. Li, H., and D. H. Walker. 1998. rOmpA is a critical protein for the adhesion of Rickettsia rickettsii to host cells. Microb. Pathog. 24:289-298.
13. Martinez, J. J., and P. Cossart. 2004. Early signaling events involved in the entry of Rickettsia conorii into mammalian cells. J. Cell Sci. 117:5097-5106.
14. Martinez, J. J., M. A. Mulvey, J. D. Schilling, J. S. Pinkner, and S. J. Hultgren. 2000. Type 1 pilus-mediated bacterial invasion of bladder epithelial cells. EMBO J. 19:2803-2812.
15. Martinez, J. J., S. Seveau, E. Veiga, S. Matsuyama, and P. Cossart. 2005. Ku70, a component of DNA-dependent protein kinase, is a mammalian receptor for Rickettsia conorii. Cell 123:1013-1023.
16. Ngwamidiba, M., G. Blanc, D. Raoult, and P. E. Fournier. 2006. Sca1, a previously undescribed paralog from autotransporter protein-encoding genes in Rickettsia species. BMC Microbiol. 6:12.
17. Reed, L. J., and H. Muench. 1938. A simple method of estimating fifty percent endpoints. Am. J. Hyg. 27:493-497.
18. Uchiyama, T., H. Kawano, and Y. Kusuhara. 2006. The major outer membrane protein rOmpB of spotted fever group rickettsiae functions in the rickettsial adherence to and invasion of Vero cells. Microbes Infect. 8:801-809.
19. Vishwanath, S. 1991. Antigenic relationships among the rickettsiae of the spotted fever and typhus groups. FEMS Microbiol. Lett. 65:341-344.
20. Walker, D. H., and J. H. Gear. 1985. Correlation of the distribution of Rickettsia conorii, microscopic lesions, and clinical features in South African tick bite fever. Am. J. Trop. Med. Hyg. 34:361-371.
21. Walker, D. H., C. Occhino, G. R. Tringali, S. Di Rosa, and S. Mansueto. 1988. Pathogenesis of rickettsial eschars: the tache noire of boutonneuse fever. Hum. Pathol. 19:1449-1454.
22. Yagupsky, P., and B. Wolach. 1993. Fatal Israeli spotted fever in children. Clin. Infect. Dis. 17:850-853.