Analysis of activity and inhibition of oxygen-dependent enzymes by optical respirometry on the LightCycler system

Analytical Biochemistry

Volumn 397, Issue 2, 2010, Pages 144-151

  Zitova, Alice ^a   Hynes, James ^b   Kollar, Julien ^a   Borisov, Sergey M ^c   Klimant, Ingo ^c   Papkovsky, Dmitri B ^a  

^a UNIVERSITY COLLEGE CORK   (Ireland)

^b Luxcel Biosciences Ltd   (Ireland)

^c GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

Cyclooxygenase; Cytochrome P450; Enzymatic assays; LightCycler; Monoamine oxidase; Optical oxygen respirometry; Oxygen sensitive probes; Phosphorescence

Indexed keywords

DETOXIFICATION; DISEASES; DRUG DELIVERY; ENZYME INHIBITION; OXYGEN; PHOSPHORESCENCE; PROBES;

CYCLOOXYGENASES; CYTOCHROME P450; ENZYMATIC ASSAY; LIGHTCYCLER; MONOAMINE OXIDASE; RESPIROMETRY; SENSITIVE PROBE;

IRIDIUM COMPOUNDS;

5 (4 CHLOROPHENYL) 1 (4 METHOXYPHENYL) 3 TRIFLUOROMETHYL 1H PYRAZOLE; AMINE OXIDASE (FLAVIN CONTAINING); CLORGYLINE; CYTOCHROME P450; IRIDIUM; KETOPROFEN; NANOPARTICLE; NIFLUMIC ACID; NIMESULIDE; OXYGEN; PHENYLBUTAZONE; PORPHYRIN; PROSTAGLANDIN SYNTHASE; SELEGILINE; TOLOXATONE;

ANALYTIC METHOD; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME INHIBITION; ENZYME MECHANISM; MACROMOLECULE; MATERIAL COATING; MOLECULAR PROBE; OPTICS; OXYGEN CONSUMPTION; PHOSPHORESCENCE; PRIORITY JOURNAL; RESPIROMETER; RESPIROMETRY; SOLID STATE;

EID: 72049119622     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.10.029     Document Type: Article

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