Rapid and efficient Agrobacterium-mediated transformation of Panax ginseng by plasmolyzing pre-treatment of cotyledons

Plant Cell Reports

Volumn 20, Issue 7, 2001, Pages 616-621

  Choi, Y E ^a   Yang, D C ^a   Kusano, T ^a   Sano, H ^a  

^a Chung Ang University   (South Korea)

Author keywords

Genetic transformation; Panax ginseng; Plasmolysis; Somatic embryogenesis

Indexed keywords

BETA GLUCURONIDASE; COCULTURE; COLOR; CONCENTRATION (PARAMETERS); DEVELOPMENTAL STAGE; GENETIC TRANSFORMATION; PLANT ANATOMY AND MORPHOLOGY; PLANT SEED; PLASMOLYSIS; POLYMERASE CHAIN REACTION; SOMATIC EMBRYOGENESIS; STAINING; SUCROSE; TRANSGENIC PLANT; TRANSIENT TRANSFECTION;

BACTERIA; CELLS; GENES; SUGAR (SUCROSE);

POLYMERASE CHAIN REACTION;

PLANTS (BOTANY);

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; BACTERIA (MICROORGANISMS); EMBRYOPHYTA; EUKARYOTA; PANAX GINSENG; RADIOBACTER; RHIZOBIUM; SPERMATOPHYTA;

EID: 0034772873     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002990100377     Document Type: Article

References (19)

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3. In vitro flowering of embryoids derived from mature root callus of ginseng (Panax ginseng)
4. Enhanced somatic single embryo formation by plasmolyzing pretreatment from cultured ginseng cotyledons
5. Regenerative ability of somatic single and multiple embryos from cotyledons of Korean ginseng on hormone-free medium
6. High efficiency plant production via direct somatic single embryogenesis from pre-plasmolysed cotyledons of Panax ginseng and possible dormancy of somatic embryos
7. Electroporation and PEG delivery of DNA into maize microspores
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9. Growth pattern and ginsenoside production of Agrobacterium-transformed Panax ginseng roots
10. A rapid and easy-handling procedure for isolation of DNA from rice, Arabidopsis and tobacco
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12. A revised medium for rapid growth and bioassays with tobacco tissue
13. Construction and expression in tobacco of a β-glucuronidase (GUS) reporter gene containing an intron within the coding sequence
14. Somatic embryogenesis and clonal multiplication of Panax ginseng
15. Plasmolysis of precultured immature embryos improves Agrobacterium mediated gene transfer to rice (Oryza sativa L.)
16. Transient expression of β-glucuronidase following biolistic delivery of foreign DNA into coffee tissues
17. Plasmolysis facilitates the accumulation of protein and DNA into extra-plasmalemma spaces of intact plant cells
18. Production of transgenic plants of Panax ginseng from Agrobacterium-transformed hairy roots
19. Saponin production by cultures of Panax ginseng transformed with Agrobacterium rhizogenes