Cloning, overexpression, purification, and site-directed mutagenesis of xylitol-2-dehydrogenase from Candida albicans

Journal of Molecular Catalysis B: Enzymatic

Volumn 62, Issue 1, 2010, Pages 40-45

  Hong, Shiqi ^a   Wu, Jinchuan ^a   Zhao, Hua ^a  

^a INSTITUTE OF CHEMICAL AND ENGINEERING SCIENCES   (Singapore)

Author keywords

Cofactor imbalance; Site directed mutagenesis; Xylitol 2 dehydrogenase

Indexed keywords

CANDIDA ALBICANS; CATALYTIC EFFICIENCIES; COFACTORS; MEDIUM-CHAIN ALCOHOLS; OVER-EXPRESSION; SITE DIRECTED MUTAGENESIS;

CLONING; ENZYMES; ESCHERICHIA COLI; MUTAGENESIS; PURIFICATION;

SUGAR SUBSTITUTES;

ALCOHOL DEHYDROGENASE; ARGININE; ASPARTIC ACID; CYSTEINE; ISOLEUCINE; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; OXIDOREDUCTASE; PHENYLALANINE; RECOMBINANT PROTEIN; SERINE; TYROSINE; UNCLASSIFIED DRUG; XYLITOL 2 DEHYDROGENASE;

ARTICLE; CANDIDA ALBICANS; CONTROLLED STUDY; ESCHERICHIA COLI; GENE MUTATION; GENE OVEREXPRESSION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN PURIFICATION; SITE DIRECTED MUTAGENESIS;

CANDIDA ALBICANS; ESCHERICHIA COLI;

EID: 71849107681     PISSN: 13811177     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molcatb.2009.08.013     Document Type: Article

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