Regulation of ginsenoside and phytosterol biosynthesis by RNA interferences of squalene epoxidase gene in Panax ginseng

Phytochemistry

Volumn 71, Issue 1, 2010, Pages 36-46

  Han, Jung Yeon ^a   In, Jun Gyo ^b   Kwon, Yong Soo ^a   Choi, Yong Eui ^a  

^a Kangwon National University   (South Korea)

^b BioPia Research Institute   (South Korea)

Author keywords

Araliaceae; Ginseng; Panax ginseng; Phytosterol; RNA interference; Squalene epoxidase; Triterpene

Indexed keywords

ACETIC ACID DERIVATIVE; CYCLOPENTANE DERIVATIVE; GINSENOSIDE; JASMONIC ACID METHYL ESTER; MESSENGER RNA; OXYLIPIN; PHYTOSTEROL; RNA; SQUALENE MONOOXYGENASE;

ARTICLE; BIOSYNTHESIS; DRUG EFFECT; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENETICS; GINSENG; METABOLISM; PLANT GENE; PLANT MORPHOLOGY; RNA INTERFERENCE; SEQUENCE HOMOLOGY; UPREGULATION;

ACETIC ACIDS; CYCLOPENTANES; GENE EXPRESSION; GENE EXPRESSION REGULATION, PLANT; GENES, PLANT; GINSENOSIDES; OXYLIPINS; PANAX; PHYTOSTEROLS; PLANT STRUCTURES; RNA; RNA INTERFERENCE; RNA, MESSENGER; SEQUENCE HOMOLOGY, AMINO ACID; SQUALENE MONOOXYGENASE; UP-REGULATION;

ARALIACEAE; PANAX GINSENG;

EID: 72149121133     PISSN: 00319422     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.phytochem.2009.09.031     Document Type: Article

References (38)

1. Abe I., Rohmer M., and Prestwich G.D. Enzymatic cyclization of squalene and oxidosqualene to sterols and triterpenes. Chem. Rev. 93 (1993) 2189-2206
2. Benveniste P. Biosynthesis and accumulation of sterols. Annu. Rev. Plant Biol. 255 (2004) 429-457
3. Briskin D.P. Medicinal plants and phytomedicines. Linking plant biochemistry and physiology to human health. Plant Physiol. 124 (2000) 507-514
4. Choi Y.E., Yang D.C., Kusano T., and Sano H. Rapid and efficient Agrobacterium-mediated genetic transformation by plasmolyzing pretreatment of cotyledons in Panax ginseng. Plant Cell Rep. 20 (2001) 616-621
5. Chugh A., Ray A., and Gupta J.B. Squalene epoxidase as hypocholesterolemic drug target revisited. Prog. Lipid Res. 42 (2003) 37-50
6. Coleman C.I., Hebert J.H., and Reddy P. The effects of Panax ginseng on quality of life. J. Clin. Pharm. Ther. 28 (2003) 5-15
7. Eisel, D., Grunewald-Janho, S., Kruchen, B., 2000. Transcriptional labeling of RNA probes: procedures for non radioactive labeling and detection. In: DIG application manual. Roche Diagnostics Corporation, Germany, p. 65.
8. Favre B., and Ryder N.S. Characterization of squalene epoxidase activity from the dermatophyte Trichophyton rubrum and its inhibition by terbinafine and other antimycotic agents. Antimicrob. Agents 40 (1996) 443-447
9. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 (1985) 783-791
10. Gundlach H., Müller M.J., Kutchan T.M., and Zenk M.H. Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. Proc. Natl. Acad. Sci. USA 89 (1992) 2389-2393
11. Han J.Y., Jung S.J., Kim S.W., Kwon Y.S., Yi M.J., Yi J.S., and Choi Y.E. Induction of adventitious roots, analysis of ginsenoside and genes involved in triterpene biosynthesis in Panax ginseng. J. Plant Biol. 49 (2006) 26-33
12. Haralampidis K., Trojanowska M., and Osbourn A.E. Biosynthesis of triterpenoid saponin in plants. Adv. Biochem. Eng. Biotechnol. 75 (2002) 31-49
13. He F., Zhu Y., He M., and Zhang Y. Molecular cloning and characterization of the gene encoding squalene epoxidase in Panax notoginseng. Mitochond. DNA 19 (2008) 270-273
14. Horie M., Tsuchiya Y., Hayashi M., Iida Y., Iwasawa Y., Nagata Y., Sawasaki Y., Fukuzumi H., Kitani K., and Kamei T. NB-598: a potent competitive inhibitor of squalene epoxidase. J. Biol. Chem. 265 (1990) 18075-18078
15. Hostettmann K.A., and Marston A. Saponins: Chemistry and Pharmacology of Natural Products (1995), Cambridge University Press, Cambridge, United Kingdom
16. Jones P.J.H., MacDougall D.E., Ntanios F., and Vanstone C.A. Dietary phytosterols as cholesterol-lowering agents in humans. Can. J. Physiol. Pharmacol. 75 (1997) 217-227
17. Kohno Y., Egawa Y., Itoh S., Nagaoka S., Takahashi M., and Mukai K. Kinetic study of quenching reaction of singlet oxygen and scavenging reaction of free radical by squalene in n-butanol. Biochim. Biophys. Acta 1256 (1995) 52-56
18. Krogh A., Larsson B., von Heijne G., and Sonnhammer E.L. Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J. Mol. Biol. 305 (2001) 567-580
19. Landl K., Klösch B., and Turnowsky F. ERG1, encoding squalene epoxidase, is located on the right arm of chromosome VII of Saccharomyces cerevisiae. Yeast 12 (1996) 609-613
20. Lee M.H., Jeong J.H., Seo J.W., Shin C.G., Kim Y.S., In J.G., Yang D.C., Yi J.S., and Choi Y.E. Enhanced triterpene and phytosterol biosynthesis in Panax ginseng overexpressing squalene synthase gene. Plant Cell Physiol. 45 (2004) 976-984
21. Madey E., Nowack L.M., and Thompson J.E. Isolation and characterization of lipid in phloem sap of canola. Planta 214 (2002) 625-634
22. Murashige T., and Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue. Physiol. Plant 15 (1962) 473-497
23. Ogita S., Uefuji H., Yamaguchi Y., Koizumi N., and Sano H. RNA interference. Producing decaffeinated coffee plants. Nature 423 (2003) 823
24. Page R.D. TreeView: an application to display phylogenetic trees on personal computers. Comput. Appl. Biosci. 12 (1996) 357-358
25. Park J.D., Rhee D.K., and Lee Y.H. Biological activities and chemistry of saponins from Panax ginseng C.A. Meyer. Phytochem. Rev. 4 (2005) 159-175
26. Petranyi G., Ryder N.S., and Stutz A. Allylamine derivatives: new class of synthetic antifungal agents inhibiting fungal squalene epoxidase. Science 224 (1984) 1239-1241
27. Rasbery J.M., Shan H., LeClair R.J., Norman M., Matsuda S.P., and Bartel B. Arabidopsis thaliana squalene epoxidase 1 is essential for root and seed development. J. Biol. Chem. 282 (2007) 17002-17013
28. Ryder N.S. Squalene epoxidase as a target for the allylamines. Biochem. Soc. Trans. 19 (1991) 774-777
29. Sakakibara J., Watanabe R., Kanai Y., and Ono T. Molecular cloning and expression of rat squalene epoxidase. J. Biol. Chem. 270 (1995) 17-20
30. Samukawa K., Yamashita H., Matsuda H., and Kubo M. Simultaneous analysis of saponins in ginseng radix by high performance liquid chromatography. Chem. Pharm. Bull. 43 (1995) 137-141
31. Seo J.W., Jeong J.H., Shin C.G., Lo S.C., Han S.S., Yu K.W., Harada E., Han J.Y., and Choi Y.E. Overexpression of squalene synthase in Eleutherococcus senticosus increases phytosterol and triterpene accumulation. Phytochemistry 66 (2005) 869-877
32. Schaller H. The role of sterols in plant growth and development. Prog. Lipid Res. 42 (2003) 163-175
33. Shibata S. Chemistry and cancer preventing activity of ginseng saponins and some related terpenoid compounds. J. Kor. Med. Sci. 16 (2001) S28-37
34. Suzuki H., Achnine L., Xu R., Matsuda S.P.T., and Dixon R.A. A genomics approach to the early stages of triterpene saponin biosynthesis in Medicago truncatula. Plant J. 32 (2002) 1033-1048
35. Thompson J.D., Higgins D.G., and Gibson T.J. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl. Acids Res. 22 (1994) 4673-4680
36. Uchida H., Sugiyama R., Nakayachi O., Takemura M., and Ohyama K. Expression of the gene for sterol-biosynthesis enzyme squalene epoxidase in parenchyma cells of the oil plant, Euphorbia tirucalli. Planta 226 (2007) 1109-1115
37. Vogler B.K., Pittler M.H., and Ernst E. The efficacy of ginseng. A systematic review of randomised clinical trials. Eur. J. Clin. Pharmacol. 55 (1999) 567-575
38. Wen J., and Zimmer E.A. Phylogeny and biogeography of Panax L. (the ginseng genus, Araliaceae): inferences from ITS sequences of nuclear ribosomal DNA. Mol. Phylogenet. Evol. 6 (1996) 167-177